Multiplex Immunodetection

Multiplex Immunodetection

Maximize Targets, Minimize Cross-Reactivity

We offer in-house–produced and thoroughly tested antibodies conjugated to a variety of fluorophores, as well as extracellular-epitope reagents optimized for multiplex immunofluorescence, immunocolocalization, and high-parameter imaging. Guinea pig–derived antibodies are also available, providing additional species flexibility to help researchers build highly specific panels with minimal cross-reactivity. All antibodies are rigorously validated for applications including immunohistochemistry (IHC), immunocytochemistry (ICC), western blot (WB), and flow cytometry.

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FAQs

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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

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322 Results
  • Guinea pig Anti-TRPV2 (VRL1) (extracellular) Antibody
    Guinea pig Anti-TRPV2 (VRL1) (extracellular) Antibody
    A Guinea Pig Polyclonal Antibody to TRPV2 (VRL1) Channel
    • Product Code: ACC-039-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to TRPV2 (VRL1) Channel
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Transient receptor potential cation channel subfamily V member 2, Vanilloid receptor-like protein 1, VRL-1, Osm-9-like TRP channel 2, OTRPC2, Stretch-activated channel 2B, SAC2B
  • Guinea pig Anti-TRPV4 Antibody
    Guinea pig Anti-TRPV4 Antibody
    A Guinea Pig Polyclonal Antibody to TRPV4 Channel
    • Product Code: ACC-034-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to TRPV4 Channel
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Mouse
    • Applications: Western blot
    • Target: OTRPC4, VRL-2, VR-OAC, TRP12
  • Guinea Pig Anti-Vasopressin V<sub>1A</sub> Receptor (AVPR1A) Antibody
    Guinea Pig Anti-Vasopressin V1A Receptor (AVPR1A) Antibody
    A Guinea Pig Polyclonal Antibody to Vasopressin V1A Receptor (AVPR1A)
    • Product Code: AVR-010-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to Vasopressin V1A Receptor (AVPR1A)
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: V1aR, AVPR V1a, Antidiuretic hormone receptor 1a, Vascular/hepatic-type arginine vasopressin receptor
  • Guinea pig Anti-Vesicular GABA Transporter (VGAT) Antibody
    Guinea pig Anti-Vesicular GABA Transporter (VGAT) Antibody
    A Guinea Pig Polyclonal Antibody to Vesicular GABA Transporter
    • Product Code: AGT-005-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to Vesicular GABA Transporter
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: Vesicular inhibitory amino acid transporter, VIAAT, GABA and glycine transporter, rGVAT, SLC32A1
  • Guinea Pig Anti-VGLUT1 Antibody
    Guinea Pig Anti-VGLUT1 Antibody
    A Guinea Pig Polyclonal Antibody to VGLUT1
    • Product Code: AGC-035-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to VGLUT1
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: Vesicular glutamate transporter 1, Brain-specific Na+-dependent inorganic phosphate cotransporter, BNPI, SLC17A7
  • Guinea Pig Anti-VGLUT2 Antibody
    Guinea Pig Anti-VGLUT2 Antibody
    A Guinea Pig Polyclonal Antibody to Vesicular Glutamate Transporter 2
    • Product Code: AGC-036-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to Vesicular Glutamate Transporter 2
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: Vesicular glutamate transporter 2, Differentiation-associated Na+-dependent inorganic phosphate cotransporter, DNPI, SLC17A6
  • Mouse Anti-GIRK1 (K<sub>ir</sub>3.1) (extracellular) Antibody
    Mouse Anti-GIRK1 (Kir3.1) (extracellular) Antibody
    A Mouse Monoclonal Antibody to GIRK1 (Kir3.1) Channel
    • Product Code: ALM-031
    • Short Description: A Mouse Monoclonal Antibody to GIRK1 (Kir3.1) Channel
    • Host: Mouse
    • Label: Unconjugated
    • Reactivity: Human, Rat
    • Applications: Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence
    • Target: KCNJ3, G protein-activated inward rectifier potassium channel 1
  • Mouse Anti-Human Orai1 (extracellular) Antibody
    Mouse Anti-Human Orai1 (extracellular) Antibody
    A Mouse Monoclonal Antibody to Human Orai1 Channel
    • Product Code: ALM-025
    • Short Description: A Mouse Monoclonal Antibody to Human Orai1 Channel
    • Host: Mouse
    • Label: Unconjugated
    • Reactivity: Human
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence
    • Target: TMEM142A, CRACM1, Calcium release-activated calcium channel protein 1
  • Mouse Anti-Human Orai1 (extracellular)-ATTO Fluor-633 Antibody
    Mouse Anti-Human Orai1 (extracellular)-ATTO Fluor-633 Antibody
    A Mouse Monoclonal Antibody to Human Orai1 Channel Conjugated to the Fluorescent Dye ATTO-633
    • Product Code: ALM-025-FR
    • Short Description: A Mouse Monoclonal Antibody to Human Orai1 Channel Conjugated to the Fluorescent Dye ATTO-633
    • Host: Mouse
    • Label: ATTO 633
    • Reactivity: Human
    • Applications: Live cell imaging, Flow cytometry
    • Target: TMEM142A, CRACM1, Calcium release-activated calcium channel protein 1
  • Mouse Anti-Human Orai1 (extracellular)-Biotin Antibody
    Mouse Anti-Human Orai1 (extracellular)-Biotin Antibody
    A Mouse Monoclonal Antibody to Human Orai1 Channel Conjugated to Biotin
    • Product Code: ALM-025-B
    • Short Description: A Mouse Monoclonal Antibody to Human Orai1 Channel Conjugated to Biotin
    • Host: Mouse
    • Label: Biotin
    • Reactivity: Human
    • Applications: Western blot, Flow cytometry
    • Target: TMEM142A, CRACM1, Calcium release-activated calcium channel protein 1
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