Multiplex Immunodetection

Multiplex Immunodetection

Maximize Targets, Minimize Cross-Reactivity

We offer in-house–produced and thoroughly tested antibodies conjugated to a variety of fluorophores, as well as extracellular-epitope reagents optimized for multiplex immunofluorescence, immunocolocalization, and high-parameter imaging. Guinea pig–derived antibodies are also available, providing additional species flexibility to help researchers build highly specific panels with minimal cross-reactivity. All antibodies are rigorously validated for applications including immunohistochemistry (IHC), immunocytochemistry (ICC), western blot (WB), and flow cytometry.

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FAQs

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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

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322 Results
  • Guinea pig Anti-P2X4 Receptor Antibody
    Guinea pig Anti-P2X4 Receptor Antibody
    A Guinea Pig Polyclonal Antibody to P2X4 Receptor
    • Product Code: APR-002-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to P2X4 Receptor
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot
    • Target: P2RX4, P2X purinoceptor 4, ATP receptor
  • Guinea pig Anti-P2Y12 Receptor (extracellular) Antibody
    Guinea pig Anti-P2Y12 Receptor (extracellular) Antibody
    A Guinea Pig Polyclonal Antibody to P2Y12 Receptor
    • Product Code: APR-020-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to P2Y12 Receptor
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: P2Y purinoceptor 12, P2RY12, ADP-glucose receptor, Platelet ADP receptor, HORK3
  • Guinea Pig Anti-Pan Na<sub>V</sub> Antibody
    Guinea Pig Anti-Pan NaV Antibody
    A Guinea Pig Polyclonal Antibody to Pan NaV Channel
    • Product Code: ASC-003-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to Pan NaV Channel
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot
    • Target: Pan sodium channel, SP19
  • Guinea Pig Anti-Pannexin 1 Antibody
    Guinea Pig Anti-Pannexin 1 Antibody
    A Guinea Pig Polyclonal Antibody to Pannexin 1
    • Product Code: ACC-234-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to Pannexin 1
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: PANX1, PX1, MRS1, UNQ2529
  • Guinea pig Anti-Periaxin Antibody
    Guinea pig Anti-Periaxin Antibody
    A Guinea Pig Polyclonal Antibody to Periaxin
    • Product Code: ANR-091-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to Periaxin
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: PRX, CMT4F
  • Guinea pig Anti-proBDNF Antibody
    Guinea pig Anti-proBDNF Antibody
    A Guinea Pig Polyclonal Antibody to proBDNF
    • Product Code: ANT-006-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to proBDNF
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Human, Rat
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Brain-derived neurotrophic factor precursor
  • Guinea pig Anti-proNGF Antibody
    Guinea pig Anti-proNGF Antibody
    A Guinea Pig Polyclonal Antibody to proNGF
    • Product Code: ANT-005-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to proNGF
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Nerve growth factor precursor
  • Guinea Pig Anti-PSD-95 Antibody
    Guinea Pig Anti-PSD-95 Antibody
    A Guinea Pig Polyclonal Antibody to PSD-95
    • Product Code: APZ-009-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to PSD-95
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot
    • Target: Postsynaptic density protein 95, Discs large homolog 4, DLG4, Synapse-associated protein 90, SAP-90, Tax interaction protein 15, MRD62
  • Guinea Pig Anti-SCN11A (Na<sub>V</sub>1.9) Antibody
    Guinea Pig Anti-SCN11A (NaV1.9) Antibody
    A Guinea Pig Polyclonal Antibody to SCN11A (NaV1.9) Channel
    • Product Code: ASC-017-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to SCN11A (NaV1.9) Channel
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: PN5, SNS2, NAN, Sodium channel protein type 11 subunit alpha
  • Guinea pig Anti-SCN1A (Na<sub>V</sub>1.1) Antibody
    Guinea pig Anti-SCN1A (NaV1.1) Antibody
    A Guinea Pig Polyclonal Antibody to SCN1A (NaV1.1) Channel
    • Product Code: ASC-001-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to SCN1A (NaV1.1) Channel
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot
    • Target: Brain type I sodium channel, BI, Sodium channel protein type 1 subunit α
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