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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

2041 Results
  • Anti-GPR65 (TDAG8) (extracellular)-FITC Antibody
    Anti-GPR65 (TDAG8) (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to GPR65 (TDAG8) Conjugated to the Fluorescent Dye FITC
    • Product Code: AGR-043-F
    • Short Description: A Rabbit Polyclonal Antibody to GPR65 (TDAG8) Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: G-protein coupled receptor 65, T-cell death-associated gene 8 protein, Psychosine receptor
  • Anti-GPR65 (TDAG8) (extracellular) Antibody
    Anti-GPR65 (TDAG8) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to GPR65 (TDAG8)
    • Product Code: AGR-043
    • Short Description: A Rabbit Polyclonal Antibody to GPR65 (TDAG8)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunofluorescence, Immunohistochemistry
    • Target: G-protein coupled receptor 65, T-cell death-associated gene 8 protein, Psychosine receptor
  • Anti-GPR68 (OGR1) (extracellular)-FITC Antibody
    Anti-GPR68 (OGR1) (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to GPR68 Conjugated to the Fluorescent Dye FITC
    • Product Code: AGR-042-F
    • Short Description: A Rabbit Polyclonal Antibody to GPR68 Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Acid-sensing G-protein coupled receptor 68, Ovarian cancer G-protein coupled receptor 1, Sphingosylphosphorylcholine receptor
  • Anti-GPR68 (OGR1) (extracellular)-APC Antibody
    Anti-GPR68 (OGR1) (extracellular)-APC Antibody
    A Rabbit Polyclonal Antibody to GPR68 (extracellular) conjugated to the fluorescent dye Allophycocyanin (APC)
    • Product Code: AGR-042-APC
    • Short Description: A Rabbit Polyclonal Antibody to GPR68 (extracellular) conjugated to the fluorescent dye Allophycocyanin (APC)
    • Host: Rabbit
    • Label: APC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Acid-sensing G-protein coupled receptor 68, Ovarian cancer G-protein coupled receptor 1, Sphingosylphosphorylcholine receptor, AI2A6
  • Anti-GPR68 (OGR1) (extracellular) Antibody
    Anti-GPR68 (OGR1) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to GPR68 (OGR1)
    • Product Code: AGR-042
    • Short Description: A Rabbit Polyclonal Antibody to GPR68 (OGR1)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot
    • Target: Acid-sensing G-protein coupled receptor 68, Ovarian cancer G-protein coupled receptor 1, Sphingosylphosphorylcholine receptor
  • Anti-GPR4 (extracellular)-FITC Antibody
    Anti-GPR4 (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to GPR4 (extracellular) conjugated to the fluorescent dye FITC
    • Product Code: AGR-041-F
    • Short Description: A Rabbit Polyclonal Antibody to GPR4 (extracellular) conjugated to the fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Acid-sensing G-protein coupled receptor 4, GPR19
  • Anti-GPR4 (extracellular) Antibody
    Anti-GPR4 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to GPR4
    • Product Code: AGR-041
    • Short Description: A Rabbit Polyclonal Antibody to GPR4
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: Acid-sensing G-protein coupled receptor 4, GPR19
  • Anti-GPR132 (extracellular) Antibody
    Anti-GPR132 (extracellular) Antibody
    A Rabbit Polyclonal antibody to GPR132 (extracellular)
    • Product Code: AGR-036
    • Short Description: A Rabbit Polyclonal antibody to GPR132 (extracellular)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot
    • Target: G Protein-Coupled Receptor 132, G2 Accumulation Protein, G2A
  • Anti-GPR119 Antibody
    Anti-GPR119 Antibody
    A Rabbit Polyclonal Antibody to Glucose-Dependent Insulinotropic Receptor (GPR119)
    • Product Code: AGR-032
    • Short Description: A Rabbit Polyclonal Antibody to Glucose-Dependent Insulinotropic Receptor (GPR119)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot
    • Target: Glucose-dependent insulinotropic receptor, GPCR2
  • Anti-Ghrelin Receptor (GHSR) (extracellular)-FITC Antibody
    Anti-Ghrelin Receptor (GHSR) (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to Ghrelin Receptor (GHSR) Conjugated to the Fluorescent Dye FITC
    • Product Code: AGR-031-F
    • Short Description: A Rabbit Polyclonal Antibody to Ghrelin Receptor (GHSR) Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Flow cytometry
    • Target: Growth hormone secretagogue receptor type 1, GHS-R, GH-releasing peptide receptor, GHRP
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