Inflammation

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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

2045 Results
  • Anti-Nucleobindin-2/Nesfatin-1 Antibody
    Anti-Nucleobindin-2/Nesfatin-1 Antibody
    A Rabbit Polyclonal Antibody to Nucleobindin-2/Nesfatin-1
    • Product Code: ACS-011
    • Short Description: A Rabbit Polyclonal Antibody to Nucleobindin-2/Nesfatin-1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: NUCB2, NEFA, Prepronesfatin, DNA-binding protein NEFA, Gastric cancer antigen Zg4, HEL-S-109
  • Guinea Pig Anti-IBA1/AIF1 Antibody
    Guinea Pig Anti-IBA1/AIF1 Antibody
    A Guinea Pig Polyclonal Antibody to IBA1/AIF1
    • Product Code: ACS-010-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to IBA1/AIF1
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: Ionized calcium-binding adapter molecule 1, Allograft Inflammatory Factor 1, MRF-1, Microglia response factor
  • Anti-IBA1/AIF1 Antibody
    Anti-IBA1/AIF1 Antibody
    A Rabbit Polyclonal Antibody to IBA1/AIF1
    • Product Code: ACS-010
    • Short Description: A Rabbit Polyclonal Antibody to IBA1/AIF1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Ionized calcium-binding adapter molecule 1, Allograft Inflammatory Factor 1, MRF-1, Microglia response factor
  • Anti-Calnexin-ATTO Fluor-594 Antibody
    Anti-Calnexin-ATTO Fluor-594 Antibody
    A Rabbit Polyclonal Antibody to Calnexin Conjugated to the Fluorescent Dye ATTO-594
    • Product Code: ACS-009-AR
    • Short Description: A Rabbit Polyclonal Antibody to Calnexin Conjugated to the Fluorescent Dye ATTO-594
    • Host: Rabbit
    • Label: ATTO 594
    • Reactivity: Human, Rat, Mouse
    • Applications: Immunofluorescence, Immunohistochemistry
    • Target: Canx, Calx, CNX, Major histocompatibility complex class I antigen-binding protein p88, p90, IP90
  • Anti-Calnexin Antibody
    Anti-Calnexin Antibody
    A Rabbit Polyclonal Antibody to Calnexin
    • Product Code: ACS-009
    • Short Description: A Rabbit Polyclonal Antibody to Calnexin
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Canx, Calx, CNX, Major histocompatibility complex class I antigen-binding protein p88, p90, IP90
  • Anti-NCS1-ATTO Fluor-594 Antibody
    Anti-NCS1-ATTO Fluor-594 Antibody
    A Rabbit Polyclonal Antibody to Neuronal Calcium Sensor 1 Conjugated to the Fluorescent Dye ATTO-594
    • Product Code: ACS-001-AR
    • Short Description: A Rabbit Polyclonal Antibody to Neuronal Calcium Sensor 1 Conjugated to the Fluorescent Dye ATTO-594
    • Host: Rabbit
    • Label: ATTO 594
    • Reactivity: Human, Rat, Mouse
    • Applications: Immunofluorescence, Immunohistochemistry
    • Target: Neuronal calcium sensor 1, Frequenin homolog, Frequenin-like protein, FREQ, FLUP
  • Anti-NCS1 Antibody
    Anti-NCS1 Antibody
    A Rabbit Polyclonal Antibody to Neuronal Calcium Sensor 1
    • Product Code: ACS-001
    • Short Description: A Rabbit Polyclonal Antibody to Neuronal Calcium Sensor 1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: Neuronal calcium sensor 1, Frequenin homolog, Frequenin-like protein, FREQ, FLUP
  • Anti-GPR55 (extracellular)-FITC Antibody
    Anti-GPR55 (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to GPR55 Conjugated to the Fluorescent Dye FITC
    • Product Code: ACR-062-F
    • Short Description: A Rabbit Polyclonal Antibody to GPR55 Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: G-Protein Coupled Receptor 55
  • Anti-GPR55 (extracellular) Antibody
    Anti-GPR55 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to GPR55
    • Product Code: ACR-062
    • Short Description: A Rabbit Polyclonal Antibody to GPR55
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Indirect flow cytometry, Western blot, Immunohistochemistry
    • Target: G-Protein Coupled Receptor 55
  • Anti-Human GPR55 (extracellular) Antibody
    Anti-Human GPR55 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to GPR55
    • Product Code: ACR-061
    • Short Description: A Rabbit Polyclonal Antibody to GPR55
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human
    • Applications: Indirect flow cytometry, Western blot
    • Target: G-protein coupled receptor 55
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