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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

2041 Results
  • Anti-GPER1/GPR30 (extracellular) Antibody
    Anti-GPER1/GPR30 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to GPER1/GPR30
    • Product Code: AER-049
    • Short Description: A Rabbit Polyclonal Antibody to GPER1/GPR30
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Western blot
    • Target: G protein-coupled estrogen receptor 1, G protein-coupled receptor 30, Membrane estrogen receptor, Chemoattractant receptor-like 2, GPER
  • Anti-EphA4 (extracellular) Antibody
    Anti-EphA4 (extracellular) Antibody
    A Rabbit Polyclonal antibody to EphA4 (extracellular).
    • Product Code: AER-044
    • Short Description: A Rabbit Polyclonal antibody to EphA4 (extracellular).
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot
    • Target: Ephrin Type-A Receptor 4, EPH-Like Kinase 8, EPHA4
  • Anti-Ephrin-B2 (extracellular) Antibody
    Anti-Ephrin-B2 (extracellular) Antibody
    A Rabbit Polyclonal antibody to Ephrin-B2 (extracellular)
    • Product Code: AER-042
    • Short Description: A Rabbit Polyclonal antibody to Ephrin-B2 (extracellular)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: EFNB2, EPH-Related Receptor Tyrosine Kinase Ligand 5, LERK5, HTK ligand, HTKL
  • Anti-Ephrin-B1 (extracellular) Antibody
    Anti-Ephrin-B1 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Ephrin-B1
    • Product Code: AER-041
    • Short Description: A Rabbit Polyclonal Antibody to Ephrin-B1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot
    • Target: EPH-related receptor tyrosine kinase ligand 2, LERK2, EFNB1
  • Anti-Ephrin-A1 (extracellular) Antibody
    Anti-Ephrin-A1 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Ephrin-A1
    • Product Code: AER-031
    • Short Description: A Rabbit Polyclonal Antibody to Ephrin-A1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence
    • Target: EPH-related receptor tyrosine kinase ligand 1, LERK1, EFNA1
  • Anti-EphB4 (extracellular) Antibody
    Anti-EphB4 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to EphB4 Receptor
    • Product Code: AER-024
    • Short Description: A Rabbit Polyclonal Antibody to EphB4 Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Ephrin type-B receptor 4, Eph receptor B4, Hepatoma transmembrane kinase, HTK
  • Anti-EphB1 (extracellular) Antibody
    Anti-EphB1 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to EphB1 Receptor
    • Product Code: AER-021
    • Short Description: A Rabbit Polyclonal Antibody to EphB1 Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence
    • Target: Ephrin type-B receptor 1, Eph receptor B1, Tyrosine-protein kinase receptor EPH-2
  • Anti-EphA2 (extracellular) Antibody
    Anti-EphA2 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to EphA2 Receptor
    • Product Code: AER-019
    • Short Description: A Rabbit Polyclonal Antibody to EphA2 Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: Ephrin type A receptor 2, EPH Receptor A2, Epithelial Cell Kinase, Tyrosine-Protein Kinase Receptor ECK, ECK, EPHA2
  • Anti-EphA3 (extracellular) Antibody
    Anti-EphA3 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to EphA3 Receptor
    • Product Code: AER-013
    • Short Description: A Rabbit Polyclonal Antibody to EphA3 Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Ephrin type-A receptor 3, EPH-like kinase 4, EK4, Tyrosine-protein kinase TYRO4
  • Anti-EphA1 (extracellular)-FITC Antibody
    Anti-EphA1 (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to EphA1 (extracellular) conjugated to the fluorescent dye FITC
    • Product Code: AER-011-F
    • Short Description: A Rabbit Polyclonal Antibody to EphA1 (extracellular) conjugated to the fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Ephrin type-A receptor 1, Eph receptor A1, EPH tyrosine kinase 1, Tyrosine-protein kinase receptor EPH, EPHT1
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