Multiplex Immunodetection

Multiplex Immunodetection

Maximize Targets, Minimize Cross-Reactivity

We offer in-house–produced and thoroughly tested antibodies conjugated to a variety of fluorophores, as well as extracellular-epitope reagents optimized for multiplex immunofluorescence, immunocolocalization, and high-parameter imaging. Guinea pig–derived antibodies are also available, providing additional species flexibility to help researchers build highly specific panels with minimal cross-reactivity. All antibodies are rigorously validated for applications including immunohistochemistry (IHC), immunocytochemistry (ICC), western blot (WB), and flow cytometry.

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FAQs

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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

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322 Results
  • Anti-TRPV4 (extracellular)-FITC Antibody
    Anti-TRPV4 (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to TRPV4 Conjugated to the Fluorescent Dye FITC
    • Product Code: ACC-124-F
    • Short Description: A Rabbit Polyclonal Antibody to TRPV4 Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: OTRPC4, VRL-2, VR-OAC, TRP12, Osm-9-Like TRP Channel 4
  • Anti-TRPV4-ATTO Fluor-550 Antibody
    KO Validate'
    Anti-TRPV4-ATTO Fluor-550 Antibody
    A Rabbit Polyclonal Antibody to TRPV4 Channel Conjugated to the Fluorescent Dye ATTO-550
    • Product Code: ACC-034-AO
    • Short Description: A Rabbit Polyclonal Antibody to TRPV4 Channel Conjugated to the Fluorescent Dye ATTO-550
    • Host: Rabbit
    • Label: ATTO 550
    • Reactivity: Human, Rat, Mouse
    • Applications: Immunofluorescence, Immunohistochemistry
    • Target: OTRPC4, VRL-2, VR-OAC, TRP12
  • Anti-TSH Receptor (TSHR) (extracellular)-ATTO Fluor-488 Antibody
    Anti-TSH Receptor (TSHR) (extracellular)-ATTO Fluor-488 Antibody
    A Rabbit Polyclonal Antibody to TSH Receptor Conjugated to the Fluorescent Dye ATTO-488
    • Product Code: ATR-006-AG
    • Short Description: A Rabbit Polyclonal Antibody to TSH Receptor Conjugated to the Fluorescent Dye ATTO-488
    • Host: Rabbit
    • Label: ATTO 488
    • Reactivity: Human, Rat, Mouse
    • Applications: Immunofluorescence, Immunohistochemistry
    • Target: Thyrotropin receptor, Thyroid-stimulating hormone receptor, TSH-R
  • Anti-Vesicular GABA Transporter (VGAT)-ATTO Fluor-594 Antibody
    Anti-Vesicular GABA Transporter (VGAT)-ATTO Fluor-594 Antibody
    A Rabbit Polyclonal Antibody to Vesicular GABA Transporter Conjugated to the Fluorescent Dye ATTO-594
    • Product Code: AGT-005-AR
    • Short Description: A Rabbit Polyclonal Antibody to Vesicular GABA Transporter Conjugated to the Fluorescent Dye ATTO-594
    • Host: Rabbit
    • Label: ATTO 594
    • Reactivity: Human, Rat, Mouse
    • Applications: Immunofluorescence, Immunohistochemistry
    • Target: Vesicular inhibitory amino acid transporter, VIAAT, GABA and glycine transporter, SLC32A1
  • Anti-VGLUT2-ATTO Fluor-594 Antibody
    Anti-VGLUT2-ATTO Fluor-594 Antibody
    A Rabbit Polyclonal Antibody to Vesicular Glutamate Transporter 2 Conjugated to the Fluorescent Dye ATTO-594
    • Product Code: AGC-036-AR
    • Short Description: A Rabbit Polyclonal Antibody to Vesicular Glutamate Transporter 2 Conjugated to the Fluorescent Dye ATTO-594
    • Host: Rabbit
    • Label: ATTO 594
    • Reactivity: Human, Rat, Mouse
    • Applications: Immunofluorescence, Immunohistochemistry
    • Target: Vesicular glutamate transporter 2, Differentiation-associated Na+-dependent inorganic phosphate cotransporter, DNPI, SLC17A6
  • Anti-VMAT2-ATTO Fluor-488 Antibody
    Anti-VMAT2-ATTO Fluor-488 Antibody
    A Rabbit Polyclonal Antibody to Vesicular Monoamine Transporter 2 Conjugated to the Fluorescent Dye ATTO-488
    • Product Code: AMT-006-AG
    • Short Description: A Rabbit Polyclonal Antibody to Vesicular Monoamine Transporter 2 Conjugated to the Fluorescent Dye ATTO-488
    • Host: Rabbit
    • Label: ATTO 488
    • Reactivity: Human, Rat, Mouse
    • Applications: Immunofluorescence, Immunohistochemistry
    • Target: Vesicular monoamine transporter 2, SLC18A2, SVAT, VAT2
  • Anti-VPAC1 (VIPR1) (extracellular)-FITC Antibody
    Anti-VPAC1 (VIPR1) (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to VPAC1 Receptor Conjugated to the Fluorescent Dye FITC
    • Product Code: AVR-001-F
    • Short Description: A Rabbit Polyclonal Antibody to VPAC1 Receptor Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: VIP and PACAP receptor 1, Vasoactive intestinal polypeptide receptor 1, Pituitary adenylate cyclase-activating polypeptide type II receptor, PACAP type II receptor, PACAP-R2
  • Anti-xCT/SLC7A11 (extracellular)-FITC Antibody
    Anti-xCT/SLC7A11 (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to Cystine/Glutamate Transporter (xCT) Conjugated to the Fluorescent dye FITC
    • Product Code: ANT-111-F
    • Short Description: A Rabbit Polyclonal Antibody to Cystine/Glutamate Transporter (xCT) Conjugated to the Fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Flow cytometry
    • Target: Cystine/glutamate transporter, Amino acid transport system xc-, Solute carrier family 7 member 11, Calcium channel blocker resistance protein CCBR1
  • Anti-ZIP6/SLC39A6 (extracellular)-FITC Antibody
    Anti-ZIP6/SLC39A6 (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to ZIP6 (extracellular) conjugated to the fluorescent dye FITC
    • Product Code: AZT-006-F
    • Short Description: A Rabbit Polyclonal Antibody to ZIP6 (extracellular) conjugated to the fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Zinc transporter ZIP6, Zrt- and Irt-like protein 6, Estrogen-regulated protein LIV-1, LIV1, Solute carrier family 39 member 6
  • Anti-ZIP8 (SLC39A8) (extracellular)-FITC Antibody
    Anti-ZIP8 (SLC39A8) (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to Zinc Transporter ZIP8 Conjugated to the Fluorescent Dye FITC
    • Product Code: AZT-008-F
    • Short Description: A Rabbit Polyclonal Antibody to Zinc Transporter ZIP8 Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Flow cytometry
    • Target: Zinc transporter ZIP8, Zrt- and Irt-like protein 8, Solute carrier family 39 member 8, BIGM103
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