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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

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2041 Results
  • Anti-CCKBR (extracellular) Antibody
    Anti-CCKBR (extracellular) Antibody
    A Rabbit Polyclonal Antibody to the Cholecystokinin B Receptor
    • Product Code: ACR-042
    • Short Description: A Rabbit Polyclonal Antibody to the Cholecystokinin B Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: Cholecystokinin B receptor, Gastrin receptor, CCK2R, GASR
  • Anti-CXCR7 (ACKR3) (extracellular)-ATTO Fluor-488 Antibody
    Anti-CXCR7 (ACKR3) (extracellular)-ATTO Fluor-488 Antibody
    A Rabbit Polyclonal Antibody to CXCR7 Chemokine Receptor Conjugated to the Fluorescent Dye ATTO-488
    • Product Code: ACR-037-AG
    • Short Description: A Rabbit Polyclonal Antibody to CXCR7 Chemokine Receptor Conjugated to the Fluorescent Dye ATTO-488
    • Host: Rabbit
    • Label: ATTO 488
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry, Immunohistochemistry
    • Target: Atypical chemokine receptor 3, C-X-C chemokine receptor type 7, CXCR-7, Chemokine orphan receptor 1, G-protein coupled receptor RDC1 homolog, CMKOR1, GPR159
  • Anti-CXCR7 (ACKR3) (extracellular) Antibody
    Anti-CXCR7 (ACKR3) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to CXCR7 Chemokine Receptor
    • Product Code: ACR-037
    • Short Description: A Rabbit Polyclonal Antibody to CXCR7 Chemokine Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot
    • Target: Atypical chemokine receptor 3, C-X-C chemokine receptor type 7, CXCR-7, Chemokine orphan receptor 1, G-protein coupled receptor RDC1 homolog, CMKOR1, GPR159
  • Anti-Rat CMKLR1 (extracellular) Antibody
    Anti-Rat CMKLR1 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Chemerin Receptor 1
    • Product Code: ACR-033
    • Short Description: A Rabbit Polyclonal Antibody to Chemerin Receptor 1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: Chemokine-like receptor 1, Cmklr1, Chemerin1, Gpcr27, G-protein coupled receptor ChemR23, CHEMERINR, G-protein coupled chemoattractant-like receptor, G-protein coupled receptor DEZ
  • Anti-CCR9 (extracellular) Antibody
    Anti-CCR9 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to CCR9 Chemokine Receptor
    • Product Code: ACR-029
    • Short Description: A Rabbit Polyclonal Antibody to CCR9 Chemokine Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot
    • Target: C-C chemokine receptor type 9, CC-CKR-9, G-protein coupled receptor 28, GPR28, GPR-9-6, CDw199
  • Anti-CCR7 (extracellular) Antibody
    Anti-CCR7 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to CCR7 Chemokine Receptor
    • Product Code: ACR-027
    • Short Description: A Rabbit Polyclonal Antibody to CCR7 Chemokine Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: C-C chemokine receptor type 7, C-C CKR-7, Epstein-Barr virus-induced G-protein coupled receptor 1, EBI1, CD197
  • Anti-Human CCR5 (extracellular) Antibody
    Anti-Human CCR5 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to CCR5 Chemokine Receptor
    • Product Code: ACR-025
    • Short Description: A Rabbit Polyclonal Antibody to CCR5 Chemokine Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot
    • Target: C-C chemokine receptor type 5, C-C CKR-5, CHEMR13, HIV-1 fusion coreceptor, CD195
  • Anti-Human CCR3 (extracellular) Antibody
    Anti-Human CCR3 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to CCR3 Chemokine Receptor
    • Product Code: ACR-023
    • Short Description: A Rabbit Polyclonal Antibody to CCR3 Chemokine Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot
    • Target: C-C chemokine receptor type 3, Eosinophil eotaxin receptor, CKR3, C-C CKR-3, CMKBR3, B-chemokine receptor, CD193 antigen
  • Anti-Human CCR2 (extracellular) Antibody
    Anti-Human CCR2 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to CCR2 Receptor
    • Product Code: ACR-022
    • Short Description: A Rabbit Polyclonal Antibody to CCR2 Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot
    • Target: C-C chemokine receptor type 2, Monocyte chemoattractant protein 1 receptor, MCP-1 receptor, CC-CKR-2, CD192
  • Anti-Human CXCR5 (extracellular) Antibody
    Anti-Human CXCR5 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to CXCR5 Chemokine Receptor
    • Product Code: ACR-015
    • Short Description: A Rabbit Polyclonal Antibody to CXCR5 Chemokine Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence
    • Target: C-X-C chemokine receptor type 5, Burkitt lymphoma receptor 1, BLR1, Monocyte-derived receptor 15, MDR15, CD185
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