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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

2041 Results
  • Anti-Human FPR1 (extracellular) Antibody
    Anti-Human FPR1 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Human FPR1
    • Product Code: AFR-001
    • Short Description: A Rabbit Polyclonal Antibody to Human FPR1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot
    • Target: N-formyl peptide receptor 1, fMLP receptor
  • Guinea Pig Anti-GFAP Antibody
    Guinea Pig Anti-GFAP Antibody
    A Guinea Pig Polyclonal Antibody to Glial Fibrillary Acidic Protein
    • Product Code: AFP-001-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to Glial Fibrillary Acidic Protein
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: Glial fibrillary acidic protein
  • Anti-GFAP-ATTO Fluor-647N Antibody
    Anti-GFAP-ATTO Fluor-647N Antibody
    A Rabbit Polyclonal Antibody to GFAP conjugated to the fluorescent dye ATTO Fluor-647N
    • Product Code: AFP-001-FRN
    • Short Description: A Rabbit Polyclonal Antibody to GFAP conjugated to the fluorescent dye ATTO Fluor-647N
    • Host: Rabbit
    • Label: ATTO 647N
    • Reactivity: Rat, Mouse
    • Applications: Immunocytochemistry, Immunohistochemistry
    • Target: Glial fibrillary acidic protein
  • Anti-GFAP Antibody
    Anti-GFAP Antibody
    A Rabbit Polyclonal Antibody to Glial Fibrillary Acidic Protein
    • Product Code: AFP-001
    • Short Description: A Rabbit Polyclonal Antibody to Glial Fibrillary Acidic Protein
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Glial fibrillary acidic protein
  • Anti-Human CD97/ADGRE5 (extracellular) Antibody
    Anti-Human CD97/ADGRE5 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to CD97/ADGRE5
    • Product Code: AER-055
    • Short Description: A Rabbit Polyclonal Antibody to CD97/ADGRE5
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human
    • Applications: Indirect flow cytometry, Western blot
    • Target: Adhesion G protein-coupled receptor E5, Leukocyte antigen CD97, Seven-transmembrane heterodimeric receptor associated with inflammation
  • Guinea pig Anti-EMR1 (ADGRE1) (extracellular) Antibody
    Guinea pig Anti-EMR1 (ADGRE1) (extracellular) Antibody
    A Guinea Pig Polyclonal Antibody to EMR1
    • Product Code: AER-051-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to EMR1
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot
    • Target: MR1 hormone receptor, EGF-like module-containing mucin-like hormone receptor-like 1, Adhesion G protein-coupled receptor E1, TM7LN3, F4/80, GPF480
  • Anti-EMR1 (ADGRE1) (extracellular)-FITC Antibody
    Anti-EMR1 (ADGRE1) (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to EMR1 Conjugated to the Fluorescent Dye FITC
    • Product Code: AER-051-F
    • Short Description: A Rabbit Polyclonal Antibody to EMR1 Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Flow cytometry
    • Target: EMR1 hormone receptor, EGF-like module-containing mucin-like hormone receptor-like 1, Adhesion G protein-coupled receptor E1, TM7LN3, F4/80, GPF480
  • Anti-EMR1 (ADGRE1) (extracellular) Antibody
    Anti-EMR1 (ADGRE1) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to EMR1
    • Product Code: AER-051
    • Short Description: A Rabbit Polyclonal Antibody to EMR1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence
    • Target: EMR1 hormone receptor, EGF-like module-containing mucin-like hormone receptor-like 1, Adhesion G protein-coupled receptor E1, TM7LN3, F4/80, GPF480
  • Anti-GPER (GPR30) Antibody
    Anti-GPER (GPR30) Antibody
    A Rabbit Polyclonal Antibody to G-Protein Coupled Estrogen Receptor 1
    • Product Code: AER-050
    • Short Description: A Rabbit Polyclonal Antibody to G-Protein Coupled Estrogen Receptor 1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: G-protein coupled estrogen receptor 1, GPER1, GPR41, Chemoattractant receptor-like 2, Cmkrl2
  • Anti-GPER1/GPR30 (extracellular)-FITC Antibody
    Anti-GPER1/GPR30 (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to GPER1 Conjugated to the Fluorescent Dye FITC
    • Product Code: AER-049-F
    • Short Description: A Rabbit Polyclonal Antibody to GPER1 Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Flow cytometry
    • Target: G Protein-Coupled Estrogen Receptor 1, G Protein-Coupled Receptor 30, Membrane Estrogen Receptor, Chemoattractant Receptor-Like 2, GPER
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