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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

2041 Results
  • Anti-Ca<sub>V</sub>1.1 (CACNA1S) (extracellular) Antibody
    Anti-CaV1.1 (CACNA1S) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to CaV1.1 (CACNA1S) Channel
    • Product Code: ACC-314
    • Short Description: A Rabbit Polyclonal Antibody to CaV1.1 (CACNA1S) Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: Voltage-dependent L-type calcium channel subunit α1S, CACH1, CACN1, CACH1B, CACNL1A3
  • Anti-Ca<sub>V</sub>1.3 (CACNA1D) (extracellular)-FITC Antibody
    Anti-CaV1.3 (CACNA1D) (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to CaV1.3 (CACNA1D) (extracellular) conjugated to the fluorescent dye FITC
    • Product Code: ACC-311-F
    • Short Description: A Rabbit Polyclonal Antibody to CaV1.3 (CACNA1D) (extracellular) conjugated to the fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Voltage-dependent L-type calcium channel subunit α1D
  • Anti-Ca<sub>V</sub>1.3 (CACNA1D) (extracellular) Antibody
    Anti-CaV1.3 (CACNA1D) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to CaV1.3 (CACNA1D) Channel
    • Product Code: ACC-311
    • Short Description: A Rabbit Polyclonal Antibody to CaV1.3 (CACNA1D) Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Voltage-dependent L-type calcium channel subunit α1D
  • Anti-CatSper4 (extracellular) Antibody
    Anti-CatSper4 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to CatSper4 Channel
    • Product Code: ACC-304
    • Short Description: A Rabbit Polyclonal Antibody to CatSper4 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence
    • Target: Cation channel sperm-associated protein 4
  • Anti-CatSper1 (extracellular) Antibody
    Anti-CatSper1 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to CatSper1 Channel
    • Product Code: ACC-301
    • Short Description: A Rabbit Polyclonal Antibody to CatSper1 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot
    • Target: Cation channel sperm-associated protein 1
  • Guinea Pig Anti-Pannexin 1 Antibody
    Guinea Pig Anti-Pannexin 1 Antibody
    A Guinea Pig Polyclonal Antibody to Pannexin 1
    • Product Code: ACC-234-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to Pannexin 1
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: PANX1, PX1, MRS1, UNQ2529
  • Anti-Pannexin 1 Antibody
    KO Validate'
    Anti-Pannexin 1 Antibody
    A Rabbit Polyclonal Antibody to Pannexin 1
    • Product Code: ACC-234
    • Short Description: A Rabbit Polyclonal Antibody to Pannexin 1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: PANX1, PX1, MRS1, UNQ2529
  • Anti-Connexin-26 (GJB2) Antibody
    Anti-Connexin-26 (GJB2) Antibody
    A Rabbit Polyclonal Antibody to Connexin-26
    • Product Code: ACC-212
    • Short Description: A Rabbit Polyclonal Antibody to Connexin-26
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: CX26, Gap junction β2 protein
  • Anti-Connexin-32 Antibody
    Anti-Connexin-32 Antibody
    A Rabbit Polyclonal Antibody to Connexin-32
    • Product Code: ACC-211
    • Short Description: A Rabbit Polyclonal Antibody to Connexin-32
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot
    • Target: CX32, Gap junction β1 protein, GJB1, CMTX
  • Anti-Connexin-40 (GJA5) Antibody
    Anti-Connexin-40 (GJA5) Antibody
    A Rabbit Polyclonal Antibody to Connexin-40
    • Product Code: ACC-205
    • Short Description: A Rabbit Polyclonal Antibody to Connexin-40
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot
    • Target: CX40, Gap junction α5 protein
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