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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

2041 Results
  • Anti-CACNB2-ATTO Fluor-594 Antibody
    Anti-CACNB2-ATTO Fluor-594 Antibody
    A Rabbit Polyclonal Antibody to CACNB2 (CaVβ2) Conjugated to the Fluorescent Dye ATTO-594
    • Product Code: ACC-105-AR
    • Short Description: A Rabbit Polyclonal Antibody to CACNB2 (CaVβ2) Conjugated to the Fluorescent Dye ATTO-594
    • Host: Rabbit
    • Label: ATTO 594
    • Reactivity: Rat, Mouse
    • Applications: Immunofluorescence, Immunohistochemistry
    • Target: CaVβ2, Voltage-dependent L-type calcium channel subunit beta-2, CACNLB2, CAB2, CACB2, Lambert-Eaton myasthenic syndrome antigen B, MYSB
  • Anti-CACNB2 Antibody
    Anti-CACNB2 Antibody
    A Rabbit Polyclonal Antibody to CACNB2 (CaVβ2) Subunit
    • Product Code: ACC-105
    • Short Description: A Rabbit Polyclonal Antibody to CACNB2 (CaVβ2) Subunit
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: CaVβ2, Voltage-dependent L-type calcium channel subunit beta-2, CACNLB2, CAB2, CACB2, Lambert-Eaton myasthenic syndrome antigen B, MYSB
  • Anti-CACNA2D3 (Ca<sub>V</sub>α2δ3) (extracellular)-ATTO Fluor-594 Antibody
    Anti-CACNA2D3 (CaVα2δ3) (extracellular)-ATTO Fluor-594 Antibody
    A Rabbit Polyclonal Antibody to CACNA2D3 (CaVα2δ3) Subunit Conjugated to the Fluorescent Dye ATTO-594
    • Product Code: ACC-103-AR
    • Short Description: A Rabbit Polyclonal Antibody to CACNA2D3 (CaVα2δ3) Subunit Conjugated to the Fluorescent Dye ATTO-594
    • Host: Rabbit
    • Label: ATTO 594
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Voltage-dependent calcium channel subunit alpha-2/delta-3
  • Anti-CACNA2D2 (Ca<sub>V</sub>α2δ2) (extracellular) Antibody
    Anti-CACNA2D2 (CaVα2δ2) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to CACNA2D2 (CaVα2δ2) Subunit
    • Product Code: ACC-102
    • Short Description: A Rabbit Polyclonal Antibody to CACNA2D2 (CaVα2δ2) Subunit
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Voltage-dependent calcium channel subunit alpha-2/delta-2
  • Anti-TRPML2 (Mucolipin-2) Antibody
    KO Validate'
    Anti-TRPML2 (Mucolipin-2) Antibody
    A Rabbit Polyclonal Antibody to TRPML2 (Mucolipin-2) Channel
    • Product Code: ACC-082
    • Short Description: A Rabbit Polyclonal Antibody to TRPML2 (Mucolipin-2) Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Mcoln2
  • Anti-TRPML1 (Mucolipin 1) Antibody
    KO Validate'
    Anti-TRPML1 (Mucolipin 1) Antibody
    A Rabbit Polyclonal Antibody to TRPML1 (Mucolipin 1) Channel
    • Product Code: ACC-081
    • Short Description: A Rabbit Polyclonal Antibody to TRPML1 (Mucolipin 1) Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: Mcoln1, Mucolipidin
  • Anti-TRPC7 (extracellular) Antibody
    Anti-TRPC7 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to TRPC7 Channel
    • Product Code: ACC-066
    • Short Description: A Rabbit Polyclonal Antibody to TRPC7 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence
    • Target: Transient receptor protein 7, TRP7
  • Anti-Orai3 Antibody
    Anti-Orai3 Antibody
    A Rabbit Polyclonal Antibody to Orai3 Channel
    • Product Code: ACC-065
    • Short Description: A Rabbit Polyclonal Antibody to Orai3 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot
    • Target: TMEM142C
  • Anti-STIM2 Antibody
    KO Validate'
    Anti-STIM2 Antibody
    A Rabbit Polyclonal Antibody to STIM2
    • Product Code: ACC-064
    • Short Description: A Rabbit Polyclonal Antibody to STIM2
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Stromal interaction molecule 2
  • Anti-STIM1 (extracellular)-ATTO Fluor-550 Antibody
    Anti-STIM1 (extracellular)-ATTO Fluor-550 Antibody
    A Rabbit Polyclonal Antibody to STIM1 Conjugated to the Fluorescent Dye ATTO-550
    • Product Code: ACC-063-AO
    • Short Description: A Rabbit Polyclonal Antibody to STIM1 Conjugated to the Fluorescent Dye ATTO-550
    • Host: Rabbit
    • Label: ATTO 550
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Immunocytochemistry, Immunofluorescence, Flow cytometry, Immunohistochemistry
    • Target: Stromal interaction molecule 1, GOK
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