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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

2041 Results
  • Guinea pig Anti-Vesicular GABA Transporter (VGAT) Antibody
    Guinea pig Anti-Vesicular GABA Transporter (VGAT) Antibody
    A Guinea Pig Polyclonal Antibody to Vesicular GABA Transporter
    • Product Code: AGT-005-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to Vesicular GABA Transporter
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: Vesicular inhibitory amino acid transporter, VIAAT, GABA and glycine transporter, rGVAT, SLC32A1
  • Anti-Vesicular GABA Transporter (VGAT)-ATTO Fluor-594 Antibody
    Anti-Vesicular GABA Transporter (VGAT)-ATTO Fluor-594 Antibody
    A Rabbit Polyclonal Antibody to Vesicular GABA Transporter Conjugated to the Fluorescent Dye ATTO-594
    • Product Code: AGT-005-AR
    • Short Description: A Rabbit Polyclonal Antibody to Vesicular GABA Transporter Conjugated to the Fluorescent Dye ATTO-594
    • Host: Rabbit
    • Label: ATTO 594
    • Reactivity: Human, Rat, Mouse
    • Applications: Immunofluorescence, Immunohistochemistry
    • Target: Vesicular inhibitory amino acid transporter, VIAAT, GABA and glycine transporter, SLC32A1
  • Anti-Vesicular GABA Transporter (VGAT) Antibody
    Anti-Vesicular GABA Transporter (VGAT) Antibody
    A Rabbit Polyclonal Antibody to Vesicular GABA Transporter
    • Product Code: AGT-005
    • Short Description: A Rabbit Polyclonal Antibody to Vesicular GABA Transporter
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Vesicular inhibitory amino acid transporter, VIAAT, GABA and glycine transporter, SLC32A1
  • Anti-GABA Transporter 3 (GAT-3) Antibody
    Anti-GABA Transporter 3 (GAT-3) Antibody
    A Rabbit Polyclonal Antibody to GABA Transporter 3 (GAT-3)
    • Product Code: AGT-003
    • Short Description: A Rabbit Polyclonal Antibody to GABA Transporter 3 (GAT-3)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot
    • Target: SLC6A11, GABT3, Na+/Cl- -dependent GABA transporter 3
  • Guinea Pig Anti-GABA Transporter 1 (GAT-1) (extracellular) Antibody
    Guinea Pig Anti-GABA Transporter 1 (GAT-1) (extracellular) Antibody
    A Guinea Pig Polyclonal Antibody to GABA Transporter 1 (GAT-1) (extracellular)
    • Product Code: AGT-001-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to GABA Transporter 1 (GAT-1) (extracellular)
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: GABATR, GABT1, GABATHG, Na+/Cl-- dependent GABA transporter 1, Solute Carrier Family 6 Member 1, SLC6A1
  • Anti-GABA Transporter 1 (GAT-1) (extracellular) Antibody
    Anti-GABA Transporter 1 (GAT-1) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to GABA Transporter 1 (GAT-1)
    • Product Code: AGT-001
    • Short Description: A Rabbit Polyclonal Antibody to GABA Transporter 1 (GAT-1)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunofluorescence, Immunohistochemistry
    • Target: GABATR, GABT1, GABATHG, SLC6A1, Na+/Cl-- dependent GABA transporter 1
  • Anti-GPR173 Antibody
    Anti-GPR173 Antibody
    A Rabbit Polyclonal Antibody to GPR173
    • Product Code: AGR-093
    • Short Description: A Rabbit Polyclonal Antibody to GPR173
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: G-Protein Coupled Receptor 173, Super Conserved Receptor Expressed In Brain 3, SREB3
  • Anti-GPRC5A Antibody
    Anti-GPRC5A Antibody
    A Rabbit Polyclonal Antibody to GPRC5A
    • Product Code: AGR-071
    • Short Description: A Rabbit Polyclonal Antibody to GPRC5A
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot
    • Target: Retinoic Acid-Induced Gene 1 Protein; RAIG1; Retinoic Acid Induced 3; RAI3; G Protein-Coupled Receptor Class C Group 5 Member A
  • Anti-GPR18 (extracellular) Antibody
    Anti-GPR18 (extracellular) Antibody
    A Rabbit Polyclonal antibody to GPR18 (extracellular)
    • Product Code: AGR-068
    • Short Description: A Rabbit Polyclonal antibody to GPR18 (extracellular)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: G-Protein Coupled Receptor 18, N-Arachidonyl Glycine Receptor, NAGly Receptor, GPCRW
  • Anti-GPR31 (extracellular) Antibody
    Anti-GPR31 (extracellular) Antibody
    A Rabbit Polyclonal antibody to GPR31 (extracellular)
    • Product Code: AGR-064
    • Short Description: A Rabbit Polyclonal antibody to GPR31 (extracellular)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: G Protein-Coupled Receptor 31, 12-(S)-HETE Acid Receptor, 12-HETER, GPR31/12-HETER
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