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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

2041 Results
  • Anti-Connexin-37 Antibody
    Anti-Connexin-37 Antibody
    A Rabbit Polyclonal Antibody to Connexin-37
    • Product Code: ACC-204
    • Short Description: A Rabbit Polyclonal Antibody to Connexin-37
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot
    • Target: CX37, Gap junction α4 protein, GJA4
  • Guinea pig Anti-Connexin-43 Antibody
    Guinea pig Anti-Connexin-43 Antibody
    A Guinea Pig Polyclonal Antibody to Connexin-43
    • Product Code: ACC-201-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to Connexin-43
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: CX43, Gap junction α1 protein, GJA1, GJAL, Gap junction 43 kDa heart protein
  • Anti-Connexin-43 Antibody
    Anti-Connexin-43 Antibody
    A Rabbit Polyclonal Antibody to Connexin-43
    • Product Code: ACC-201
    • Short Description: A Rabbit Polyclonal Antibody to Connexin-43
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: CX43, Gap junction α1 protein, GJA1, GJAL, Gap junction 43 kDa heart protein
  • Anti-Human TRPV2 (VRL1) (extracellular)-FITC Antibody
    Anti-Human TRPV2 (VRL1) (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to Human TRPV2 (extracellular) conjugated to the fluorescent dye FITC
    • Product Code: ACC-139-F
    • Short Description: A Rabbit Polyclonal Antibody to Human TRPV2 (extracellular) conjugated to the fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human
    • Applications: Live cell imaging, Flow cytometry
    • Target: Vanilloid receptor-like protein 1, VRL-1, Osm-9-like TRP channel 2, OTRPC2
  • Anti-Human TRPV2 (VRL1) (extracellular) Antibody
    Anti-Human TRPV2 (VRL1) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to TRPV2
    • Product Code: ACC-139
    • Short Description: A Rabbit Polyclonal Antibody to TRPV2
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot
    • Target: Vanilloid receptor-like protein 1, VRL-1, Osm-9-like TRP channel 2, OTRPC2, Strech-activated channel 2B, SAC2B
  • Anti-Ca<sub>V</sub>1.2 (CACNA1C) (extracellular) Antibody
    Anti-CaV1.2 (CACNA1C) (extracellular) Antibody
    A Rabbit Polyclonal antibody to CaV1.2 (extracellular)
    • Product Code: ACC-130
    • Short Description: A Rabbit Polyclonal antibody to CaV1.2 (extracellular)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: Voltage-dependent L-type calcium channel subunit alpha-1C, Calcium Voltage-Gated Channel Subunit Alpha1C
  • Anti-TRPM7 (extracellular)-FITC Antibody
    Anti-TRPM7 (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to TRPM7 (extracellular) conjugated to the fluorescent dye FITC
    • Product Code: ACC-129-F
    • Short Description: A Rabbit Polyclonal Antibody to TRPM7 (extracellular) conjugated to the fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Transient receptor potential cation channel subfamily M member 7, Long transient receptor potential channel 7, LTRPC7, Channel-kinase 1, CHAK1, Transient receptor potential-phospholipase C-interacting kinase, TRP-PLIK
  • Anti-TRPM7 (extracellular) Antibody
    Anti-TRPM7 (extracellular) Antibody
    A Rabbit Polyclonal antibody to TRPM7 (extracellular)
    • Product Code: ACC-129
    • Short Description: A Rabbit Polyclonal antibody to TRPM7 (extracellular)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: Transient receptor potential cation channel subfamily M member 7, Long transient receptor potential channel 7, LTRPC7, Channel-kinase 1, CHAK1, Transient receptor potential-phospholipase C-interacting kinase, TRP-PLIK
  • Anti-TRPM2 (extracellular)-FITC Antibody
    Anti-TRPM2 (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to TRPM2 Channel conjugated to the fluorescent dye FITC
    • Product Code: ACC-128-F
    • Short Description: A Rabbit Polyclonal Antibody to TRPM2 Channel conjugated to the fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Transient receptor potential cation channel subfamily M member 2, Transient receptor potential melastatin 2, LTrpC2, TrpC7
  • Anti-TRPM2 (extracellular) Antibody
    Anti-TRPM2 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to TRPM2 Channel
    • Product Code: ACC-128
    • Short Description: A Rabbit Polyclonal Antibody to TRPM2 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: Transient receptor potential cation channel subfamily M member 2, Transient receptor potential melastatin 2, LTrpC2, TrpC7
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