Multiplex Immunodetection

Multiplex Immunodetection

Maximize Targets, Minimize Cross-Reactivity

We offer in-house–produced and thoroughly tested antibodies conjugated to a variety of fluorophores, as well as extracellular-epitope reagents optimized for multiplex immunofluorescence, immunocolocalization, and high-parameter imaging. Guinea pig–derived antibodies are also available, providing additional species flexibility to help researchers build highly specific panels with minimal cross-reactivity. All antibodies are rigorously validated for applications including immunohistochemistry (IHC), immunocytochemistry (ICC), western blot (WB), and flow cytometry.

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FAQs

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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

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322 Results
  • Guinea pig Anti-SCN2A (Na<sub>V</sub>1.2) Antibody
    Guinea pig Anti-SCN2A (NaV1.2) Antibody
    A Guinea Pig Polyclonal Antibody to SCN2A (NaV1.2) Channel
    • Product Code: ASC-002-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to SCN2A (NaV1.2) Channel
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: BII, Brain type II Na+ channel, Sodium channel protein type 2 subunit alpha
  • Guinea pig Anti-Stargazin Antibody
    Guinea pig Anti-Stargazin Antibody
    A Guinea Pig Polyclonal Antibody to Stargazin (CaVγ2 Subunit)
    • Product Code: ACC-012-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to Stargazin (CaVγ2 Subunit)
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot
    • Target: CaVγ2, Voltage-dependent calcium channel gamma-2 subunit, CACNG2, TARP gamma-2, Transmembrane AMPAR regulatory protein gamma-2, Waggler
  • Guinea Pig Anti-Synaptophysin Antibody
    Guinea Pig Anti-Synaptophysin Antibody
    A Guinea Pig Polyclonal Antibody to Synaptophysin
    • Product Code: ANR-013-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to Synaptophysin
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: Major synaptic vesicle protein p38, SYP
  • Guinea Pig Anti-Synaptotagmin-1 (SYT1) Antibody
    Guinea Pig Anti-Synaptotagmin-1 (SYT1) Antibody
    A Guinea Pig Polyclonal Antibody to Anti-Synaptotagmin-1 (SYT1)
    • Product Code: ANR-003-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to Anti-Synaptotagmin-1 (SYT1)
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: Synaptotagmin I, SytI, p65, SVP65
  • Guinea Pig Anti-SynGAP Antibody
    Guinea Pig Anti-SynGAP Antibody
    A Guinea Pig Polyclonal Antibody to SynGAP protein
    • Product Code: APZ-032-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to SynGAP protein
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: SynGAP
  • Guinea Pig Anti-TrkB (extracellular) Antibody
    Guinea Pig Anti-TrkB (extracellular) Antibody
    A Guinea Pig Polyclonal Antibody to TrkB
    • Product Code: ANT-019-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to TrkB
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: Neurotrophic tyrosine kinase receptor type 2, NTRK2, BDNF/NT-3 growth factors receptor, TrkB Tyrosine Kinase
  • Guinea Pig Anti-TRPA1 (extracellular) Antibody
    Guinea Pig Anti-TRPA1 (extracellular) Antibody
    A Guinea Pig Polyclonal Antibody to TRPA1 (extracellular)
    • Product Code: ACC-037-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to TRPA1 (extracellular)
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: Transient receptor potential cation channel subfamily A member 1, ANKTM1, Ankyrin-like with transmembrane domains protein 1, Transformation-sensitive protein p120, Wasabi Receptor
  • Guinea pig Anti-TRPC3 Antibody
    Guinea pig Anti-TRPC3 Antibody
    A Guinea Pig Polyclonal Antibody to TRPC3 Channel
    • Product Code: ACC-016-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to TRPC3 Channel
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: Short transient receptor potential channel 3, Transient receptor protein 3, TRP3
  • Guinea pig Anti-TRPC6 Antibody
    Guinea pig Anti-TRPC6 Antibody
    A Guinea Pig Polyclonal Antibody to TRPC6 Channel
    • Product Code: ACC-017-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to TRPC6 Channel
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Short transient receptor potential channel 6, Transient receptor protein 6, TRP6
  • Guinea pig Anti-TRPV1 (VR1) Antibody
    Guinea pig Anti-TRPV1 (VR1) Antibody
    A Guinea Pig Polyclonal Antibody to TRPV1 (VR1) Channel
    • Product Code: ACC-030-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to TRPV1 (VR1) Channel
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Transient receptor potential cation channel subfamily V member 1, Capsaicin receptor, Vanilloid receptor 1, OTRPC1
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