Multiplex Immunodetection

Multiplex Immunodetection

Maximize Targets, Minimize Cross-Reactivity

We offer in-house–produced and thoroughly tested antibodies conjugated to a variety of fluorophores, as well as extracellular-epitope reagents optimized for multiplex immunofluorescence, immunocolocalization, and high-parameter imaging. Guinea pig–derived antibodies are also available, providing additional species flexibility to help researchers build highly specific panels with minimal cross-reactivity. All antibodies are rigorously validated for applications including immunohistochemistry (IHC), immunocytochemistry (ICC), western blot (WB), and flow cytometry.

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FAQs

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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

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322 Results
  • Guinea pig Anti-KCNMA1 (K<sub>Ca</sub>1.1) (1097-1196) Antibody
    Guinea pig Anti-KCNMA1 (KCa1.1) (1097-1196) Antibody
    A Guinea Pig Polyclonal Antibody to KCNMA1 (KCa1.1) Channel
    • Product Code: APC-021-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to KCNMA1 (KCa1.1) Channel
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot
    • Target: Large conductance calcium-activated potassium channel subfamily M subunit alpha-1, BKCa alpha, Maxi K+, Slo1
  • Guinea pig Anti-KCNN2 (K<sub>Ca</sub>2.2, SK2) Antibody
    Guinea pig Anti-KCNN2 (KCa2.2, SK2) Antibody
    A Guinea Pig Polyclonal Antibody to KCNN2 (KCa2.2, SK2) Channel
    • Product Code: APC-028-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to KCNN2 (KCa2.2, SK2) Channel
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot
    • Target: SKCa2, Small conductance calcium-activated potassium channel protein 2, Apamin-sensitive small conductance Ca2+-dependent K+ channel
  • Guinea pig Anti-KCNQ1 Antibody
    Guinea pig Anti-KCNQ1 Antibody
    A Guinea Pig Polyclonal Antibody to KCNQ1 (KV7.1) Channel
    • Product Code: APC-022-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to KCNQ1 (KV7.1) Channel
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: KV7.1, Voltage-gated potassium channel QKT subfamily member 1, KVLQT1
  • Guinea pig Anti-KCNQ2 Antibody
    Guinea pig Anti-KCNQ2 Antibody
    A Guinea Pig Polyclonal Antibody to KCNQ2 (KV7.2) Channel
    • Product Code: APC-050-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to KCNQ2 (KV7.2) Channel
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: KV7.2, Voltage-gated potassium channel QKT subfamily member 2, KvLQT2
  • Guinea pig Anti-K<sub>ir</sub>2.1/KCNJ2 Antibody
    Guinea pig Anti-Kir2.1/KCNJ2 Antibody
    A Guinea Pig Polyclonal Antibody to Kir2.1 (KCNJ2) Channel
    • Product Code: APC-026-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to Kir2.1 (KCNJ2) Channel
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot
    • Target: Inward rectifier potassium channel 2, IRK1, HIRK1, LQT7, SQT3
  • Guinea pig Anti-K<sub>ir</sub>4.1 (KCNJ10) Antibody
    KO Validate'
    Guinea pig Anti-Kir4.1 (KCNJ10) Antibody
    A Guinea Pig Polyclonal Antibody to Kir4.1 (KCNJ10) Channel
    • Product Code: APC-035-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to Kir4.1 (KCNJ10) Channel
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: ATP-sensitive inward rectifier potassium channel 10, KAB-2, BIR10, BIRK1, Kir1.2, Potassium channel inwardly rectifying subfamily J member 10
  • Guinea pig Anti-K<sub>ir</sub>6.2 Antibody
    Guinea pig Anti-Kir6.2 Antibody
    A Guinea Pig Polyclonal Antibody to Kir6.2 Channel
    • Product Code: APC-020-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to Kir6.2 Channel
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Inward rectifier K+ channel Kir6.2, ATP-sensitive inward rectifier potassium channel 11, KCNJ11, BIR, TNDM3
  • Guinea pig Anti-K<sub>V</sub>1.3 (KCNA3) (extracellular) Antibody
    Guinea pig Anti-KV1.3 (KCNA3) (extracellular) Antibody
    A Guinea Pig Polyclonal Antibody to KV1.3 (KCNA3) Channel
    • Product Code: APC-101-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to KV1.3 (KCNA3) Channel
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Potassium voltage-gated channel subfamily A member 3
  • Guinea pig Anti-K<sub>V</sub>2.1 Antibody
    Guinea pig Anti-KV2.1 Antibody
    A Guinea Pig Polyclonal Antibody to KV2.1 Channel
    • Product Code: APC-012-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to KV2.1 Channel
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: KCNB1, DRK1, Delayed rectifier potassium channel 1, Potassium voltage-gated channel subfamily B member 1
  • Guinea pig Anti-K<sub>V</sub>4.2 Antibody
    Guinea pig Anti-KV4.2 Antibody
    A Guinea Pig Polyclonal Antibody to KV4.2 Channel
    • Product Code: APC-023-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to KV4.2 Channel
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Voltage-gated potassium channel subfamily D member 2, KCND2, Shal1, RK5
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