Inflammation

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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

2045 Results
  • Anti-GluR4 (GluA4) (extracellular) Antibody
    Anti-GluR4 (GluA4) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to AMPA-Selective Glutamate Receptor 4 (GluR4)
    • Product Code: AGC-019
    • Short Description: A Rabbit Polyclonal Antibody to AMPA-Selective Glutamate Receptor 4 (GluR4)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: AMPA Receptor 4, Glutamate receptor 4, Ionotropic glutamate receptor 4, AMPA-selective glutamate receptor 4, GRIA4, GluR-D
  • Anti-NMDAR2C (GRIN2C) (extracellular) Antibody
    Anti-NMDAR2C (GRIN2C) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to NMDA Receptor 2C (GRIN2C)
    • Product Code: AGC-018
    • Short Description: A Rabbit Polyclonal Antibody to NMDA Receptor 2C (GRIN2C)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: NMDA receptor 2C, NR2C, GluN2C, Ionotropic glutamate receptor subunit ε3, N-methyl-D-aspartate receptor subunit 2C
  • Anti-mGluR7 (extracellular) Antibody
    Anti-mGluR7 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Metabotropic Glutamate Receptor 7
    • Product Code: AGC-017
    • Short Description: A Rabbit Polyclonal Antibody to Metabotropic Glutamate Receptor 7
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Metabotropic glutamate receptor 7, GRM7, mGlu7, Gprc1g
  • Anti-mGluR4 (extracellular) Antibody
    Anti-mGluR4 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Metabotropic Glutamate Receptor 4
    • Product Code: AGC-014
    • Short Description: A Rabbit Polyclonal Antibody to Metabotropic Glutamate Receptor 4
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence
    • Target: Metabotropic glutamate receptor 4, GRM4, mGlu4, GPRC1D
  • Anti-mGluR3 (extracellular) Antibody
    KO Validate'
    Anti-mGluR3 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Metabotropic Glutamate Receptor 3
    • Product Code: AGC-012
    • Short Description: A Rabbit Polyclonal Antibody to Metabotropic Glutamate Receptor 3
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Metabotropic glutamate receptor 3, GRM3, mGlu3, GPRC1C
  • Anti-mGluR2 (extracellular) Antibody
    Anti-mGluR2 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Metabotropic Glutamate Receptor 2
    • Product Code: AGC-011
    • Short Description: A Rabbit Polyclonal Antibody to Metabotropic Glutamate Receptor 2
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Metabotropic glutamate receptor 2, GRM2, mGlu2, GPRC1B
  • Guinea pig Anti-GluR3 (GluA3) (extracellular) Antibody
    Guinea pig Anti-GluR3 (GluA3) (extracellular) Antibody
    A Guinea Pig Polyclonal Antibody to AMPA-Selective Glutamate Receptor 3 (GluR3)
    • Product Code: AGC-010-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to AMPA-Selective Glutamate Receptor 3 (GluR3)
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: AMPA receptor 3, Glutamate receptor 3, Ionotropic glutamate receptor 3, AMPA-selective glutamate receptor 3, GRIA3, GluR-C, GluR-K3
  • Anti-GluR3 (GluA3) (extracellular) Antibody
    Anti-GluR3 (GluA3) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to AMPA-Selective Glutamate Receptor 3 (GluR3)
    • Product Code: AGC-010
    • Short Description: A Rabbit Polyclonal Antibody to AMPA-Selective Glutamate Receptor 3 (GluR3)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry
    • Target: AMPA receptor 3, Glutamate receptor 3, Ionotropic glutamate receptor 3, AMPA-selective glutamate receptor 3, GRIA3, GluR-C, GluR-K3
  • Anti-GRIK2 (GluK2) Antibody
    Anti-GRIK2 (GluK2) Antibody
    A Rabbit Polyclonal Antibody to Kainate Receptor GluK2
    • Product Code: AGC-009
    • Short Description: A Rabbit Polyclonal Antibody to Kainate Receptor GluK2
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence
    • Target: Glutamate receptor ionotropic kainate 2, Glutamate receptor 6, GluR6, GLUK6
  • Anti-GRIK1 (GluK1) (extracellular) Antibody
    Anti-GRIK1 (GluK1) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Kainate Receptor GluK1
    • Product Code: AGC-008
    • Short Description: A Rabbit Polyclonal Antibody to Kainate Receptor GluK1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Mouse
    • Applications: Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Glutamate receptor ionotropic kainate 1, Glutamate receptor 5, GluR5
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