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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

2045 Results
  • Mouse Anti-Human Orai1 (extracellular)-Biotin Antibody
    Mouse Anti-Human Orai1 (extracellular)-Biotin Antibody
    A Mouse Monoclonal Antibody to Human Orai1 Channel Conjugated to Biotin
    • Product Code: ALM-025-B
    • Short Description: A Mouse Monoclonal Antibody to Human Orai1 Channel Conjugated to Biotin
    • Host: Mouse
    • Label: Biotin
    • Reactivity: Human
    • Applications: Western blot, Flow cytometry
    • Target: TMEM142A, CRACM1, Calcium release-activated calcium channel protein 1
  • Mouse Anti-Human Orai1 (extracellular) Antibody
    Mouse Anti-Human Orai1 (extracellular) Antibody
    A Mouse Monoclonal Antibody to Human Orai1 Channel
    • Product Code: ALM-025
    • Short Description: A Mouse Monoclonal Antibody to Human Orai1 Channel
    • Host: Mouse
    • Label: Unconjugated
    • Reactivity: Human
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence
    • Target: TMEM142A, CRACM1, Calcium release-activated calcium channel protein 1
  • Mouse NGF/proNGF Neutralizing Antibody
    Mouse NGF/proNGF Neutralizing Antibody
    A Mouse Monoclonal Antibody to NGF and proNGF Designed to Neutralize their Biological Functions
    • Product Code: ALM-006
    • Short Description: A Mouse Monoclonal Antibody to NGF and proNGF Designed to Neutralize their Biological Functions
    • Host: Mouse
    • Label: Unconjugated
    • Reactivity: Human, Mouse
    • Applications: Indirect ELISA, Neutralization
    • Target: Nerve growth factor
  • Anti-KCC2 (SLC12A5) Antibody
    Anti-KCC2 (SLC12A5) Antibody
    A Rabbit Polyclonal Antibody to KCC2 Transporter
    • Product Code: AKT-005
    • Short Description: A Rabbit Polyclonal Antibody to KCC2 Transporter
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: K+-Cl- cotransporter 2, Electroneutral potassium-chloride cotransporter 2, Neuronal K-Cl cotransporter, Solute carrier family 12 member 5
  • Anti-KISS1 Receptor (GPR54) (extracellular)-FITC Antibody
    Anti-KISS1 Receptor (GPR54) (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to KISS1 Receptor (GPR54) (extracellular) conjugated to the fluorescent dye FITC
    • Product Code: AKR-001-F
    • Short Description: A Rabbit Polyclonal Antibody to KISS1 Receptor (GPR54) (extracellular) conjugated to the fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Kisspeptin receptor, KISS1R, Metastin receptor, AXOR12, G-Protein Coupled Receptor 54
  • Anti-KISS1 Receptor (GPR54) (extracellular) Antibody
    Anti-KISS1 Receptor (GPR54) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Kisspeptin Receptor
    • Product Code: AKR-001
    • Short Description: A Rabbit Polyclonal Antibody to Kisspeptin Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Kisspeptin receptor, KISS1R, Metastin receptor, AXOR12
  • Anti-Ferroportin (SLC40A1) Antibody
    Anti-Ferroportin (SLC40A1) Antibody
    A Rabbit Polyclonal Antibody to Ferroportin (SLC40A1)
    • Product Code: AIT-001
    • Short Description: A Rabbit Polyclonal Antibody to Ferroportin (SLC40A1)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Ferroportin-1, FPN1, Iron-regulated transporter 1, IREG1, Metal transporter protein, MTP1, SLC11A3, HFE4
  • Anti-CRMP2 Antibody
    Anti-CRMP2 Antibody
    A Rabbit Polyclonal Antibody to CRMP-2
    • Product Code: AIP-029
    • Short Description: A Rabbit Polyclonal Antibody to CRMP-2
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: CRMP-2, Collapsin response mediator protein 2, Dihydropyrimidinase-related protein 2, DHPRP2, DRP-2, Dpysl2, Turned on after division 64 kDa protein, TOAD-64, Ulip2, Unc-33-like phosphoprotein 2
  • Anti-CRMP1 Antibody
    Anti-CRMP1 Antibody
    A Rabbit Polyclonal Antibody to CRMP-1
    • Product Code: AIP-028
    • Short Description: A Rabbit Polyclonal Antibody to CRMP-1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot
    • Target: CRMP-1, Collapsin response mediator protein 1, Dihydropyrimidinase-related protein 1, DRP-1, Dpysl1, Dihydropyrimidinase-like 1, Ulip3, Unc-33-like phosphoprotein 3
  • Anti-Neurofascin Antibody
    Anti-Neurofascin Antibody
    A Rabbit Polyclonal Antibody to Neurofascin
    • Product Code: AIP-025
    • Short Description: A Rabbit Polyclonal Antibody to Neurofascin
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: NFASC, NRCAML, NF
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