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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

43 Results
  • Anoctamin-5 Blocking Peptide
    Anoctamin-5 Blocking Peptide
    A Blocking Peptide for Anti-Anoctamin-5 Antibody
    • Product Code: BLP-CL015
    • Short Description: A Blocking Peptide for Anti-Anoctamin-5 Antibody
    • Applications: Western blot, Immunohistochemistry
    • Target: ANO5
  • Anoctamin-6 (extracellular) Blocking Peptide
    Anoctamin-6 (extracellular) Blocking Peptide
    A Blocking Peptide for Anti-Anoctamin-6 (extracellular) Antibody
    • Product Code: BLP-CL016
    • Short Description: A Blocking Peptide for Anti-Anoctamin-6 (extracellular) Antibody
    • Applications: Western blot, Immunohistochemistry
    • Target: ANO6
  • Anti-Anoctamin-5 Antibody
    Anti-Anoctamin-5 Antibody
    A Rabbit Polyclonal Antibody to Anoctamin-5 Channel
    • Product Code: ACL-015
    • Short Description: A Rabbit Polyclonal Antibody to Anoctamin-5 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot
    • Target: ANO5, Transmembrane protein 16E, TMEM16E, GDD1, LGMD2L
  • Anti-Anoctamin-6 (extracellular) Antibody
    Anti-Anoctamin-6 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Anoctamin-6 Channel
    • Product Code: ACL-016
    • Short Description: A Rabbit Polyclonal Antibody to Anoctamin-6 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot
    • Target: ANO6, Transmembrane protein 16F, TMEM16F, Small-conductance calcium-activated nonselective cation channel, SCAN channel
  • Anti-Anoctamin-6 (extracellular)-FITC Antibody
    Anti-Anoctamin-6 (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to Anoctamin-6 Conjugated to the Fluorescent Dye FITC
    • Product Code: ACL-016-F
    • Short Description: A Rabbit Polyclonal Antibody to Anoctamin-6 Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: ANO6, Transmembrane protein 16F, TMEM16F, Small-conductance calcium-activated nonselective cation channel, SCAN channel
  • Anti-Bestrophin-1 (extracellular) Antibody
    Anti-Bestrophin-1 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Bestrophin-1 Channel
    • Product Code: ABC-001
    • Short Description: A Rabbit Polyclonal Antibody to Bestrophin-1 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: BEST1, Vitelliform macular dystrophy protein 2, VMD2, BMD1
  • Anti-Bestrophin-2 (extracellular) Antibody
    Anti-Bestrophin-2 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Bestrophin-2 Channel
    • Product Code: ABC-002
    • Short Description: A Rabbit Polyclonal Antibody to Bestrophin-2 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: BEST2, Vitelliform macular dystrophy 2-like protein 1, VMD2L1
  • Anti-Calnexin Antibody
    Anti-Calnexin Antibody
    A Rabbit Polyclonal Antibody to Calnexin
    • Product Code: ACS-009
    • Short Description: A Rabbit Polyclonal Antibody to Calnexin
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Canx, Calx, CNX, Major histocompatibility complex class I antigen-binding protein p88, p90, IP90
  • Anti-Calnexin-ATTO Fluor-594 Antibody
    Anti-Calnexin-ATTO Fluor-594 Antibody
    A Rabbit Polyclonal Antibody to Calnexin Conjugated to the Fluorescent Dye ATTO-594
    • Product Code: ACS-009-AR
    • Short Description: A Rabbit Polyclonal Antibody to Calnexin Conjugated to the Fluorescent Dye ATTO-594
    • Host: Rabbit
    • Label: ATTO 594
    • Reactivity: Human, Rat, Mouse
    • Applications: Immunofluorescence, Immunohistochemistry
    • Target: Canx, Calx, CNX, Major histocompatibility complex class I antigen-binding protein p88, p90, IP90
  • Anti-CFTR Antibody
    KO Validate'
    Anti-CFTR Antibody
    A Rabbit Polyclonal Antibody to Cystic Fibrosis Transmembrane Conductance Regulator
    • Product Code: ACL-006
    • Short Description: A Rabbit Polyclonal Antibody to Cystic Fibrosis Transmembrane Conductance Regulator
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry
    • Target: Cystic fibrosis transmembrane conductance regulator, ATP-binding cassette sub-family C member 7, ABCC7, cAMP-dependent Cl- channel
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