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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

2041 Results
  • Anti-GLUT5 (SLC2A5) Antibody
    Anti-GLUT5 (SLC2A5) Antibody
    A Rabbit Polyclonal Antibody to Glucose Transporter 5
    • Product Code: AGT-025
    • Short Description: A Rabbit Polyclonal Antibody to Glucose Transporter 5
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Glucose transporter 5, Facilitated glucose transporter member 5, Fructose transporter
  • Anti-GLUT4-ATTO Fluor-594 Antibody
    Anti-GLUT4-ATTO Fluor-594 Antibody
    A Rabbit Polyclonal Antibody to Glucose Transporter 4 Conjugated to the Fluorescent Dye ATTO-594
    • Product Code: AGT-024-AR
    • Short Description: A Rabbit Polyclonal Antibody to Glucose Transporter 4 Conjugated to the Fluorescent Dye ATTO-594
    • Host: Rabbit
    • Label: ATTO 594
    • Reactivity: Human, Rat, Mouse
    • Applications: Immunofluorescence, Immunohistochemistry
    • Target: Glucose transporter 4, Facilitated glucose transporter member 4, Insulin-responsive glucose transporter type 4, SLC2A4
  • Anti-GLUT4 Antibody
    Anti-GLUT4 Antibody
    A Rabbit Polyclonal Antibody to Glucose Transporter 4
    • Product Code: AGT-024
    • Short Description: A Rabbit Polyclonal Antibody to Glucose Transporter 4
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Glucose transporter 4, Facilitated glucose transporter member 4, Insulin-responsive glucose transporter type 4, SLC2A4
  • Anti-GLUT3 (extracellular)-FITC Antibody
    Anti-GLUT3 (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to Glucose Transporter 3 Conjugated to the Fluorescent Dye FITC
    • Product Code: AGT-023-F
    • Short Description: A Rabbit Polyclonal Antibody to Glucose Transporter 3 Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Rat, Mouse
    • Applications: Flow cytometry
    • Target: Glucose transporter 3, Facilitated glucose transporter member 3, SLC2A3
  • Anti-GLUT3 (extracellular) Antibody
    Anti-GLUT3 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Glucose Transporter 3
    • Product Code: AGT-023
    • Short Description: A Rabbit Polyclonal Antibody to Glucose Transporter 3
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Indirect flow cytometry, Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Glucose transporter 3, Facilitated glucose transporter member 3, SLC2A3
  • Anti-GLUT2 (SLC2A2)-ATTO Fluor-488 Antibody
    Anti-GLUT2 (SLC2A2)-ATTO Fluor-488 Antibody
    A Rabbit Polyclonal Antibody to Glucose Transporter 2 Conjugated to the Fluorescent Dye ATTO-488
    • Product Code: AGT-022-AG
    • Short Description: A Rabbit Polyclonal Antibody to Glucose Transporter 2 Conjugated to the Fluorescent Dye ATTO-488
    • Host: Rabbit
    • Label: ATTO 488
    • Reactivity: Rat, Mouse
    • Applications: Immunofluorescence, Immunohistochemistry
    • Target: Glucose transporter 2, Facilitated glucose transporter member 2
  • Anti-GLUT2 (SLC2A2) Antibody
    Anti-GLUT2 (SLC2A2) Antibody
    A Rabbit Polyclonal Antibody to Glucose Transporter 2
    • Product Code: AGT-022
    • Short Description: A Rabbit Polyclonal Antibody to Glucose Transporter 2
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Glucose transporter 2, Facilitated glucose transporter member 2
  • Anti-GLUT1 Antibody
    Anti-GLUT1 Antibody
    A Rabbit Polyclonal Antibody to Glucose Transporter 1
    • Product Code: AGT-021
    • Short Description: A Rabbit Polyclonal Antibody to Glucose Transporter 1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Glucose transporter 1, Facilitated glucose transporter member 1, SLC2A1
  • Anti-SLC6A8 Antibody
    Anti-SLC6A8 Antibody
    A Rabbit Polyclonal Antibody to Creatine Transporter
    • Product Code: AGT-008
    • Short Description: A Rabbit Polyclonal Antibody to Creatine Transporter
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Creatine transporter, Sodium- and chloride-dependent creatine transporter 1, CT1, CRT, CHOT1
  • Anti-Taurine Transporter (SLC6A6) Antibody
    Anti-Taurine Transporter (SLC6A6) Antibody
    A Rabbit Polyclonal Antibody to Taurine Transporter (SLC6A6)
    • Product Code: AGT-006
    • Short Description: A Rabbit Polyclonal Antibody to Taurine Transporter (SLC6A6)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot
    • Target: Sodium- and chloride-dependent taurine transporter, TauT
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