Hyperpolarization-Activated Cation Channels (HCN)

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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

13 Results
  • PEX5L/TRIP8b Blocking Peptide
    PEX5L/TRIP8b Blocking Peptide
    A Blocking Peptide for Anti-PEX5L/TRIP8b Antibody
    • Product Code: BLP-PR070
    • Short Description: A Blocking Peptide for Anti-PEX5L/TRIP8b Antibody
    • Applications: Western blot, Immunohistochemistry
    • Target: PEX5L
  • HCN3 (extracellular) Blocking Peptide
    HCN3 (extracellular) Blocking Peptide
    A Blocking Peptide for Anti-HCN3 (extracellular) Antibody
    • Product Code: BLP-PC083
    • Short Description: A Blocking Peptide for Anti-HCN3 (extracellular) Antibody
    • Applications: Western blot, Immunohistochemistry
    • Target: HCN3
  • HCN3 Blocking Peptide
    HCN3 Blocking Peptide
    A Blocking Peptide for Anti-HCN3 Antibody
    • Product Code: BLP-PC057
    • Short Description: A Blocking Peptide for Anti-HCN3 Antibody
    • Applications: Western blot, Immunohistochemistry
    • Target: HCN3
  • HCN1 Blocking Peptide
    HCN1 Blocking Peptide
    A Blocking Peptide for Anti-HCN1 Antibody
    • Product Code: BLP-PC056
    • Short Description: A Blocking Peptide for Anti-HCN1 Antibody
    • Applications: Western blot, Immunohistochemistry
    • Target: HCN1
  • HCN4 Blocking Peptide
    HCN4 Blocking Peptide
    A Blocking Peptide for Anti-HCN4 Antibody
    • Product Code: BLP-PC052
    • Short Description: A Blocking Peptide for Anti-HCN4 Antibody
    • Applications: Western blot, Immunohistochemistry
    • Target: HCN4
  • HCN2 Blocking Peptide
    HCN2 Blocking Peptide
    A Blocking Peptide for Anti-HCN2 Antibody
    • Product Code: BLP-PC030
    • Short Description: A Blocking Peptide for Anti-HCN2 Antibody
    • Applications: Western blot, Immunohistochemistry
    • Target: HCN2
  • Anti-PEX5L (TRIP8b) Antibody
    KO Validate'
    Anti-PEX5L (TRIP8b) Antibody
    A Rabbit Polyclonal Antibody to PEX5L (TRIP8b)
    • Product Code: APR-070
    • Short Description: A Rabbit Polyclonal Antibody to PEX5L (TRIP8b)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: PEX5R, PEX5Rp, PEX5-related protein, PEX5-like protein, Peroxin-5-related protein, PEX2-related protein, PXR2, Peroxisome biogenesis factor 5-like, TPR-containing Rab8b-interacting protein
  • Anti-HCN3 (extracellular) Antibody
    Anti-HCN3 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to HCN3 Channel
    • Product Code: APC-083
    • Short Description: A Rabbit Polyclonal Antibody to HCN3 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence
    • Target: K+/Na+ Hyperpolarization-activated cyclic nucleotide-gated channel 3, BCNG4, Hac3
  • Anti-HCN3 Antibody
    Anti-HCN3 Antibody
    A Rabbit Polyclonal Antibody to HCN3 Channel
    • Product Code: APC-057
    • Short Description: A Rabbit Polyclonal Antibody to HCN3 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: K+/Na+ Hyperpolarization-activated cyclic nucleotide-gated channel 3, BCNG4, Hac3
  • Anti-HCN1 Antibody
    KO Validate'
    Anti-HCN1 Antibody
    A Rabbit Polyclonal Antibody to HCN1 Channel
    • Product Code: APC-056
    • Short Description: A Rabbit Polyclonal Antibody to HCN1 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Hyperpolarization-activated cyclic nucleotide-gated potassium channel 1, HAC2, Brain cyclic nucleotide-gated channel 1, BCNG1, Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1
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