Inflammation

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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

2041 Results
  • Anti-NMDAR2A (GluN2A) (extracellular)-ATTO Fluor-488 Antibody
    Anti-NMDAR2A (GluN2A) (extracellular)-ATTO Fluor-488 Antibody
    A Rabbit Polyclonal Antibody to NMDA Receptor 2A (GluN2A) Conjugated to the Fluorescent Dye ATTO-488
    • Product Code: AGC-002-AG
    • Short Description: A Rabbit Polyclonal Antibody to NMDA Receptor 2A (GluN2A) Conjugated to the Fluorescent Dye ATTO-488
    • Host: Rabbit
    • Label: ATTO 488
    • Reactivity: Human, Rat, Mouse
    • Applications: Immunofluorescence, Immunohistochemistry
    • Target: NMDA receptor 2A, NR2A, GRIN2A, Ionotropic glutamate receptor subunit ε1, N-methyl-D-aspartate receptor subunit 2A
  • Anti-NMDAR2A (GluN2A) (extracellular) Antibody
    Anti-NMDAR2A (GluN2A) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to NMDA Receptor 2A (GluN2A)
    • Product Code: AGC-002
    • Short Description: A Rabbit Polyclonal Antibody to NMDA Receptor 2A (GluN2A)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry
    • Target: NMDA receptor 2A, NR2A, GRIN2A, Ionotropic glutamate receptor subunit ε1, N-methyl-D-aspartate receptor subunit 2A
  • Guinea pig Anti-NMDAR1 (GluN1) (extracellular) Antibody
    Guinea pig Anti-NMDAR1 (GluN1) (extracellular) Antibody
    A Guinea Pig Polyclonal Antibody to NMDA Receptor 1 (GluN1)
    • Product Code: AGC-001-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to NMDA Receptor 1 (GluN1)
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: NMDA receptor 1, Ionotropic glutamate receptor subunit ζ 1, N-methyl-D-aspartate receptor subunit 1, GRIN1
  • Anti-NMDAR1 (GluN1) (extracellular) Antibody
    Anti-NMDAR1 (GluN1) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to NMDA Receptor 1 (GluN1)
    • Product Code: AGC-001
    • Short Description: A Rabbit Polyclonal Antibody to NMDA Receptor 1 (GluN1)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: NMDA receptor 1, Ionotropic glutamate receptor subunit ζ 1, N-methyl-D-aspartate receptor subunit 1, GRIN1
  • Anti-GABA(B) R2 Antibody
    Anti-GABA(B) R2 Antibody
    A Rabbit Polyclonal Antibody to GABA(B) Receptor 2
    • Product Code: AGB-002
    • Short Description: A Rabbit Polyclonal Antibody to GABA(B) Receptor 2
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: γ-Aminobutyric acid type B receptor subunit 2, GABA B receptor 2, GABABR2, Gb2, GABBR2, GPR51, GPRC3B, HG20
  • Anti-GABA(B) Receptor 1 (extracellular)-FITC Antibody
    Anti-GABA(B) Receptor 1 (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to GABBR1 (extracellular) conjugated to the fluorescent dye FITC
    • Product Code: AGB-001-F
    • Short Description: A Rabbit Polyclonal Antibody to GABBR1 (extracellular) conjugated to the fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: γ-Aminobutyric acid type B receptor subunit 1, GABA B receptor 1, GABABR1, Gb1, GABBR1, GPRC3A
  • Anti-GABA(B) R1 (extracellular) Antibody
    Anti-GABA(B) R1 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to GABA(B) Receptor 1
    • Product Code: AGB-001
    • Short Description: A Rabbit Polyclonal Antibody to GABA(B) Receptor 1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunofluorescence, Immunohistochemistry
    • Target: γ-Aminobutyric acid type B receptor subunit 1, GABA B receptor 1, GABABR1, Gb1, GABBR1, GPRC3A
  • Anti-GABA(A) δ Receptor (extracellular) Antibody
    KO Validate'
    Anti-GABA(A) δ Receptor (extracellular) Antibody
    A Rabbit Polyclonal Antibody to GABA(A) δ Receptor
    • Product Code: AGA-014
    • Short Description: A Rabbit Polyclonal Antibody to GABA(A) δ Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunofluorescence, Immunohistochemistry
    • Target: GABRD, Gamma-aminobutyric acid receptor subunit delta, GABA(A) receptor subunit delta
  • Anti-GABA(A) β1 Receptor Antibody
    Anti-GABA(A) β1 Receptor Antibody
    A Rabbit Polyclonal Antibody to GABA(A) β1 Receptor
    • Product Code: AGA-010
    • Short Description: A Rabbit Polyclonal Antibody to GABA(A) β1 Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot
    • Target: γ-Aminobutyric acid receptor subunit β1, GABAA receptor beta-1, GABRB1, GARB1
  • Anti-GABA(A) α4 Receptor (extracellular) Antibody
    Anti-GABA(A) α4 Receptor (extracellular) Antibody
    A Rabbit Polyclonal Antibody to GABA(A) α4 Receptor
    • Product Code: AGA-008
    • Short Description: A Rabbit Polyclonal Antibody to GABA(A) α4 Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: γ-Aminobutyric acid receptor subunit α4, GABRA4
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