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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

22 Results
  • Anti-Adenosine A<sub>2A</sub> Receptor (extracellular)-APC Antibody
    Anti-Adenosine A2A Receptor (extracellular)-APC Antibody
    A Rabbit Polyclonal Antibody to Adenosine A2A Receptor Conjugated to the Allophycocyanin (APC) fluorophore
    • Product Code: AAR-008-APC
    • Short Description: A Rabbit Polyclonal Antibody to Adenosine A2A Receptor Conjugated to the Allophycocyanin (APC) fluorophore
    • Host: Rabbit
    • Reactivity: Rat, Mouse
    • Applications: Flow cytometry
    • Target: ADORA2A, A2AAR, Adenosine A2aR
  • human BDNF-ATTO Fluor-647N
    Bioassay Tested
    human BDNF-ATTO Fluor-647N
    A fluorescent recombinant human BDNF for highly sensitive detection of TrkB/p75NTR receptors, live-cell imaging, neuronal development, and synaptic plasticity studies
    • Product Code: B-250-FRN
    • Short Description: A fluorescent recombinant human BDNF for highly sensitive detection of TrkB/p75NTR receptors, live-cell imaging, neuronal development, and synaptic plasticity studies
    • Applications: Apoptosis assay, Cell-Based Assays, Ex vivo, In vivo, Survival assay, Direct flow cytometry, Fluorescence staining, Live cell imaging, Immunofluorescence, Western blot
    • MW: 14134 Da
  • Calciseptine-ATTO Fluor-647N
    Bioassay Tested
    Calciseptine-ATTO Fluor-647N
    A fluorescent probe for highly specific imaging and detection of neuronal L-type (CaV1.2) calcium channels
    • Product Code: SPC-500-FRN
    • Short Description: A fluorescent probe for highly specific imaging and detection of neuronal L-type (CaV1.2) calcium channels
    • Applications: Electrophysiology, Direct flow cytometry, Fluorescence staining, Live cell imaging, Immunofluorescence
    • MW: ~7978 Da
  • Anti-GPR83 (extracellular)-FITC Antibody
    Anti-GPR83 (extracellular)-FITC Antibody
    A rabbit polyclonal antibody to GPR83 Conjugated to the Fluorescent Dye FITC
    • Product Code: AGR-053-F
    • Short Description: A rabbit polyclonal antibody to GPR83 Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Reactivity: Human, Rat, Mouse
    • Applications: Flow cytometry
    • Target: G-protein coupled receptor 83, G-protein coupled receptor 72, GPR72, Glucocorticoid induced receptor, GIR
  • Anti-GPR41/FFAR3 (extracellular) Antibody
    Anti-GPR41/FFAR3 (extracellular) Antibody
    A rabbit polyclonal antibody to GPR41/FFAR3 (extracellular)
    • Product Code: AFR-017
    • Short Description: A rabbit polyclonal antibody to GPR41/FFAR3 (extracellular)
    • Host: Rabbit
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: FFAR3, GPR41, Free fatty acid receptor 3, G-protein coupled receptor 41
  • Anti-P2X7 Receptor (Splice variant B) Antibody
    Anti-P2X7 Receptor (Splice variant B) Antibody
    A rabbit polyclonal antibody to P2X7 Receptor (Splice variant B)
    • Product Code: APR-107
    • Short Description: A rabbit polyclonal antibody to P2X7 Receptor (Splice variant B)
    • Host: Rabbit
    • Reactivity: Human
    • Applications: Western blot, Immunocytochemistry
    • Target: P2X purinoceptor 7, P2RX7 isoform B, P2X7-B, P2X7B
  • Anti-Endophilin-A1 Antibody
    Anti-Endophilin-A1 Antibody
    A rabbit polyclonal antibody to Endophilin-A1
    • Product Code: APZ-065
    • Short Description: A rabbit polyclonal antibody to Endophilin-A1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: SH3GL2
  • ω-Agatoxin IVA-Biotin
    Bioassay Tested
    ω-Agatoxin IVA-Biotin
    A biotinylated ω-Agatoxin IVA for imaging and sensitive detection of P/Q-Type CaV Channels and Presynaptic Neuromuscular Junctions.
    • Product Code: STA-500-B
    • Short Description: A biotinylated ω-Agatoxin IVA for imaging and sensitive detection of P/Q-Type CaV Channels and Presynaptic Neuromuscular Junctions.
    • Label: Biotin
    • Applications: Fluorescence staining, Live cell imaging, Immunofluorescence, Indirect flow cytometry
    • MW: 5710 Da
  • α-Conotoxin GI-Biotin
    Bioassay Tested
    α-Conotoxin GI-Biotin
    A biotinylated probe for imaging and sensitive detection of muscle-type nicotinic acetylcholine receptor (nAChR)
    • Product Code: STC-500-B
    • Short Description: A biotinylated probe for imaging and sensitive detection of muscle-type nicotinic acetylcholine receptor (nAChR)
    • Label: Biotin
    • Applications: Fluorescence staining, Live cell imaging, Immunofluorescence, Indirect flow cytometry
    • MW: 1946 Da
  • Osu1 Toxin-ATTO Fluor-647N
    Bioassay Tested
    Osu1 Toxin-ATTO Fluor-647N
    A fluorescent probe for imaging and sensitive detection of KV1.5 channels.
    • Product Code: STK-010-FRN
    • Short Description: A fluorescent probe for imaging and sensitive detection of KV1.5 channels.
    • Label: ATTO 647N
    • Applications: Electrophysiology, Direct flow cytometry, Fluorescence staining, Live cell imaging, Immunofluorescence
    • MW: ~8418 Da
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