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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

2041 Results
  • Anti-GRID2 (extracellular) Antibody
    Anti-GRID2 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Glutamate Receptor δ2 (GRID2)
    • Product Code: AGC-039
    • Short Description: A Rabbit Polyclonal Antibody to Glutamate Receptor δ2 (GRID2)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Ionotropic glutamate receptor δ2, GluR delta-2 subunit, GluD2
  • Anti-GRID1 (extracellular) Antibody
    Anti-GRID1 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Glutamate Receptor δ1 (GRID1)
    • Product Code: AGC-038
    • Short Description: A Rabbit Polyclonal Antibody to Glutamate Receptor δ1 (GRID1)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Ionotropic glutamate receptor δ1, GluR delta-1 subunit, GluD1
  • Guinea Pig Anti-VGLUT2 Antibody
    Guinea Pig Anti-VGLUT2 Antibody
    A Guinea Pig Polyclonal Antibody to Vesicular Glutamate Transporter 2
    • Product Code: AGC-036-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to Vesicular Glutamate Transporter 2
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: Vesicular glutamate transporter 2, Differentiation-associated Na+-dependent inorganic phosphate cotransporter, DNPI, SLC17A6
  • Anti-VGLUT2-ATTO Fluor-594 Antibody
    Anti-VGLUT2-ATTO Fluor-594 Antibody
    A Rabbit Polyclonal Antibody to Vesicular Glutamate Transporter 2 Conjugated to the Fluorescent Dye ATTO-594
    • Product Code: AGC-036-AR
    • Short Description: A Rabbit Polyclonal Antibody to Vesicular Glutamate Transporter 2 Conjugated to the Fluorescent Dye ATTO-594
    • Host: Rabbit
    • Label: ATTO 594
    • Reactivity: Human, Rat, Mouse
    • Applications: Immunofluorescence, Immunohistochemistry
    • Target: Vesicular glutamate transporter 2, Differentiation-associated Na+-dependent inorganic phosphate cotransporter, DNPI, SLC17A6
  • Anti-VGLUT2 Antibody
    Anti-VGLUT2 Antibody
    A Rabbit Polyclonal Antibody to Vesicular Glutamate Transporter 2
    • Product Code: AGC-036
    • Short Description: A Rabbit Polyclonal Antibody to Vesicular Glutamate Transporter 2
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Vesicular glutamate transporter 2, Differentiation-associated Na+-dependent inorganic phosphate cotransporter, DNPI, SLC17A6
  • Guinea Pig Anti-VGLUT1 Antibody
    Guinea Pig Anti-VGLUT1 Antibody
    A Guinea Pig Polyclonal Antibody to VGLUT1
    • Product Code: AGC-035-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to VGLUT1
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: Vesicular glutamate transporter 1, Brain-specific Na+-dependent inorganic phosphate cotransporter, BNPI, SLC17A7
  • Anti-VGLUT1 Antibody
    Anti-VGLUT1 Antibody
    A Rabbit Polyclonal Antibody to Vesicular Glutamate Transporter 1
    • Product Code: AGC-035
    • Short Description: A Rabbit Polyclonal Antibody to Vesicular Glutamate Transporter 1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Vesicular glutamate transporter 1, Brain-specific Na+-dependent inorganic phosphate cotransporter, BNPI, SLC17A7
  • Anti-NMDAR3A (GRIN3A) (extracellular) Antibody
    Anti-NMDAR3A (GRIN3A) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to NMDA Receptor 3A (GRIN3A)
    • Product Code: AGC-030
    • Short Description: A Rabbit Polyclonal Antibody to NMDA Receptor 3A (GRIN3A)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot
    • Target: NR3A, NMDAR-L, GluN3A, Ionotropic glutamate receptor NMDA 3A, N-methyl-D-aspartate subunit 3A
  • Anti-mGluR8 (extracellular) Antibody
    Anti-mGluR8 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Metabotropic Glutamate Receptor 8
    • Product Code: AGC-028
    • Short Description: A Rabbit Polyclonal Antibody to Metabotropic Glutamate Receptor 8
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Metabotropic glutamate receptor 8, GRM8, mGlu8, GPRC1H
  • Anti-EAAT3 (EAAC1) Antibody
    Anti-EAAT3 (EAAC1) Antibody
    A Rabbit Polyclonal Antibody to Excitatory Amino Acid Transporter 3 (EAAT3, EAAC1)
    • Product Code: AGC-023
    • Short Description: A Rabbit Polyclonal Antibody to Excitatory Amino Acid Transporter 3 (EAAT3, EAAC1)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Excitatory amino acid transporter 3, Excitatory amino-acid carrier 1, Sodium-dependent glutamate/aspartate transporter 3, SLC1A1
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