Multiplex Immunodetection

Multiplex Immunodetection

Maximize Targets, Minimize Cross-Reactivity

We offer in-house–produced and thoroughly tested antibodies conjugated to a variety of fluorophores, as well as extracellular-epitope reagents optimized for multiplex immunofluorescence, immunocolocalization, and high-parameter imaging. Guinea pig–derived antibodies are also available, providing additional species flexibility to help researchers build highly specific panels with minimal cross-reactivity. All antibodies are rigorously validated for applications including immunohistochemistry (IHC), immunocytochemistry (ICC), western blot (WB), and flow cytometry.

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FAQs

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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

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322 Results
  • Anti-S1PR1 (EDG1) (extracellular)-FITC Antibody
    Anti-S1PR1 (EDG1) (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to Sphingosine 1-Phosphate Receptor 1 Conjugated to the Fluorescent Dye FITC
    • Product Code: ASR-011-F
    • Short Description: A Rabbit Polyclonal Antibody to Sphingosine 1-Phosphate Receptor 1 Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Sphingosine 1-phosphate receptor 1, S1P1, Endothelial differentiation G-protein coupled receptor 1, CHEDG1, CD363
  • Anti-S1PR3 (EDG3) (extracellular)-ATTO Fluor-488 Antibody
    Anti-S1PR3 (EDG3) (extracellular)-ATTO Fluor-488 Antibody
    A Rabbit Polyclonal Antibody to Sphingosine 1-Phosphate Receptor 3 Conjugated to the Fluorescent Dye ATTO-488
    • Product Code: ASR-013-AG
    • Short Description: A Rabbit Polyclonal Antibody to Sphingosine 1-Phosphate Receptor 3 Conjugated to the Fluorescent Dye ATTO-488
    • Host: Rabbit
    • Label: ATTO 488
    • Reactivity: Human, Rat, Mouse
    • Applications: Flow cytometry
    • Target: Sphingosine 1-phosphate receptor 3, S1P3, Endothelial differentiation G-protein coupled receptor 3, Lysophospholipid receptor B3, LPB3
  • Anti-S1PR3 (EDG3) (extracellular)-FITC Antibody
    Anti-S1PR3 (EDG3) (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to Sphingosine 1-Phosphate Receptor 3 Conjugated to the Fluorescent Dye FITC
    • Product Code: ASR-013-F
    • Short Description: A Rabbit Polyclonal Antibody to Sphingosine 1-Phosphate Receptor 3 Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Sphingosine 1-phosphate receptor 3, S1P3, Endothelial differentiation G-protein coupled receptor 3, Lysophospholipid receptor B3, LPB3
  • Anti-SCN1A (Na<sub>V</sub>1.1)-ATTO Fluor-594 Antibody
    KO Validate'
    Anti-SCN1A (NaV1.1)-ATTO Fluor-594 Antibody
    A Rabbit Polyclonal Antibody to SCN1A (NaV1.1) Channel Conjugated to the Fluorescent Dye ATTO-594
    • Product Code: ASC-001-AR
    • Short Description: A Rabbit Polyclonal Antibody to SCN1A (NaV1.1) Channel Conjugated to the Fluorescent Dye ATTO-594
    • Host: Rabbit
    • Label: ATTO 594
    • Reactivity: Human, Rat, Mouse
    • Applications: Immunofluorescence, Immunohistochemistry
    • Target: Brain type I sodium channel, BI, Sodium channel protein type 1 subunit α
  • Anti-SCN2A (Na<sub>V</sub>1.2)-ATTO Fluor-594 Antibody
    KO Validate'
    Anti-SCN2A (NaV1.2)-ATTO Fluor-594 Antibody
    A Rabbit Polyclonal Antibody to SCN2A (NaV1.2) Channel Conjugated to the Fluorescent Dye ATTO-594
    • Product Code: ASC-002-AR
    • Short Description: A Rabbit Polyclonal Antibody to SCN2A (NaV1.2) Channel Conjugated to the Fluorescent Dye ATTO-594
    • Host: Rabbit
    • Label: ATTO 594
    • Reactivity: Human, Rat, Mouse
    • Applications: Immunofluorescence, Immunohistochemistry
    • Target: BII, Brain type II Na+ channel, Sodium channel protein type 2 subunit alpha
  • Anti-Semaphorin 4D (SEMA4D) (extracellular)-FITC Antibody
    Anti-Semaphorin 4D (SEMA4D) (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to Semaphorin 4D Conjugated to the Fluorescent Dye FITC
    • Product Code: ASR-064-F
    • Short Description: A Rabbit Polyclonal Antibody to Semaphorin 4D Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Flow cytometry
    • Target: CD100, Semaphorin-J
  • Anti-Serotonin Transporter (SERT) (extracellular)-FITC Antibody
    Anti-Serotonin Transporter (SERT) (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to Serotonin Transporter (SERT) Conjugated to the Fluorescent Dye FITC
    • Product Code: AMT-004-F
    • Short Description: A Rabbit Polyclonal Antibody to Serotonin Transporter (SERT) Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Sodium-dependent serotonin transporter, 5-HT transporter, 5-HTT, 5-HTTLPR, SLC6A4
  • Anti-SLC11A1/NRAMP1 (extracellular)-FITC Antibody
    Anti-SLC11A1/NRAMP1 (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to SLC11A1/NRAMP1   (extracellular) conjugated to the fluorescent dye FITC
    • Product Code: ANT-201-F
    • Short Description: A Rabbit Polyclonal Antibody to SLC11A1/NRAMP1   (extracellular) conjugated to the fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Natural Resistance-Associated Macrophage Protein 1, LSH, Solute Carrier Family 11 Member 1
  • Anti-SLC7A2 (extracellular)-FITC Antibody
    Anti-SLC7A2 (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to Cationic Amino Acid Transporter 2 (SLC7A2) Conjugated to the Fluorescent Dye FITC
    • Product Code: ANT-103-F
    • Short Description: A Rabbit Polyclonal Antibody to Cationic Amino Acid Transporter 2 (SLC7A2) Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Flow cytometry
    • Target: Cationic amino acid transporter 2, CAT-2, Low affinity cationic amino acid transporter 2, ATRC2, Solute carrier family 7 member 2
  • Anti-Sortilin (extracellular)-FITC Antibody
    Anti-Sortilin (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to Sortilin Conjugated to the Fluorescent Dye FITC
    • Product Code: ANT-009-F
    • Short Description: A Rabbit Polyclonal Antibody to Sortilin Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Flow cytometry
    • Target: Sortilin 1, SORT1, Neurotensin receptor 3, NTR3, NT3, Glycoprotein 95, Gp95
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