Inflammation

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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

2041 Results
  • Anti-TRPV2 (VRL1) (extracellular)-APC Antibody
    Anti-TRPV2 (VRL1) (extracellular)-APC Antibody
    A Rabbit Polyclonal Antibody to TRPV2 (VRL1) (extracellular) conjugated to the fluorescent dye Allophycocyanin (APC)
    • Product Code: ACC-039-APC
    • Short Description: A Rabbit Polyclonal Antibody to TRPV2 (VRL1) (extracellular) conjugated to the fluorescent dye Allophycocyanin (APC)
    • Host: Rabbit
    • Label: APC
    • Reactivity: Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Transient receptor potential cation channel subfamily V member 2, Vanilloid receptor-like protein 1, VRL-1, Osm-9-like TRP channel 2, OTRPC2, Stretch-activated channel 2B, SAC2B
  • Anti-TRPV2 (VRL1) (extracellular) Antibody
    Anti-TRPV2 (VRL1) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to TRPV2 (VRL1) Channel
    • Product Code: ACC-039
    • Short Description: A Rabbit Polyclonal Antibody to TRPV2 (VRL1) Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry
    • Target: Transient receptor potential cation channel subfamily V member 2, Vanilloid receptor-like protein 1, VRL-1, Osm-9-like TRP channel 2, OTRPC2, Stretch-activated channel 2B, SAC2B
  • Guinea Pig Anti-TRPA1 (extracellular) Antibody
    Guinea Pig Anti-TRPA1 (extracellular) Antibody
    A Guinea Pig Polyclonal Antibody to TRPA1 (extracellular)
    • Product Code: ACC-037-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to TRPA1 (extracellular)
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: Transient receptor potential cation channel subfamily A member 1, ANKTM1, Ankyrin-like with transmembrane domains protein 1, Transformation-sensitive protein p120, Wasabi Receptor
  • Anti-TRPA1 (extracellular)-FITC Antibody
    Anti-TRPA1 (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to TRPA1 (extracellular) conjugated to the fluorescent dye FITC
    • Product Code: ACC-037-F
    • Short Description: A Rabbit Polyclonal Antibody to TRPA1 (extracellular) conjugated to the fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Flow cytometry
    • Target: Transient receptor potential cation channel subfamily A member 1, ANKTM1, Ankyrin-like with transmembrane domains protein 1, Transformation-sensitive protein p120, Wasabi Receptor
  • Anti-TRPA1 (extracellular) Antibody
    KO Validate'
    Anti-TRPA1 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to TRPA1 Channel
    • Product Code: ACC-037
    • Short Description: A Rabbit Polyclonal Antibody to TRPA1 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry
    • Target: Transient receptor potential cation channel subfamily A member 1, ANKTM1, Ankyrin-like with transmembrane domains protein 1, Transformation-sensitive protein p120
  • Anti-TRPV6 Antibody
    Anti-TRPV6 Antibody
    A Rabbit Polyclonal Antibody to TRPV6 Channel
    • Product Code: ACC-036
    • Short Description: A Rabbit Polyclonal Antibody to TRPV6 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry
    • Target: Epithelial calcium channel 2, ECaC2, Calcium transport protein 1, CaT1, CaT-like, CaT-L, Transient receptor potential cation channel subfamily V member 6
  • Anti-TRPV5 Antibody
    Anti-TRPV5 Antibody
    A Rabbit Polyclonal Antibody to TRPV5 Channel
    • Product Code: ACC-035
    • Short Description: A Rabbit Polyclonal Antibody to TRPV5 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Transient receptor potential cation channel subfamily V member 5, Calcium transport protein 2, CaT2, Epithelial calcium channel 1, ECaC1, OTRPC3
  • Guinea pig Anti-TRPV4 Antibody
    Guinea pig Anti-TRPV4 Antibody
    A Guinea Pig Polyclonal Antibody to TRPV4 Channel
    • Product Code: ACC-034-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to TRPV4 Channel
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Mouse
    • Applications: Western blot
    • Target: OTRPC4, VRL-2, VR-OAC, TRP12
  • Anti-TRPV4-ATTO Fluor-550 Antibody
    KO Validate'
    Anti-TRPV4-ATTO Fluor-550 Antibody
    A Rabbit Polyclonal Antibody to TRPV4 Channel Conjugated to the Fluorescent Dye ATTO-550
    • Product Code: ACC-034-AO
    • Short Description: A Rabbit Polyclonal Antibody to TRPV4 Channel Conjugated to the Fluorescent Dye ATTO-550
    • Host: Rabbit
    • Label: ATTO 550
    • Reactivity: Human, Rat, Mouse
    • Applications: Immunofluorescence, Immunohistochemistry
    • Target: OTRPC4, VRL-2, VR-OAC, TRP12
  • Anti-TRPV4 Antibody
    KO Validate'
    Anti-TRPV4 Antibody
    A Rabbit Polyclonal Antibody to TRPV4 Channel
    • Product Code: ACC-034
    • Short Description: A Rabbit Polyclonal Antibody to TRPV4 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Western blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry
    • Target: OTRPC4, VRL-2, VR-OAC, TRP12
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