Multiplex Immunodetection

Multiplex Immunodetection

Maximize Targets, Minimize Cross-Reactivity

We offer in-house–produced and thoroughly tested antibodies conjugated to a variety of fluorophores, as well as extracellular-epitope reagents optimized for multiplex immunofluorescence, immunocolocalization, and high-parameter imaging. Guinea pig–derived antibodies are also available, providing additional species flexibility to help researchers build highly specific panels with minimal cross-reactivity. All antibodies are rigorously validated for applications including immunohistochemistry (IHC), immunocytochemistry (ICC), western blot (WB), and flow cytometry.

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FAQs

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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

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322 Results
  • Anti-CALHM1-ATTO Fluor-594 Antibody
    Anti-CALHM1-ATTO Fluor-594 Antibody
    A Rabbit Polyclonal Antibody to Calcium Homeostasis Modulator 1 Conjugated to the Fluorescent Dye ATTO-594
    • Product Code: ACC-101-AR
    • Short Description: A Rabbit Polyclonal Antibody to Calcium Homeostasis Modulator 1 Conjugated to the Fluorescent Dye ATTO-594
    • Host: Rabbit
    • Label: ATTO 594
    • Reactivity: Human, Rat, Mouse
    • Applications: Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Calcium homeostasis modulator 1, FAM26C
  • Anti-CALHM2 (extracellular)-FITC Antibody
    Anti-CALHM2 (extracellular)-FITC Antibody
    A Rabbit Polyclonal antibody to CALHM2 (extracellular) conjugated to the fluorescent dye FITC
    • Product Code: ACC-142-F
    • Short Description: A Rabbit Polyclonal antibody to CALHM2 (extracellular) conjugated to the fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Calcium homeostasis modulator protein 2, Protein FAM26B, FAM26B
  • Anti-Calnexin-ATTO Fluor-594 Antibody
    Anti-Calnexin-ATTO Fluor-594 Antibody
    A Rabbit Polyclonal Antibody to Calnexin Conjugated to the Fluorescent Dye ATTO-594
    • Product Code: ACS-009-AR
    • Short Description: A Rabbit Polyclonal Antibody to Calnexin Conjugated to the Fluorescent Dye ATTO-594
    • Host: Rabbit
    • Label: ATTO 594
    • Reactivity: Human, Rat, Mouse
    • Applications: Immunofluorescence, Immunohistochemistry
    • Target: Canx, Calx, CNX, Major histocompatibility complex class I antigen-binding protein p88, p90, IP90
  • Anti-Cannabinoid Receptor 1 (extracellular)-FITC Antibody
    Anti-Cannabinoid Receptor 1 (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to Cannabinoid Receptor 1 Conjugated to the Fluorescent Dye FITC
    • Product Code: ACR-001-F
    • Short Description: A Rabbit Polyclonal Antibody to Cannabinoid Receptor 1 Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: CB1, CB-R, CNR1, CB1R, CANN6, Brain-type cannabinoid receptor, Central cannabinoid receptor
  • Anti-Ca<sub>V</sub>1.2 (CACNA1C)-ATTO Fluor-488 Antibody
    KO Validate'
    Anti-CaV1.2 (CACNA1C)-ATTO Fluor-488 Antibody
    A Rabbit Polyclonal Antibody to CaV1.2 (CACNA1C) Channel Conjugated to the Fluorescent Dye ATTO-488
    • Product Code: ACC-003-AG
    • Short Description: A Rabbit Polyclonal Antibody to CaV1.2 (CACNA1C) Channel Conjugated to the Fluorescent Dye ATTO-488
    • Host: Rabbit
    • Label: ATTO 488
    • Reactivity: Human, Rat, Mouse
    • Applications: Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Voltage-dependent L-type calcium channel subunit α1C
  • Anti-Ca<sub>V</sub>1.3 (CACNA1D) (extracellular)-FITC Antibody
    Anti-CaV1.3 (CACNA1D) (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to CaV1.3 (CACNA1D) (extracellular) conjugated to the fluorescent dye FITC
    • Product Code: ACC-311-F
    • Short Description: A Rabbit Polyclonal Antibody to CaV1.3 (CACNA1D) (extracellular) conjugated to the fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Voltage-dependent L-type calcium channel subunit α1D
  • Anti-CD39 (extracellular)-FITC Antibody
    Anti-CD39 (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to CD39 Conjugated to the Fluorescent Dye FITC
    • Product Code: ANT-065-F
    • Short Description: A Rabbit Polyclonal Antibody to CD39 Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Ectonucleoside Triphosphate Diphosphohydrolase 1, Ecto-ATP diphosphohydrolase 1, Lymphoid cell activation antigen, ENTPD1
  • Anti-CD56/NCAM1 (extracellular)-ATTO Fluor-488 Antibody
    Anti-CD56/NCAM1 (extracellular)-ATTO Fluor-488 Antibody
    A Rabbit Polyclonal Antibody to CD56/NCAM1
    • Product Code: ANR-041-AG
    • Short Description: A Rabbit Polyclonal Antibody to CD56/NCAM1
    • Host: Rabbit
    • Label: ATTO 488
    • Reactivity: Human, Rat, Mouse
    • Applications: Immunohistochemistry
    • Target: NCAM-1, CD56
  • Anti-CD63 (extracellular)-FITC Antibody
    Anti-CD63 (extracellular)-FITC Antibody
    A Rabbit Polyclonal antibody to CD63 (extracellular) conjugated to the fluorescent dye FITC
    • Product Code: ANR-210-F
    • Short Description: A Rabbit Polyclonal antibody to CD63 (extracellular) conjugated to the fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Tetraspanin-30, TSPAN30, Lysosomal-Associated Membrane Protein 3, LAMP-3, Granulophysin, Melanoma-Associated Antigen ME491
  • Anti-CD73 (extracellular)-FITC Antibody
    Anti-CD73 (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to CD73 (extracellular) conjugated to the fluorescent dye FITC
    • Product Code: ANT-066-F
    • Short Description: A Rabbit Polyclonal Antibody to CD73 (extracellular) conjugated to the fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Flow cytometry
    • Target: Ecto-5-nucleotidase, E5NT, 5'-Nucleotidase, 5'-NT, NT5E, ENT
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