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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

2041 Results
  • Anti-Presenilin-1-ATTO Fluor-488 Antibody
    Anti-Presenilin-1-ATTO Fluor-488 Antibody
    A Rabbit Polyclonal Antibody to Presenilin-1 Conjugated to the Fluorescent Dye ATTO-488
    • Product Code: AIP-011-AG
    • Short Description: A Rabbit Polyclonal Antibody to Presenilin-1 Conjugated to the Fluorescent Dye ATTO-488
    • Host: Rabbit
    • Label: ATTO 488
    • Reactivity: Human, Rat, Mouse
    • Applications: Immunofluorescence, Immunohistochemistry
    • Target: PSEN1, PS-1, Psnl1
  • Anti-Presenilin-1 Antibody
    Anti-Presenilin-1 Antibody
    A Rabbit Polyclonal Antibody to Presenilin-1
    • Product Code: AIP-011
    • Short Description: A Rabbit Polyclonal Antibody to Presenilin-1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: PSEN1, PS-1, Psnl1
  • Anti-Sigma-1 Receptor Antibody
    KO Validate'
    Anti-Sigma-1 Receptor Antibody
    A Rabbit Polyclonal Antibody to Sigma-1 Receptor
    • Product Code: AIP-006
    • Short Description: A Rabbit Polyclonal Antibody to Sigma-1 Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: SIGMAR1, OPRS1, Sigma non-opioid intracellular receptor 1, SIG-1R, Aging-associated gene 8 protein, SR31747-binding protein, SRBP
  • Anti-GPR109A/HCAR2 (extracellular)-FITC Antibody
    Anti-GPR109A/HCAR2 (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to GPR109A/HCAR2 (extracellular) conjugated to the fluorescent dye FITC
    • Product Code: AHR-012-F
    • Short Description: A Rabbit Polyclonal Antibody to GPR109A/HCAR2 (extracellular) conjugated to the fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Hydroxycarboxylic Acid Receptor 2, Niacin Receptor 1, G-Protein Coupled Receptor HM74A, HM74A, Nicotinic acid receptor, PUMAG
  • Anti-GPR109A/HCAR2 (extracellular) Antibody
    Anti-GPR109A/HCAR2 (extracellular) Antibody
    A Rabbit Polyclonal antibody to GPR109A/HCAR2 (extracellular)
    • Product Code: AHR-012
    • Short Description: A Rabbit Polyclonal antibody to GPR109A/HCAR2 (extracellular)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: Hydroxycarboxylic Acid Receptor 2, Niacin Receptor 1, G-Protein Coupled Receptor HM74A, HM74A, Nicotinic acid receptor, PUMAG
  • Anti-Histamine H1 Receptor/HRH1 (extracellular)-FITC Antibody
    Anti-Histamine H1 Receptor/HRH1 (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to H1R (extracellular) conjugated to the fluorescent dye FITC
    • Product Code: AHR-006-F
    • Short Description: A Rabbit Polyclonal Antibody to H1R (extracellular) conjugated to the fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Flow cytometry
    • Target: Histamine Receptor H1, H1R
  • Anti-Histamine H<sub>1 </sub> Receptor/HRH1 (extracellular) Antibody
    Anti-Histamine H1 Receptor/HRH1 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Histamine H1 Receptor
    • Product Code: AHR-006
    • Short Description: A Rabbit Polyclonal Antibody to Histamine H1 Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Histamine Receptor H1, H1R
  • Anti-Human Histamine H<sub>4</sub> Receptor (HRH4) (extracellular) Antibody
    Anti-Human Histamine H4 Receptor (HRH4) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Human Histamine H4 Receptor
    • Product Code: AHR-004
    • Short Description: A Rabbit Polyclonal Antibody to Human Histamine H4 Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot
    • Target: H4R, HH4R, G-protein coupled receptor 105, GPCR105, GPRv53, AXOR35
  • Anti-Histamine H<sub>2</sub> Receptor (HRH2) (extracellular) Antibody
    Anti-Histamine H2 Receptor (HRH2) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Histamine H2 Receptor
    • Product Code: AHR-002
    • Short Description: A Rabbit Polyclonal Antibody to Histamine H2 Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: H2R, HH2R, Gastric receptor I, Gastric receptor 1
  • Anti-HVCN1 (extracellular) Antibody
    Anti-HVCN1 (extracellular) Antibody
    A rabbit polyclonal antibody to HVCN1 (extracellular)
    • Product Code: AHC-002
    • Short Description: A rabbit polyclonal antibody to HVCN1 (extracellular)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot
    • Target: HVCN1
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