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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

2041 Results
  • Anti-CXCR4 (extracellular)-FITC Antibody
    Anti-CXCR4 (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to CXCR4 (extracellular) conjugated to the fluorescent dye FITC
    • Product Code: ACR-014-F
    • Short Description: A Rabbit Polyclonal Antibody to CXCR4 (extracellular) conjugated to the fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: C-X-C chemokine receptor type 4, CD184, Fusin, LESTR, NPYRL, SDF-1 receptor, WHIMS
  • Anti-CXCR4 (extracellular)-ATTO Fluor-488 Antibody
    Anti-CXCR4 (extracellular)-ATTO Fluor-488 Antibody
    A Rabbit Polyclonal Antibody to CXCR4 Chemokine Receptor Conjugated to the Fluorescent Dye ATTO-488
    • Product Code: ACR-014-AG
    • Short Description: A Rabbit Polyclonal Antibody to CXCR4 Chemokine Receptor Conjugated to the Fluorescent Dye ATTO-488
    • Host: Rabbit
    • Label: ATTO 488
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: C-X-C chemokine receptor type 4, CD184, Fusin, LESTR, NPYRL, SDF-1 receptor, WHIMS
  • Anti-CXCR4 (extracellular) Antibody
    Anti-CXCR4 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to CXCR4 Chemokine Receptor
    • Product Code: ACR-014
    • Short Description: A Rabbit Polyclonal Antibody to CXCR4 Chemokine Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: C-X-C chemokine receptor type 4, CD184, Fusin, LESTR, NPYRL, SDF-1 receptor, WHIMS
  • Anti-Human CXCR2 (extracellular)-ATTO Fluor-488 Antibody
    Anti-Human CXCR2 (extracellular)-ATTO Fluor-488 Antibody
    A Rabbit Polyclonal Antibody to CXCR2 Chemokine Receptor Conjugated to the Fluorescent Dye ATTO-488
    • Product Code: ACR-012-AG
    • Short Description: A Rabbit Polyclonal Antibody to CXCR2 Chemokine Receptor Conjugated to the Fluorescent Dye ATTO-488
    • Host: Rabbit
    • Label: ATTO 488
    • Reactivity: Human
    • Applications: Live cell imaging, Flow cytometry
    • Target: C-X-C chemokine receptor type 2, Interleukin-8 receptor B, IL-8R B, IL8RB, IL-8 receptor type 2, CD182
  • Anti-Human CXCR2 (extracellular) Antibody
    Anti-Human CXCR2 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to CXCR2 Chemokine Receptor
    • Product Code: ACR-012
    • Short Description: A Rabbit Polyclonal Antibody to CXCR2 Chemokine Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot
    • Target: C-X-C chemokine receptor type 2, Interleukin-8 receptor B, IL-8R B, IL8RB, IL-8 receptor type 2, CD182
  • Anti-Human CXCR1 (extracellular) Antibody
    Anti-Human CXCR1 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to CXCR1 Chemokine Receptor
    • Product Code: ACR-011
    • Short Description: A Rabbit Polyclonal Antibody to CXCR1 Chemokine Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot
    • Target: C-X-C chemokine receptor type 1, Interleukin-8 receptor A, IL-8R A, IL8RA, IL-8 receptor type 1, CD181
  • Anti-Calcium Sensing Receptor (extracellular)-FITC Antibody
    Anti-Calcium Sensing Receptor (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to Calcium Sensing Receptor Conjugated to the Fluorescent Dye FITC
    • Product Code: ACR-004-F
    • Short Description: A Rabbit Polyclonal Antibody to Calcium Sensing Receptor Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: CaSR, Extracellular calcium-sensing receptor, Parathyroid cell calcium-sensing receptor 1, PCaR1, GPRC2A
  • Anti-Calcium Sensing Receptor (extracellular) Antibody
    KO Validate'
    Anti-Calcium Sensing Receptor (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Calcium Sensing Receptor
    • Product Code: ACR-004
    • Short Description: A Rabbit Polyclonal Antibody to Calcium Sensing Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: CaSR, Extracellular calcium-sensing receptor, Parathyroid cell calcium-sensing receptor 1, PCaR1, GPRC2A
  • Anti-Human Cannabinoid Receptor 2 (extracellular) Antibody
    Anti-Human Cannabinoid Receptor 2 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Cannabinoid Receptor 2
    • Product Code: ACR-003
    • Short Description: A Rabbit Polyclonal Antibody to Cannabinoid Receptor 2
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence
    • Target: CB2, CNR2
  • Anti-Cannabinoid Receptor 2 Antibody
    Anti-Cannabinoid Receptor 2 Antibody
    A Rabbit Polyclonal Antibody to Cannabinoid Receptor 2
    • Product Code: ACR-002
    • Short Description: A Rabbit Polyclonal Antibody to Cannabinoid Receptor 2
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: CB2, CNR2
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