Multiplex Immunodetection

Multiplex Immunodetection

Maximize Targets, Minimize Cross-Reactivity

We offer in-house–produced and thoroughly tested antibodies conjugated to a variety of fluorophores, as well as extracellular-epitope reagents optimized for multiplex immunofluorescence, immunocolocalization, and high-parameter imaging. Guinea pig–derived antibodies are also available, providing additional species flexibility to help researchers build highly specific panels with minimal cross-reactivity. All antibodies are rigorously validated for applications including immunohistochemistry (IHC), immunocytochemistry (ICC), western blot (WB), and flow cytometry.

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FAQs

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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

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322 Results
  • Anti-GPR56 (extracellular)-FITC Antibody
    Anti-GPR56 (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to GPR56 Conjugated to the Fluorescent Dye FITC
    • Product Code: AGR-047-F
    • Short Description: A Rabbit Polyclonal Antibody to GPR56 Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Flow cytometry
    • Target: G-protein coupled receptor 56, Adhesion G-protein coupled receptor G1, ADGRG1
  • Anti-GPR65 (TDAG8) (extracellular)-FITC Antibody
    Anti-GPR65 (TDAG8) (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to GPR65 (TDAG8) Conjugated to the Fluorescent Dye FITC
    • Product Code: AGR-043-F
    • Short Description: A Rabbit Polyclonal Antibody to GPR65 (TDAG8) Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: G-protein coupled receptor 65, T-cell death-associated gene 8 protein, Psychosine receptor
  • Anti-GPR68 (OGR1) (extracellular)-FITC Antibody
    Anti-GPR68 (OGR1) (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to GPR68 Conjugated to the Fluorescent Dye FITC
    • Product Code: AGR-042-F
    • Short Description: A Rabbit Polyclonal Antibody to GPR68 Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Acid-sensing G-protein coupled receptor 68, Ovarian cancer G-protein coupled receptor 1, Sphingosylphosphorylcholine receptor
  • Anti-GPR84 (extracellular)-FITC Antibody
    Anti-GPR84 (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to GPR84 Conjugated to the Fluorescent Dye FITC
    • Product Code: AGR-052-F
    • Short Description: A Rabbit Polyclonal Antibody to GPR84 Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: G-protein coupled receptor 84, Inflammation-related G-protein coupled receptor EX33
  • Anti-GPR91 (SUCNR1) (extracellular)-FITC Antibody
    Anti-GPR91 (SUCNR1) (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to GPR91 Conjugated to the Fluorescent Dye FITC
    • Product Code: ASR-090-F
    • Short Description: A Rabbit Polyclonal Antibody to GPR91 Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: G-protein coupled receptor 91, Succinate receptor 1
  • Anti-HCN2-ATTO Fluor-594 Antibody
    KO Validate'
    Anti-HCN2-ATTO Fluor-594 Antibody
    A Rabbit Polyclonal Antibody to HCN2 Conjugated to the Fluorescent Dye ATTO-594
    • Product Code: APC-030-AR
    • Short Description: A Rabbit Polyclonal Antibody to HCN2 Conjugated to the Fluorescent Dye ATTO-594
    • Host: Rabbit
    • Label: ATTO 594
    • Reactivity: Human, Rat, Mouse
    • Applications: Immunofluorescence, Immunohistochemistry
    • Target: Hyperpolarization-activated cyclic nucleotide-gated potassium channel 2, BCNG2, HAC-1
  • Anti-Histamine H1 Receptor/HRH1 (extracellular)-FITC Antibody
    Anti-Histamine H1 Receptor/HRH1 (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to H1R (extracellular) conjugated to the fluorescent dye FITC
    • Product Code: AHR-006-F
    • Short Description: A Rabbit Polyclonal Antibody to H1R (extracellular) conjugated to the fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Flow cytometry
    • Target: Histamine Receptor H1, H1R
  • Anti-Human Adenosine A<sub>2A</sub> Receptor (extracellular)-FITC Antibody
    Anti-Human Adenosine A2A Receptor (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to Adenosine A2A Receptor Conjugated to the Fluorescent Dye FITC
    • Product Code: AAR-007-F
    • Short Description: A Rabbit Polyclonal Antibody to Adenosine A2A Receptor Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human
    • Applications: Flow cytometry
    • Target: Adenosine receptor A2A, A2AR, ADORA2A
  • Anti-Human ASCT2/SLC1A5 (extracellular)-FITC Antibody
    KO Validate'
    Anti-Human ASCT2/SLC1A5 (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to SLC1A5 (extracellular) conjugated to the fluorescent dye FITC
    • Product Code: ANT-083-F
    • Short Description: A Rabbit Polyclonal Antibody to SLC1A5 (extracellular) conjugated to the fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human
    • Applications: Live cell imaging, Flow cytometry
    • Target: Alanine/Serine/Cysteine Transporter 2, Neutral amino acid transporter B(0), ATB(0), Sodium-dependent Neutral Amino Acid Transporter Type 2
  • Anti-Human BLT1 (extracellular)-ATTO Fluor-488 Antibody
    Anti-Human BLT1 (extracellular)-ATTO Fluor-488 Antibody
    A Rabbit Polyclonal Antibody to BLT1 Conjugated to the Fluorescent Dye ATTO-488
    • Product Code: ALR-001-AG
    • Short Description: A Rabbit Polyclonal Antibody to BLT1 Conjugated to the Fluorescent Dye ATTO-488
    • Host: Rabbit
    • Label: ATTO 488
    • Reactivity: Human
    • Applications: Flow cytometry
    • Target: Leukotriene B4 receptor 1, LTB4R, LTB4R1, CMKRL1, GPR16, P2Y7, P2RY7
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