Multiplex Immunodetection

Multiplex Immunodetection

Maximize Targets, Minimize Cross-Reactivity

We offer in-house–produced and thoroughly tested antibodies conjugated to a variety of fluorophores, as well as extracellular-epitope reagents optimized for multiplex immunofluorescence, immunocolocalization, and high-parameter imaging. Guinea pig–derived antibodies are also available, providing additional species flexibility to help researchers build highly specific panels with minimal cross-reactivity. All antibodies are rigorously validated for applications including immunohistochemistry (IHC), immunocytochemistry (ICC), western blot (WB), and flow cytometry.

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FAQs

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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

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322 Results
  • Anti-CXCR7 (ACKR3) (extracellular)-ATTO Fluor-488 Antibody
    Anti-CXCR7 (ACKR3) (extracellular)-ATTO Fluor-488 Antibody
    A Rabbit Polyclonal Antibody to CXCR7 Chemokine Receptor Conjugated to the Fluorescent Dye ATTO-488
    • Product Code: ACR-037-AG
    • Short Description: A Rabbit Polyclonal Antibody to CXCR7 Chemokine Receptor Conjugated to the Fluorescent Dye ATTO-488
    • Host: Rabbit
    • Label: ATTO 488
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry, Immunohistochemistry
    • Target: Atypical chemokine receptor 3, C-X-C chemokine receptor type 7, CXCR-7, Chemokine orphan receptor 1, G-protein coupled receptor RDC1 homolog, CMKOR1, GPR159
  • Anti-CysLTR1 (extracellular)-FITC Antibody
    Anti-CysLTR1 (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to CysLTR1 Conjugated to the Fluorescent Dye FITC
    • Product Code: ALR-003-F
    • Short Description: A Rabbit Polyclonal Antibody to CysLTR1 Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat
    • Applications: Flow cytometry
    • Target: CYSLT1, Cysteinyl leukotriene receptor 1, Cysteinyl leukotriene D4 receptor, LTD4 receptor, G-protein coupled receptor HG55, HMTMF81
  • Anti-D<sub>2</sub> Dopamine Receptor (extracellular)-FITC Antibody
    Anti-D2 Dopamine Receptor (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to D2 Dopamine Receptor Conjugated to the Fluorescent Dye FITC
    • Product Code: ADR-002-F
    • Short Description: A Rabbit Polyclonal Antibody to D2 Dopamine Receptor Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: DRD2, D(2) dopamine receptor
  • Anti-Dopamine Transporter (DAT) (extracellular)-FITC Antibody
    Anti-Dopamine Transporter (DAT) (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to Dopamine Transporter (DAT) Conjugated to the Fluorescent Dye FITC
    • Product Code: AMT-003-F
    • Short Description: A Rabbit Polyclonal Antibody to Dopamine Transporter (DAT) Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Sodium-dependent dopamine transporter, DA transporter, Solute carrier family 6 member 3, SLC6A3
  • Anti-EAAT1 (GLAST) (extracellular)-FITC Antibody
    Anti-EAAT1 (GLAST) (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to Excitatory Amino Acid Transporter 1 (EAAT1, GLAST) Conjugated to the Fluorescent Dye FITC
    • Product Code: AGC-021-F
    • Short Description: A Rabbit Polyclonal Antibody to Excitatory Amino Acid Transporter 1 (EAAT1, GLAST) Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Excitatory amino acid transporter 1, Sodium-dependent glutamate/aspartate transporter 1, GLAST-1, SLC1A3
  • Anti-EAAT2 (GLT-1) (extracellular)-ATTO Fluor-633 Antibody
    Anti-EAAT2 (GLT-1) (extracellular)-ATTO Fluor-633 Antibody
    A Rabbit Polyclonal Antibody to Excitatory Amino Acid Transporter 2 (EAAT2, GLT-1) Conjugated to the Fluorescent Dye ATTO-633
    • Product Code: AGC-022-FR
    • Short Description: A Rabbit Polyclonal Antibody to Excitatory Amino Acid Transporter 2 (EAAT2, GLT-1) Conjugated to the Fluorescent Dye ATTO-633
    • Host: Rabbit
    • Label: ATTO 633
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Immunofluorescence, Flow cytometry, Immunohistochemistry
    • Target: Excitatory amino acid transporter 2, Sodium-dependent glutamate/aspartate transporter 2, SLC1A2
  • Anti-EMR1 (ADGRE1) (extracellular)-FITC Antibody
    Anti-EMR1 (ADGRE1) (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to EMR1 Conjugated to the Fluorescent Dye FITC
    • Product Code: AER-051-F
    • Short Description: A Rabbit Polyclonal Antibody to EMR1 Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Flow cytometry
    • Target: EMR1 hormone receptor, EGF-like module-containing mucin-like hormone receptor-like 1, Adhesion G protein-coupled receptor E1, TM7LN3, F4/80, GPF480
  • Anti-EphA1 (extracellular)-FITC Antibody
    Anti-EphA1 (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to EphA1 (extracellular) conjugated to the fluorescent dye FITC
    • Product Code: AER-011-F
    • Short Description: A Rabbit Polyclonal Antibody to EphA1 (extracellular) conjugated to the fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Ephrin type-A receptor 1, Eph receptor A1, EPH tyrosine kinase 1, Tyrosine-protein kinase receptor EPH, EPHT1
  • Anti-GABA(A) α1 Receptor (extracellular)-ATTO Fluor-488 Antibody
    Anti-GABA(A) α1 Receptor (extracellular)-ATTO Fluor-488 Antibody
    A Rabbit Polyclonal Antibody to GABA(A) α1 Receptor Conjugated to the Fluorescent Dye ATTO-488
    • Product Code: AGA-001-AG
    • Short Description: A Rabbit Polyclonal Antibody to GABA(A) α1 Receptor Conjugated to the Fluorescent Dye ATTO-488
    • Host: Rabbit
    • Label: ATTO 488
    • Reactivity: Rat, Mouse
    • Applications: Live cell imaging, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: γ-Aminobutyric acid receptor type A subunit α1, GABRA1
  • Anti-GABA(A) α1 Receptor (extracellular)-FITC Antibody
    Anti-GABA(A) α1 Receptor (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to GABRA1 (extracellular) conjugated to the fluorescent dye FITC
    • Product Code: AGA-001-F
    • Short Description: A Rabbit Polyclonal Antibody to GABRA1 (extracellular) conjugated to the fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: γ-Aminobutyric acid receptor type A subunit α1, GABRA1
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