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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

2041 Results
  • Anti-EphA1 (extracellular) Antibody
    Anti-EphA1 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to EphA1 Receptor
    • Product Code: AER-011
    • Short Description: A Rabbit Polyclonal Antibody to EphA1 Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Ephrin type-A receptor 1, Eph receptor A1, EPH tyrosine kinase 1, Tyrosine-protein kinase receptor EPH, EPHT1
  • Anti-Endothelin Receptor B (extracellular) Antibody
    Anti-Endothelin Receptor B (extracellular) Antibody
    A Rabbit Polyclonal antibody to Endothelin Receptor B (extracellular)
    • Product Code: AER-005
    • Short Description: A Rabbit Polyclonal antibody to Endothelin Receptor B (extracellular)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot
    • Target: EDNRB, ET-B, ETB, ETRB, Endothelin B receptor, ETBR
  • Anti-Endothelin Receptor A (extracellular) Antibody
    Anti-Endothelin Receptor A (extracellular) Antibody
    A Rabbit Ployclonal Antibody to Endothelin Receptor A
    • Product Code: AER-004
    • Short Description: A Rabbit Ployclonal Antibody to Endothelin Receptor A
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot
    • Target: EDNRA, ET-A, ET-AR, ETRA, ET1-Specific type endothelin receptor, Endothelin-1 receptor, Endothelin Receptor Type A
  • Guinea pig Anti-Endothelin Receptor B Antibody
    Guinea pig Anti-Endothelin Receptor B Antibody
    A Guinea Pig Polyclonal Antibody to Endothelin Receptor B
    • Product Code: AER-002-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to Endothelin Receptor B
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot
    • Target: EDNRB, ET-B, ETB, ETRB, Endothelin B receptor, Endothelin receptor non-selective type
  • Anti-Endothelin Receptor B Antibody
    Anti-Endothelin Receptor B Antibody
    A Rabbit Polyclonal Antibody to Endothelin Receptor B
    • Product Code: AER-002
    • Short Description: A Rabbit Polyclonal Antibody to Endothelin Receptor B
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: EDNRB, ET-B, ETB, ETRB, Endothelin B receptor, Endothelin receptor non-selective type
  • Anti-Endothelin Receptor A Antibody
    Anti-Endothelin Receptor A Antibody
    A Rabbit Polyclonal Antibody to Endothelin Receptor A
    • Product Code: AER-001
    • Short Description: A Rabbit Polyclonal Antibody to Endothelin Receptor A
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: EDNRA, ETA, ET-AR, ETRA, ET1-Specific type endothelin receptor, Endothelin-1 receptor
  • Anti-DARPP32 Antibody
    Anti-DARPP32 Antibody
    A Rabbit Polyclonal Antibody to DARPP32
    • Product Code: ADR-006
    • Short Description: A Rabbit Polyclonal Antibody to DARPP32
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: Dopamine and cAMP-regulated neuronal phosphoprotein, Protein phosphatase 1 regulatory subunit 1B, PPP1R1B
  • Anti-D<sub>5</sub> Dopamine Receptor (extracellular) Antibody
    Anti-D5 Dopamine Receptor (extracellular) Antibody
    A Rabbit Polyclonal Antibody to D5 Dopamine Receptor
    • Product Code: ADR-005
    • Short Description: A Rabbit Polyclonal Antibody to D5 Dopamine Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunofluorescence, Immunohistochemistry
    • Target: DRD5, D(1B) dopamine receptor
  • Anti-D<sub>4</sub> Dopamine Receptor Antibody
    Anti-D4 Dopamine Receptor Antibody
    A Rabbit Polyclonal Antibody to D4 Dopamine Receptor
    • Product Code: ADR-004
    • Short Description: A Rabbit Polyclonal Antibody to D4 Dopamine Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot
    • Target: DRD4, D4DR, D(2C) dopamine receptor
  • Anti-D<sub>3</sub> Dopamine Receptor (extracellular) Antibody
    KO Validate'
    Anti-D3 Dopamine Receptor (extracellular) Antibody
    A Rabbit Polyclonal Antibody to D3 Dopamine Receptor
    • Product Code: ADR-003
    • Short Description: A Rabbit Polyclonal Antibody to D3 Dopamine Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunofluorescence, Immunohistochemistry
    • Target: DRD3
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