KCa1.1 (BK)

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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

12 Results
  • Iberiotoxin-ATTO Fluor-647N
    Bioassay Tested
    Iberiotoxin-ATTO Fluor-647N
    A fluorescent probe for imaging and sensitive detection of KCa1.1 (BK) K+ channels
    • Product Code: STI-400-FRN
    • Short Description: A fluorescent probe for imaging and sensitive detection of KCa1.1 (BK) K+ channels
    • Label: ATTO 647N
    • Applications: Electrophysiology, Direct flow cytometry, Fluorescence staining, Live cell imaging, Immunofluorescence
    • MW: 4858 Da
  • Tertiapin-Q-ATTO Fluor-633
    Bioassay Tested
    Tertiapin-Q-ATTO Fluor-633
    A fluorescent probe for imaging and sensitive detection of Kir1.1 and GIRK potassium channels
    • Product Code: STT-170-FR
    • Short Description: A fluorescent probe for imaging and sensitive detection of Kir1.1 and GIRK potassium channels
    • Label: ATTO 633
    • Applications: Electrophysiology, Fluorescence staining, Live cell imaging, Immunofluorescence
    • MW: 3159.6 Da
  • Tertiapin-Q-ATTO Fluor-488
    Bioassay Tested
    Tertiapin-Q-ATTO Fluor-488
    A fluorescent probe for imaging and sensitive detection of Kir1.1 and GIRK potassium channels
    • Product Code: STT-170-AG
    • Short Description: A fluorescent probe for imaging and sensitive detection of Kir1.1 and GIRK potassium channels
    • Label: ATTO 488
    • Applications: Electrophysiology, Direct flow cytometry, Live cell imaging, Immunofluorescence
    • MW: 3195.5 Da
  • Tertiapin
    Bioassay Tested
    Tertiapin
    A potent blocker of Kir1.1, GIRK, and KCa1.1 (BK) potassium channels
    • Product Code: STT-250
    • Short Description: A potent blocker of Kir1.1, GIRK, and KCa1.1 (BK) potassium channels
    • Applications: Electrophysiology
    • CAS No.: 58694-52-3
    • MW: 2455 Da
  • Tertiapin-Q
    Bioassay Tested
    Tertiapin-Q
    A potent blocker of Kir1.1 and GIRK potassium channels
    • Product Code: STT-170
    • Short Description: A potent blocker of Kir1.1 and GIRK potassium channels
    • Applications: Electrophysiology
    • CAS No.: 910044-56-3
    • MW: 2452 Da
  • Slotoxin
    Bioassay Tested
    Slotoxin
    A potent and selective blocker of KCa1.1 (BK) channels
    • Product Code: STS-410
    • Short Description: A potent and selective blocker of KCa1.1 (BK) channels
    • Applications: Electrophysiology
    • CAS No.: 401470-29-9
    • MW: 4085.9 Da
  • Noxiustoxin
    Bioassay Tested
    Noxiustoxin
    A potent blocker of KV1.3 and KV1.2 K+ channels
    • Product Code: STN-340
    • Short Description: A potent blocker of KV1.3 and KV1.2 K+ channels
    • Applications: Electrophysiology
    • CAS No.: 85205-49-8
    • MW: 4195 Da
  • Kaliotoxin-1
    Bioassay Tested
    Kaliotoxin-1
    A potent blocker of KV1.1, KV1.3 and KCa1.1 (BK) channels
    • Product Code: STK-370
    • Short Description: A potent blocker of KV1.1, KV1.3 and KCa1.1 (BK) channels
    • Applications: Electrophysiology
    • CAS No.: 145199-73-1
    • MW: 4149 Da
  • Iberiotoxin
    Bioassay Tested
    Iberiotoxin
    A potent and specific blocker of KCa1.1 K+ channels
    • Product Code: STI-400
    • Short Description: A potent and specific blocker of KCa1.1 K+ channels
    • Applications: Electrophysiology
    • CAS No.: 129203-60-7
    • MW: 4230.8 Da
  • Charybdotoxin
    Bioassay Tested
    Charybdotoxin
    A potent broad-spectrum blocker of K+ channels, including KV1.2, KV1.3, KV1.6, KCa1.1 and KCa3.1 channels
    • Product Code: STC-325
    • Short Description: A potent broad-spectrum blocker of K+ channels, including KV1.2, KV1.3, KV1.6, KCa1.1 and KCa3.1 channels
    • Applications: Electrophysiology
    • CAS No.: 95751-30-7
    • MW: 4296 Da
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