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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

2041 Results
  • Anti-STIM1 (extracellular) Antibody
    KO Validate'
    Anti-STIM1 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to STIM1
    • Product Code: ACC-063
    • Short Description: A Rabbit Polyclonal Antibody to STIM1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry
    • Target: Stromal interaction molecule 1, GOK
  • Anti-Orai1 (extracellular)-FITC Antibody
    Anti-Orai1 (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to Orai1 (extracellular) conjugated to the fluorescent dye FITC.
    • Product Code: ACC-062-F
    • Short Description: A Rabbit Polyclonal Antibody to Orai1 (extracellular) conjugated to the fluorescent dye FITC.
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: TMEM142A, Calcium release-activated calcium channel protein 1, CRACM1
  • Anti-Orai1 (extracellular)-ATTO Fluor-488 Antibody
    Anti-Orai1 (extracellular)-ATTO Fluor-488 Antibody
    A Rabbit Polyclonal Antibody to Orai1 Channel Conjugated to the Fluorescent Dye ATTO-488
    • Product Code: ACC-062-AG
    • Short Description: A Rabbit Polyclonal Antibody to Orai1 Channel Conjugated to the Fluorescent Dye ATTO-488
    • Host: Rabbit
    • Label: ATTO 488
    • Reactivity: Rat, Mouse
    • Applications: Immunocytochemistry, Immunofluorescence
    • Target: TMEM142A, CRACM1, Calcium release-activated calcium channel protein 1
  • Anti-Orai1 (extracellular) Antibody
    KO Validate'
    Anti-Orai1 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Orai1 Channel
    • Product Code: ACC-062
    • Short Description: A Rabbit Polyclonal Antibody to Orai1 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: TMEM142A, CRACM1, Calcium release-activated calcium channel protein 1
  • Anti-Orai2 Antibody
    Anti-Orai2 Antibody
    A Rabbit Polyclonal Antibody to Orai2 Channel
    • Product Code: ACC-061
    • Short Description: A Rabbit Polyclonal Antibody to Orai2 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: TMEM142B
  • Anti-Human Orai1 (extracellular)-FITC Antibody
    Anti-Human Orai1 (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to Human Orai1 Channel Conjugated to the Fluorescent Dye FITC
    • Product Code: ACC-060-F
    • Short Description: A Rabbit Polyclonal Antibody to Human Orai1 Channel Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human
    • Applications: Flow cytometry
    • Target: TMEM142A, CRACM1, Calcium release-activated calcium channel protein 1
  • Anti-Human Orai1 (extracellular) Antibody
    KO Validate'
    Anti-Human Orai1 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Human Orai1 Channel
    • Product Code: ACC-060
    • Short Description: A Rabbit Polyclonal Antibody to Human Orai1 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: TMEM142A, CRACM1, Calcium release-activated calcium channel protein 1
  • Anti-TRPP1 (PKD2) Antibody
    Anti-TRPP1 (PKD2) Antibody
    A Rabbit Polyclonal Antibody to TRPP1 (PKD2) Channel
    • Product Code: ACC-052
    • Short Description: A Rabbit Polyclonal Antibody to TRPP1 (PKD2) Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Polycystin-2, Polycystic kidney disease 2 protein, PC2
  • Anti-TRPM3 (extracellular) Antibody
    Anti-TRPM3 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to TRPM3 Channel
    • Product Code: ACC-050
    • Short Description: A Rabbit Polyclonal Antibody to TRPM3 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Transient receptor potential cation channel subfamily M member 3, Long transient receptor potential channel 3, LTrpC3, Melastatin-2, MLSN2
  • Anti-TRPM8 (extracellular) Antibody
    KO Validate'
    Anti-TRPM8 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to TRPM8 Channel
    • Product Code: ACC-049
    • Short Description: A Rabbit Polyclonal Antibody to TRPM8 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Neutralization, Immunohistochemistry
    • Target: TRPP8, LTrpC6, Transient receptor potential cation channel subfamily M member 8
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