Inflammation

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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.
  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

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2041 Results
  • Anti-Anoctamin-6 (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to Anoctamin-6 Conjugated to the Fluorescent Dye FITC
    • Product Code: ACL-016-F
    • Short Description: A Rabbit Polyclonal Antibody to Anoctamin-6 Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: ANO6, Transmembrane protein 16F, TMEM16F, Small-conductance calcium-activated nonselective cation channel, SCAN channel
  • Anti-Anoctamin-6 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Anoctamin-6 Channel
    • Product Code: ACL-016
    • Short Description: A Rabbit Polyclonal Antibody to Anoctamin-6 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot
    • Target: ANO6, Transmembrane protein 16F, TMEM16F, Small-conductance calcium-activated nonselective cation channel, SCAN channel
  • Anti-TMEM16A (ANO1) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Anoctamin-1 (TMEM16A) Channel
    • Product Code: ACL-011
    • Short Description: A Rabbit Polyclonal Antibody to Anoctamin-1 (TMEM16A) Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Anoctamin-1, Transmembrane protein 16A, DOG-1, ORAOV2, TAOS2
  • Anti-CFTR Antibody
    A Rabbit Polyclonal Antibody to Cystic Fibrosis Transmembrane Conductance Regulator
    • Product Code: ACL-006
    • Short Description: A Rabbit Polyclonal Antibody to Cystic Fibrosis Transmembrane Conductance Regulator
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry
    • Target: Cystic fibrosis transmembrane conductance regulator, ATP-binding cassette sub-family C member 7, ABCC7, cAMP-dependent Cl- channel
  • Anti-Human TRPV1 (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody Human TRPV1 (extracellular) conjugated to the fluorescent dye FITC
    • Product Code: ACC-334-F
    • Short Description: A Rabbit Polyclonal Antibody Human TRPV1 (extracellular) conjugated to the fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human
    • Applications: Live cell imaging, Flow cytometry
    • Target: Transient receptor potential cation channel subfamily V member 1, Capsaicin receptor, Vanilloid receptor 1, VR1, OTRPC1
  • Anti-Human TRPV1 (extracellular)-APC Antibody
    A Rabbit Polyclonal Antibody to Human TRPV1 (extracellular) conjugated to the fluorescent dye Allophycocyanin (APC)
    • Product Code: ACC-334-APC
    • Short Description: A Rabbit Polyclonal Antibody to Human TRPV1 (extracellular) conjugated to the fluorescent dye Allophycocyanin (APC)
    • Host: Rabbit
    • Label: APC
    • Reactivity: Human
    • Applications: Flow cytometry
    • Target: Transient receptor potential cation channel subfamily V member 1, Capsaicin receptor, Vanilloid receptor 1, VR1, OTRPC1
  • Anti-Human TRPV1 (extracellular) Antibody
    A Rabbit Polyclonal antibody to Human TRPV1 (extracellular)
    • Product Code: ACC-334
    • Short Description: A Rabbit Polyclonal antibody to Human TRPV1 (extracellular)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot
    • Target: Transient receptor potential cation channel subfamily V member 1, Capsaicin receptor, Vanilloid receptor 1, VR1, OTRPC1
  • Anti-MCU Antibody
    A Rabbit Polyclonal Antibody to Mitochondrial Calcium Uniporter
    • Product Code: ACC-328
    • Short Description: A Rabbit Polyclonal Antibody to Mitochondrial Calcium Uniporter
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: Mitochondrial calcium uniporter, HsMCU, Coiled-coil domain-containing protein 109A, Ccdc109a, C10orf42
  • Anti-CRACR2A (EFCAB4B) Antibody
    A Rabbit Polyclonal Antibody to CRAC Channel Regulator 2A
    • Product Code: ACC-324
    • Short Description: A Rabbit Polyclonal Antibody to CRAC Channel Regulator 2A
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Mouse
    • Applications: Western blot
    • Target: CRAC channel regulator 2A, Calcium release-activated calcium channel regulator 2A, EF-hand calcium-binding domain-containing protein 4B, EFC4B
  • Anti-MICU1 Antibody
    A Rabbit Polyclonal Antibody to Mitochondrial Calcium Uptake Protein 1 (CBARA1)
    • Product Code: ACC-322
    • Short Description: A Rabbit Polyclonal Antibody to Mitochondrial Calcium Uptake Protein 1 (CBARA1)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot
    • Target: Mitochondrial calcium uptake protein 1, Calcium-binding atopy-related autoantigen 1 homolog, ara CALC, CBARA1, MPXPS
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