Multiplex Immunodetection

Multiplex Immunodetection

Maximize Targets, Minimize Cross-Reactivity

We offer in-house–produced and thoroughly tested antibodies conjugated to a variety of fluorophores, as well as extracellular-epitope reagents optimized for multiplex immunofluorescence, immunocolocalization, and high-parameter imaging. Guinea pig–derived antibodies are also available, providing additional species flexibility to help researchers build highly specific panels with minimal cross-reactivity. All antibodies are rigorously validated for applications including immunohistochemistry (IHC), immunocytochemistry (ICC), western blot (WB), and flow cytometry.

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FAQs

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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

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322 Results
  • Anti-Human CX3CR1 (extracellular)-FITC Antibody
    Anti-Human CX3CR1 (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to CX3CR1 Conjugated to the Fluorescent Dye FITC
    • Product Code: ACR-059-F
    • Short Description: A Rabbit Polyclonal Antibody to CX3CR1 Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human
    • Applications: Live cell imaging, Flow cytometry
    • Target: Fractalkine Receptor, CX3C chemokine receptor 1, C-X3-C CKR-1, C-X3-C motif chemokine receptor 1, GPR13, CMKBRL1, GPRV28, V28, G-protein coupled receptor 13, CCRL1, Beta chemokine receptor-like 1
  • Anti-Human CXCR2 (extracellular)-ATTO Fluor-488 Antibody
    Anti-Human CXCR2 (extracellular)-ATTO Fluor-488 Antibody
    A Rabbit Polyclonal Antibody to CXCR2 Chemokine Receptor Conjugated to the Fluorescent Dye ATTO-488
    • Product Code: ACR-012-AG
    • Short Description: A Rabbit Polyclonal Antibody to CXCR2 Chemokine Receptor Conjugated to the Fluorescent Dye ATTO-488
    • Host: Rabbit
    • Label: ATTO 488
    • Reactivity: Human
    • Applications: Live cell imaging, Flow cytometry
    • Target: C-X-C chemokine receptor type 2, Interleukin-8 receptor B, IL-8R B, IL8RB, IL-8 receptor type 2, CD182
  • Anti-Human FPR1 (extracellular)-ATTO Fluor-488 Antibody
    Anti-Human FPR1 (extracellular)-ATTO Fluor-488 Antibody
    A Rabbit Polyclonal Antibody to FPR1 Conjugated to the Fluorescent Dye ATTO-488
    • Product Code: AFR-001-AG
    • Short Description: A Rabbit Polyclonal Antibody to FPR1 Conjugated to the Fluorescent Dye ATTO-488
    • Host: Rabbit
    • Label: ATTO 488
    • Reactivity: Human
    • Applications: Live cell imaging, Flow cytometry
    • Target: N-formyl peptide receptor 1, fMLP receptor
  • Anti-Human GLUT3 (extracellular)-FITC Antibody
    Anti-Human GLUT3 (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to GLUT3 Conjugated to the Fluorescent Dye FITC
    • Product Code: AGT-043-F
    • Short Description: A Rabbit Polyclonal Antibody to GLUT3 Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human
    • Applications: Flow cytometry
    • Target: Glucose transporter 3, Facilitated glucose transporter member 3, SLC2A3
  • Anti-Human GPR132/G2A (extracellular)-FITC Antibody
    Anti-Human GPR132/G2A (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to Human GPR132 (extracellular) conjugated to the fluorescent dye FITC
    • Product Code: AGR-046-F
    • Short Description: A Rabbit Polyclonal Antibody to Human GPR132 (extracellular) conjugated to the fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human
    • Applications: Live cell imaging, Flow cytometry
    • Target: G protein-coupled receptor 132, G2 accumulation protein, G2A
  • Anti-Human Orai1 (extracellular)-FITC Antibody
    Anti-Human Orai1 (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to Human Orai1 Channel Conjugated to the Fluorescent Dye FITC
    • Product Code: ACC-060-F
    • Short Description: A Rabbit Polyclonal Antibody to Human Orai1 Channel Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human
    • Applications: Flow cytometry
    • Target: TMEM142A, CRACM1, Calcium release-activated calcium channel protein 1
  • Anti-Human PAR1 (F2R) (extracellular)-FITC Antibody
    Anti-Human PAR1 (F2R) (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to Protease-Activated Receptor-1 Conjugated to the Fluorescent dye FITC
    • Product Code: APR-031-F
    • Short Description: A Rabbit Polyclonal Antibody to Protease-Activated Receptor-1 Conjugated to the Fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human
    • Applications: Live cell imaging, Flow cytometry
    • Target: Protease-activated receptor-1, PAR-1, Thrombin receptor, Coagulation factor II receptor, CF2R
  • Anti-Human PAR2/F2RL1 (extracellular)-FITC Antibody
    Anti-Human PAR2/F2RL1 (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to PAR2 (extracellular) conjugated to the fluorescent dye FITC
    • Product Code: APR-035-F
    • Short Description: A Rabbit Polyclonal Antibody to PAR2 (extracellular) conjugated to the fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human
    • Applications: Live cell imaging, Flow cytometry
    • Target: Protease-activated receptor-2, PAR-2, Thrombin receptor-like 1, Coagulation factor II receptor-like 1, GPR11
  • Anti-Human TRPV1 (extracellular)-FITC Antibody
    Anti-Human TRPV1 (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody Human TRPV1 (extracellular) conjugated to the fluorescent dye FITC
    • Product Code: ACC-334-F
    • Short Description: A Rabbit Polyclonal Antibody Human TRPV1 (extracellular) conjugated to the fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human
    • Applications: Live cell imaging, Flow cytometry
    • Target: Transient receptor potential cation channel subfamily V member 1, Capsaicin receptor, Vanilloid receptor 1, VR1, OTRPC1
  • Anti-Human TRPV2 (VRL1) (extracellular)-FITC Antibody
    Anti-Human TRPV2 (VRL1) (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to Human TRPV2 (extracellular) conjugated to the fluorescent dye FITC
    • Product Code: ACC-139-F
    • Short Description: A Rabbit Polyclonal Antibody to Human TRPV2 (extracellular) conjugated to the fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human
    • Applications: Live cell imaging, Flow cytometry
    • Target: Vanilloid receptor-like protein 1, VRL-1, Osm-9-like TRP channel 2, OTRPC2
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