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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

2041 Results
  • Anti-GPR43/FFAR2 Antibody
    Anti-GPR43/FFAR2 Antibody
    A Rabbit Polyclonal Antibody to Free Fatty Acid Receptor 2 (GPR43)
    • Product Code: AFR-032
    • Short Description: A Rabbit Polyclonal Antibody to Free Fatty Acid Receptor 2 (GPR43)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: G-protein coupled receptor 43, Free fatty acid receptor 2
  • Anti-Frizzled-3 (FZD3) Antibody
    Anti-Frizzled-3 (FZD3) Antibody
    A Rabbit Polyclonal antibody to Frizzled-3 receptor.
    • Product Code: AFR-023
    • Short Description: A Rabbit Polyclonal antibody to Frizzled-3 receptor.
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: Frizzled Class Receptor 3, Fz-3
  • Anti-Frizzled-2 (FZD2) (extracellular) Antibody
    Anti-Frizzled-2 (FZD2) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Frizzled-2
    • Product Code: AFR-022
    • Short Description: A Rabbit Polyclonal Antibody to Frizzled-2
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot
    • Target: Frizzled class receptor 2, Fz-2
  • Anti-Human GPR43/FFAR2 (extracellular) Antibody
    Anti-Human GPR43/FFAR2 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Human Free Fatty Acid Receptor 2 (GPR43)
    • Product Code: AFR-015
    • Short Description: A Rabbit Polyclonal Antibody to Human Free Fatty Acid Receptor 2 (GPR43)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot
    • Target: G-protein coupled receptor 43, Free fatty acid receptor 2
  • Anti-GPR120/FFAR4 (extracellular)-FITC Antibody
    Anti-GPR120/FFAR4 (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to GPR120/FFAR4 Conjugated to the Fluorescent Dye FITC
    • Product Code: AFR-014-F
    • Short Description: A Rabbit Polyclonal Antibody to GPR120/FFAR4 Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Flow cytometry
    • Target: G-protein coupled receptor 120, Free fatty acid receptor 4, FFA4 Receptor, G-protein coupled receptor GT01, Omega-3 fatty acid receptor 1, O3far1
  • Anti-GPR120/FFAR4 (extracellular) Antibody
    Anti-GPR120/FFAR4 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Free Fatty Acid Receptor 4 (GPR120)
    • Product Code: AFR-014
    • Short Description: A Rabbit Polyclonal Antibody to Free Fatty Acid Receptor 4 (GPR120)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: G-protein coupled receptor 120, Free fatty acid receptor 4, FFA4 Receptor, G-protein coupled receptor GT01, Omega-3 fatty acid receptor 1, O3far1
  • Anti-GPR40/FFAR1 (extracellular) Antibody
    Anti-GPR40/FFAR1 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Free Fatty Acid Receptor 1 (GPR40)
    • Product Code: AFR-011
    • Short Description: A Rabbit Polyclonal Antibody to Free Fatty Acid Receptor 1 (GPR40)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunofluorescence, Immunohistochemistry
    • Target: G-protein coupled receptor 40, Free fatty acid receptor 1
  • Anti-FSHR (extracellular) Antibody
    Anti-FSHR (extracellular) Antibody
    A Rabbit Polyclonal Antibody to FSH Receptor
    • Product Code: AFR-010
    • Short Description: A Rabbit Polyclonal Antibody to FSH Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Mouse
    • Applications: Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: FSH receptor, Follicle-stimulating hormone receptor, Follitropin receptor, FSHRO
  • Anti-Human FPR2/ALX (extracellular) Antibody
    Anti-Human FPR2/ALX (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Human FPR2/ALX
    • Product Code: AFR-002
    • Short Description: A Rabbit Polyclonal Antibody to Human FPR2/ALX
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunofluorescence, Immunohistochemistry
    • Target: N-formyl peptide receptor 2, FMLP-related receptor I, FMLPX, Formyl peptide receptor-like 1, FPRL1, ALXR, Lipoxin A4 receptor, LXA4 receptor, LXA4R, RFP
  • Anti-Human FPR1 (extracellular)-ATTO Fluor-488 Antibody
    Anti-Human FPR1 (extracellular)-ATTO Fluor-488 Antibody
    A Rabbit Polyclonal Antibody to FPR1 Conjugated to the Fluorescent Dye ATTO-488
    • Product Code: AFR-001-AG
    • Short Description: A Rabbit Polyclonal Antibody to FPR1 Conjugated to the Fluorescent Dye ATTO-488
    • Host: Rabbit
    • Label: ATTO 488
    • Reactivity: Human
    • Applications: Live cell imaging, Flow cytometry
    • Target: N-formyl peptide receptor 1, fMLP receptor
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