Multiplex Immunodetection

Multiplex Immunodetection

Maximize Targets, Minimize Cross-Reactivity

We offer in-house–produced and thoroughly tested antibodies conjugated to a variety of fluorophores, as well as extracellular-epitope reagents optimized for multiplex immunofluorescence, immunocolocalization, and high-parameter imaging. Guinea pig–derived antibodies are also available, providing additional species flexibility to help researchers build highly specific panels with minimal cross-reactivity. All antibodies are rigorously validated for applications including immunohistochemistry (IHC), immunocytochemistry (ICC), western blot (WB), and flow cytometry.

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FAQs

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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

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322 Results
  • Anti-ASIC2a-ATTO Fluor-594 Antibody
    KO Validate'
    Anti-ASIC2a-ATTO Fluor-594 Antibody
    A Rabbit Polyclonal Antibody to ASIC2a Channel Conjugated to the Fluorescent Dye ATTO-594
    • Product Code: ASC-012-AR
    • Short Description: A Rabbit Polyclonal Antibody to ASIC2a Channel Conjugated to the Fluorescent Dye ATTO-594
    • Host: Rabbit
    • Label: ATTO 594
    • Reactivity: Human, Rat, Mouse
    • Applications: Immunofluorescence, Immunohistochemistry
    • Target: ACCN1, Acid-sensing ion channel 2, Brain sodium channel 1, BNaC1, BNC1, Amiloride-sensitive cation channel neuronal 1, Mammalian degenerin homolog, MDEG
  • Anti-AXL (extracellular)-FITC Antibody
    Anti-AXL (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to AXL (extracellular) conjugated to the fluorescent dye FITC
    • Product Code: ATR-032-F
    • Short Description: A Rabbit Polyclonal Antibody to AXL (extracellular) conjugated to the fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: AXL Receptor Tyrosine Kinase, Tyrosine-Protein Kinase Receptor UFO, AXL Oncogene, Tyro7, UFO, ARK
  • Anti-BAI1 (extracellular)-FITC Antibody
    Anti-BAI1 (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to BAI1 Conjugated to the Fluorescent Dye FITC
    • Product Code: ABR-021-F
    • Short Description: A Rabbit Polyclonal Antibody to BAI1 Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Brain-specific angiogenesis inhibitor 1, Adhesion G protein-coupled receptor B1, ADGRB1, GDAIF
  • Anti-Beta 2 Na+/K+ ATPase (extracellular)-FITC Antibody
    Anti-Beta 2 Na+/K+ ATPase (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to ?-Opioid Receptor (extracellular) conjugated to the fluorescent dye FITC
    • Product Code: ANP-012-F
    • Short Description: A Rabbit Polyclonal Antibody to ?-Opioid Receptor (extracellular) conjugated to the fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: ATP1B2, Sodium/potassium-transporting ATPase subunit beta-2, Adhesion molecule on glia, AMOG
  • Anti-C3aR1 (extracellular)-FITC Antibody
    Anti-C3aR1 (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to C3aR1 (extracellular) conjugated to the fluorescent dye FITC
    • Product Code: AAR-036-F
    • Short Description: A Rabbit Polyclonal Antibody to C3aR1 (extracellular) conjugated to the fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Complement C3a Receptor 1, C3AR, C3a Anaphylatoxin Chemotactic Receptor
  • Anti-CACNA1G (Ca<sub>V</sub>3.1)-ATTO Fluor-594 Antibody
    KO Validate'
    Anti-CACNA1G (CaV3.1)-ATTO Fluor-594 Antibody
    A Rabbit Polyclonal Antibody to CACNA1G (CaV3.1) Conjugated to the Fluorescent Dye ATTO-594
    • Product Code: ACC-021-AR
    • Short Description: A Rabbit Polyclonal Antibody to CACNA1G (CaV3.1) Conjugated to the Fluorescent Dye ATTO-594
    • Host: Rabbit
    • Label: ATTO 594
    • Reactivity: Human, Rat, Mouse
    • Applications: Immunofluorescence, Immunohistochemistry
    • Target: Voltage-dependent T-type calcium channel subunit α1G
  • Anti-CACNA2D3 (Ca<sub>V</sub>α2δ3) (extracellular)-ATTO Fluor-594 Antibody
    Anti-CACNA2D3 (CaVα2δ3) (extracellular)-ATTO Fluor-594 Antibody
    A Rabbit Polyclonal Antibody to CACNA2D3 (CaVα2δ3) Subunit Conjugated to the Fluorescent Dye ATTO-594
    • Product Code: ACC-103-AR
    • Short Description: A Rabbit Polyclonal Antibody to CACNA2D3 (CaVα2δ3) Subunit Conjugated to the Fluorescent Dye ATTO-594
    • Host: Rabbit
    • Label: ATTO 594
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Voltage-dependent calcium channel subunit alpha-2/delta-3
  • Anti-CACNA2D4 (Ca<sub>V</sub>α2δ4) (extracellular)-ATTO Fluor-594 Antibody
    Anti-CACNA2D4 (CaVα2δ4) (extracellular)-ATTO Fluor-594 Antibody
    A Rabbit Polyclonal Antibody to CACNA2D4 (CaVα2δ4) Subunit Conjugated to the Fluorescent Dye ATTO-594
    • Product Code: ACC-104-AR
    • Short Description: A Rabbit Polyclonal Antibody to CACNA2D4 (CaVα2δ4) Subunit Conjugated to the Fluorescent Dye ATTO-594
    • Host: Rabbit
    • Label: ATTO 594
    • Reactivity: Rat, Mouse
    • Applications: Live cell imaging, Immunocytochemistry, Immunofluorescence
    • Target: Voltage-dependent calcium channel subunit alpha-2/delta-4, RCD4
  • Anti-CACNB2-ATTO Fluor-594 Antibody
    Anti-CACNB2-ATTO Fluor-594 Antibody
    A Rabbit Polyclonal Antibody to CACNB2 (CaVβ2) Conjugated to the Fluorescent Dye ATTO-594
    • Product Code: ACC-105-AR
    • Short Description: A Rabbit Polyclonal Antibody to CACNB2 (CaVβ2) Conjugated to the Fluorescent Dye ATTO-594
    • Host: Rabbit
    • Label: ATTO 594
    • Reactivity: Rat, Mouse
    • Applications: Immunofluorescence, Immunohistochemistry
    • Target: CaVβ2, Voltage-dependent L-type calcium channel subunit beta-2, CACNLB2, CAB2, CACB2, Lambert-Eaton myasthenic syndrome antigen B, MYSB
  • Anti-Calcium Sensing Receptor (extracellular)-FITC Antibody
    Anti-Calcium Sensing Receptor (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to Calcium Sensing Receptor Conjugated to the Fluorescent Dye FITC
    • Product Code: ACR-004-F
    • Short Description: A Rabbit Polyclonal Antibody to Calcium Sensing Receptor Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: CaSR, Extracellular calcium-sensing receptor, Parathyroid cell calcium-sensing receptor 1, PCaR1, GPRC2A
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