Inflammation

Inflammation

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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.
  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

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2041 Results
  • Anti-B1 Bradykinin Receptor (BDKRB1) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to B1 Bradykinin Receptor
    • Product Code: ABR-013
    • Short Description: A Rabbit Polyclonal Antibody to B1 Bradykinin Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot
    • Target: BK-1 receptor, BKRB1, B1R, BKR1
  • Anti-B<sub>2</sub> Bradykinin Receptor (BDKRB2) Antibody
    A Rabbit Polyclonal Antibody to B2 Bradykinin Receptor
    • Product Code: ABR-012
    • Short Description: A Rabbit Polyclonal Antibody to B2 Bradykinin Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: B2R, BKR2, B2BKR, BKRB2, Kinin B2, BRADYB2
  • Anti-B<sub>1</sub> Bradykinin Receptor (BDKRB1) Antibody
    A Rabbit Polyclonal Antibody to B1 Bradykinin Receptor
    • Product Code: ABR-011
    • Short Description: A Rabbit Polyclonal Antibody to B1 Bradykinin Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: B1R, BKR1, B1BKR, BKRB1, Kinin B1, BRADYB1
  • Anti-Bombesin Receptor 1 (NMBR) Antibody
    A Rabbit Polyclonal Antibody to Bombesin Receptor 1/Neuromedin B Receptor
    • Product Code: ABR-004
    • Short Description: A Rabbit Polyclonal Antibody to Bombesin Receptor 1/Neuromedin B Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: BB1R, Neuromedin B receptor, Neuromedin-B-preferring bombesin receptor
  • Anti-Bombesin Receptor 3 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Bombesin Receptor 3
    • Product Code: ABR-003
    • Short Description: A Rabbit Polyclonal Antibody to Bombesin Receptor 3
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: BB3R, BRS3
  • Anti-Bombesin Receptor 2 (GRPR) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Bombesin Receptor 2/Gastrin-releasing Peptide Receptor
    • Product Code: ABR-002
    • Short Description: A Rabbit Polyclonal Antibody to Bombesin Receptor 2/Gastrin-releasing Peptide Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: BB2R, Gastrin-releasing peptide receptor, GRP-preferring bombesin receptor
  • Anti-Bestrophin-2 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Bestrophin-2 Channel
    • Product Code: ABC-002
    • Short Description: A Rabbit Polyclonal Antibody to Bestrophin-2 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: BEST2, Vitelliform macular dystrophy 2-like protein 1, VMD2L1
  • Anti-Bestrophin-1 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Bestrophin-1 Channel
    • Product Code: ABC-001
    • Short Description: A Rabbit Polyclonal Antibody to Bestrophin-1 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: BEST1, Vitelliform macular dystrophy protein 2, VMD2, BMD1
  • Anti-Adenylate Cyclase 3 (AC3) (extracellular)-ATTO Fluor-488 Antibody
    A Rabbit Polyclonal Antibody to Adenylate Cyclase Type III Conjugated to the Fluorescent Dye ATTO-488
    • Product Code: AAR-043-AG
    • Short Description: A Rabbit Polyclonal Antibody to Adenylate Cyclase Type III Conjugated to the Fluorescent Dye ATTO-488
    • Host: Rabbit
    • Label: ATTO 488
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Immunocytochemistry, Immunofluorescence, Flow cytometry
    • Target: Adenylate cyclase type 3, Adenylate cyclase type III, AC-III, ATP pyrophosphate-lyase 3, Adenylate cyclase olfactive type, Adenylyl cyclase 3, ADCY3
  • Anti-Adenylate Cyclase 3 (AC3) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Adenylate Cyclase Type III
    • Product Code: AAR-043
    • Short Description: A Rabbit Polyclonal Antibody to Adenylate Cyclase Type III
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Adenylate cyclase type 3, Adenylate cyclase type III, AC-III, ATP pyrophosphate-lyase 3, Adenylate cyclase olfactive type, Adenylyl cyclase 3, ADCY3
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