Multiplex Immunodetection

Multiplex Immunodetection

Maximize Targets, Minimize Cross-Reactivity

We offer in-house–produced and thoroughly tested antibodies conjugated to a variety of fluorophores, as well as extracellular-epitope reagents optimized for multiplex immunofluorescence, immunocolocalization, and high-parameter imaging. Guinea pig–derived antibodies are also available, providing additional species flexibility to help researchers build highly specific panels with minimal cross-reactivity. All antibodies are rigorously validated for applications including immunohistochemistry (IHC), immunocytochemistry (ICC), western blot (WB), and flow cytometry.

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FAQs

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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

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322 Results
  • Guinea pig Anti-Connexin-43 Antibody
    Guinea pig Anti-Connexin-43 Antibody
    A Guinea Pig Polyclonal Antibody to Connexin-43
    • Product Code: ACC-201-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to Connexin-43
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: CX43, Gap junction α1 protein, GJA1, GJAL, Gap junction 43 kDa heart protein
  • Guinea pig Anti-D<sub>1</sub> Dopamine Receptor Antibody
    Guinea pig Anti-D1 Dopamine Receptor Antibody
    A Guinea Pig Polyclonal Antibody to D1 Dopamine Receptor
    • Product Code: ADR-001-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to D1 Dopamine Receptor
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: DRD1, D(1A) dopamine receptor
  • Guinea Pig Anti-Dopamine Transporter (DAT) (extracellular) Antibody
    Guinea Pig Anti-Dopamine Transporter (DAT) (extracellular) Antibody
    A Guinea Pig Polyclonal Antibody to Dopamine Transporter (DAT)
    • Product Code: AMT-003-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to Dopamine Transporter (DAT)
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: Sodium-dependent dopamine transporter, DA transporter, Solute carrier family 6 member 3, SLC6A3
  • Guinea Pig Anti-EAAT2 (GLT-1) (extracellular) Antibody
    Guinea Pig Anti-EAAT2 (GLT-1) (extracellular) Antibody
    A Guinea Pig Polyclonal Antibody to Excitatory Amino Acid Transporter 2 (EAAT2, GLT-1)
    • Product Code: AGC-022-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to Excitatory Amino Acid Transporter 2 (EAAT2, GLT-1)
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: Excitatory amino acid transporter 2, Sodium-dependent glutamate/aspartate transporter 2, Glutamate/Aspartate Transporter II, Solute Carrier Family 1 Member 2, SLC1A2
  • Guinea pig Anti-EMR1 (ADGRE1) (extracellular) Antibody
    Guinea pig Anti-EMR1 (ADGRE1) (extracellular) Antibody
    A Guinea Pig Polyclonal Antibody to EMR1
    • Product Code: AER-051-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to EMR1
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot
    • Target: MR1 hormone receptor, EGF-like module-containing mucin-like hormone receptor-like 1, Adhesion G protein-coupled receptor E1, TM7LN3, F4/80, GPF480
  • Guinea pig Anti-Endothelin Receptor B Antibody
    Guinea pig Anti-Endothelin Receptor B Antibody
    A Guinea Pig Polyclonal Antibody to Endothelin Receptor B
    • Product Code: AER-002-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to Endothelin Receptor B
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot
    • Target: EDNRB, ET-B, ETB, ETRB, Endothelin B receptor, Endothelin receptor non-selective type
  • Guinea Pig Anti-GABA Transporter 1 (GAT-1) (extracellular) Antibody
    Guinea Pig Anti-GABA Transporter 1 (GAT-1) (extracellular) Antibody
    A Guinea Pig Polyclonal Antibody to GABA Transporter 1 (GAT-1) (extracellular)
    • Product Code: AGT-001-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to GABA Transporter 1 (GAT-1) (extracellular)
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: GABATR, GABT1, GABATHG, Na+/Cl-- dependent GABA transporter 1, Solute Carrier Family 6 Member 1, SLC6A1
  • Guinea pig Anti-GABA(A) α1 Receptor (extracellular) Antibody
    Guinea pig Anti-GABA(A) α1 Receptor (extracellular) Antibody
    A Guinea Pig Polyclonal Antibody to GABA(A) α1 Receptor
    • Product Code: AGA-001-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to GABA(A) α1 Receptor
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: γ-Aminobutyric acid receptor type A subunit α1, GABRA1
  • Guinea Pig Anti-GAP43 Antibody
    Guinea Pig Anti-GAP43 Antibody
    A Guinea Pig Polyclonal Antibody to GAP43
    • Product Code: ANR-016-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to GAP43
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: Axonal membrane protein GAP-43, Growth-associated protein 43, Neuromodulin, Neural phosphoprotein B-50
  • Guinea Pig Anti-Gephyrin Antibody
    Guinea Pig Anti-Gephyrin Antibody
    A Guinea Pig Polyclonal Antibody to Gephyrin
    • Product Code: AIP-005-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to Gephyrin
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: GPHN, Putative glycine receptor-tubulin linker protein
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