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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

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2041 Results
  • Anti-Ghrelin Receptor (GHSR) (extracellular) Antibody
    KO Validate'
    Anti-Ghrelin Receptor (GHSR) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Ghrelin Receptor (GHSR)
    • Product Code: AGR-031
    • Short Description: A Rabbit Polyclonal Antibody to Ghrelin Receptor (GHSR)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Growth hormone secretagogue receptor type 1, GHS-R, GH-releasing peptide receptor, GHRP
  • Anti-GnRH Receptor (extracellular) Antibody
    Anti-GnRH Receptor (extracellular) Antibody
    A Rabbit Polyclonal Antibody to GnRH Receptor
    • Product Code: AGR-030
    • Short Description: A Rabbit Polyclonal Antibody to GnRH Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Gonadotropin-releasing hormone receptor, GnRHR, Leutinizing hormone releasing hormone receptor, LHRHR, Luliberin receptor
  • Anti-Secretin Receptor (extracellular) Antibody
    Anti-Secretin Receptor (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Secretin Receptor
    • Product Code: AGR-026
    • Short Description: A Rabbit Polyclonal Antibody to Secretin Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: SCTR
  • Anti-GIPR (extracellular) Antibody
    Anti-GIPR (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Gastric Inhibitory Polypeptide Receptor
    • Product Code: AGR-025
    • Short Description: A Rabbit Polyclonal Antibody to Gastric Inhibitory Polypeptide Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Gastric inhibitory polypeptide receptor, GIP receptor, Glucose-dependent insulinotropic polypeptide receptor
  • Anti-Glucagon Receptor (extracellular) Antibody
    Anti-Glucagon Receptor (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Glucagon Receptor
    • Product Code: AGR-024
    • Short Description: A Rabbit Polyclonal Antibody to Glucagon Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: GCGR, GLR
  • Anti-GLP1R (extracellular)-PE Antibody
    Anti-GLP1R (extracellular)-PE Antibody
    A Rabbit Polyclonal Antibody to GLP1R conjugated to R-Phycoerythrin (R-PE) fluorophore
    • Product Code: AGR-021-PE
    • Short Description: A Rabbit Polyclonal Antibody to GLP1R conjugated to R-Phycoerythrin (R-PE) fluorophore
    • Host: Rabbit
    • Label: R-PE
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Glucagon-like peptide 1 receptor, GLP-1 receptor
  • Anti-GLP1R (extracellular)-FITC Antibody
    Anti-GLP1R (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to Glucagon-like Peptide 1 Receptor Conjugated to the Fluorescent Dye FITC
    • Product Code: AGR-021-F
    • Short Description: A Rabbit Polyclonal Antibody to Glucagon-like Peptide 1 Receptor Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Glucagon-like peptide 1 receptor, GLP-1 receptor
  • Anti-GLP1R (extracellular) Antibody
    KO Validate'
    Anti-GLP1R (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Glucagon-like Peptide 1 Receptor
    • Product Code: AGR-021
    • Short Description: A Rabbit Polyclonal Antibody to Glucagon-like Peptide 1 Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Glucagon-like peptide 1 receptor, GLP-1 receptor
  • Anti-Glycine Receptor α3 Antibody
    Anti-Glycine Receptor α3 Antibody
    A Rabbit Polyclonal Antibody to Glycine Receptor α3
    • Product Code: AGR-003
    • Short Description: A Rabbit Polyclonal Antibody to Glycine Receptor α3
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: Glycine receptor subunit alpha-3, GLRA3, GlyRα3
  • Anti-GRIK4 (KA1) (extracellular) Antibody
    KO Validate'
    Anti-GRIK4 (KA1) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Kainate Receptor GluK4
    • Product Code: AGC-041
    • Short Description: A Rabbit Polyclonal Antibody to Kainate Receptor GluK4
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Glutamate receptor ionotropic kainate 4, GluK4, Glutamate receptor KA-1
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