Multiplex Immunodetection

Multiplex Immunodetection

Maximize Targets, Minimize Cross-Reactivity

We offer in-house–produced and thoroughly tested antibodies conjugated to a variety of fluorophores, as well as extracellular-epitope reagents optimized for multiplex immunofluorescence, immunocolocalization, and high-parameter imaging. Guinea pig–derived antibodies are also available, providing additional species flexibility to help researchers build highly specific panels with minimal cross-reactivity. All antibodies are rigorously validated for applications including immunohistochemistry (IHC), immunocytochemistry (ICC), western blot (WB), and flow cytometry.

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FAQs

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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

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322 Results
  • Guinea Pig Anti-GFAP Antibody
    Guinea Pig Anti-GFAP Antibody
    A Guinea Pig Polyclonal Antibody to Glial Fibrillary Acidic Protein
    • Product Code: AFP-001-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to Glial Fibrillary Acidic Protein
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: Glial fibrillary acidic protein
  • Guinea pig Anti-GIRK2 (K<sub>ir</sub>3.2) Antibody
    Guinea pig Anti-GIRK2 (Kir3.2) Antibody
    A Guinea Pig Polyclonal Antibody to GIRK2 (Kir3.2) Channel
    • Product Code: APC-006-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to GIRK2 (Kir3.2) Channel
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: G protein-activated inward rectifier potassium channel 2, Kcnj6
  • Guinea pig Anti-GluR1 (GluA1) (extracellular) Antibody
    Guinea pig Anti-GluR1 (GluA1) (extracellular) Antibody
    A Guinea Pig Polyclonal Antibody to AMPA-Selective Glutamate Receptor 1 (GluR1)
    • Product Code: AGC-004-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to AMPA-Selective Glutamate Receptor 1 (GluR1)
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: AMPA Receptor 1, Glutamate receptor 1, Ionotropic glutamate receptor 1, AMPA-selective glutamate receptor 1, GRIA1, GluR-A, GluR-K1
  • Guinea pig Anti-GluR2 (GluA2) (extracellular) Antibody
    Guinea pig Anti-GluR2 (GluA2) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to AMPA-Selective Glutamate Receptor 2 (GluR2)
    • Product Code: AGC-005-GP
    • Short Description: A Rabbit Polyclonal Antibody to AMPA-Selective Glutamate Receptor 2 (GluR2)
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence
    • Target: AMPA receptor 2, Glutamate receptor 2, Ionotropic glutamate receptor 2, AMPA-selective glutamate receptor 2, GRIA2, GluR-B, GluR-K2
  • Guinea pig Anti-GluR3 (GluA3) (extracellular) Antibody
    Guinea pig Anti-GluR3 (GluA3) (extracellular) Antibody
    A Guinea Pig Polyclonal Antibody to AMPA-Selective Glutamate Receptor 3 (GluR3)
    • Product Code: AGC-010-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to AMPA-Selective Glutamate Receptor 3 (GluR3)
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: AMPA receptor 3, Glutamate receptor 3, Ionotropic glutamate receptor 3, AMPA-selective glutamate receptor 3, GRIA3, GluR-C, GluR-K3
  • Guinea pig Anti-HCN1 Antibody
    Guinea pig Anti-HCN1 Antibody
    A Guinea Pig Polyclonal Antibody to HCN1 Channel
    • Product Code: APC-056-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to HCN1 Channel
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Hyperpolarization-activated cyclic nucleotide-gated potassium channel 1, HAC2, Brain cyclic nucleotide-gated channel 1, BCNG1, Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1
  • Guinea pig Anti-HCN4 Antibody
    Guinea pig Anti-HCN4 Antibody
    A Guinea Pig Polyclonal Antibody to HCN4 Channel
    • Product Code: APC-052-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to HCN4 Channel
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Hyperpolarization-activated cyclic nucleotide-gated potassium channel 4
  • Guinea Pig Anti-Homer1 Antibody
    Guinea Pig Anti-Homer1 Antibody
    A Guinea Pig Polyclonal Antibody to Homer1
    • Product Code: APZ-026-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to Homer1
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: Homer protein homolog 1, Homer scaffolding protein 1, Vesl-1, Ves 1, PSD-Zip45, SYN47
  • Guinea Pig Anti-IBA1/AIF1 Antibody
    Guinea Pig Anti-IBA1/AIF1 Antibody
    A Guinea Pig Polyclonal Antibody to IBA1/AIF1
    • Product Code: ACS-010-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to IBA1/AIF1
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: Ionized calcium-binding adapter molecule 1, Allograft Inflammatory Factor 1, MRF-1, Microglia response factor
  • Guinea pig Anti-KCNK2 (TREK-1) Antibody
    Guinea pig Anti-KCNK2 (TREK-1) Antibody
    A Guinea Pig Polyclonal Antibody to KCNK2 Channel
    • Product Code: APC-047-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to KCNK2 Channel
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Potassium channel subfamily K member 2, Outward rectifying potassium channel protein TREK-1, Twik-related K+ channel 1, K2P2.1, TPKC1
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