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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

2041 Results
  • Anti-TRPC3-ATTO Fluor-594 Antibody
    KO Validate'
    Anti-TRPC3-ATTO Fluor-594 Antibody
    A Rabbit Polyclonal Antibody to TRPC3 Channel Conjugated to the Fluorescent Dye ATTO-594
    • Product Code: ACC-016-AR
    • Short Description: A Rabbit Polyclonal Antibody to TRPC3 Channel Conjugated to the Fluorescent Dye ATTO-594
    • Host: Rabbit
    • Label: ATTO 594
    • Reactivity: Human, Rat, Mouse
    • Applications: Immunofluorescence, Immunohistochemistry
    • Target: Short transient receptor potential channel 3, Transient receptor protein 3, TRP3
  • Anti-TRPC3 Antibody
    KO Validate'
    Anti-TRPC3 Antibody
    A Rabbit Polyclonal Antibody to TRPC3 Channel
    • Product Code: ACC-016
    • Short Description: A Rabbit Polyclonal Antibody to TRPC3 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Western blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry
    • Target: Short transient receptor potential channel 3, Transient receptor protein 3, TRP3
  • Anti-CACNA2D1 (Ca<sub>V</sub>α2δ1) (extracellular) Antibody
    KO Validate'
    Anti-CACNA2D1 (CaVα2δ1) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to CACNA2D1 (CaVα2δ1 Subunit)
    • Product Code: ACC-015
    • Short Description: A Rabbit Polyclonal Antibody to CACNA2D1 (CaVα2δ1 Subunit)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Mouse
    • Applications: Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence
    • Target: Voltage-dependent Ca2+ channel subunit alpha-2/delta-1, L-type calcium channel alpha 2 polypeptide, Dihydropyridine-sensitive L-type calcium channel alpha-2/delta subunit
  • Anti-Ca<sub>V</sub>1.2a (CACNA1C) Antibody
    Anti-CaV1.2a (CACNA1C) Antibody
    A Rabbit Polyclonal Antibody to CaV1.2a (CACNA1C) Channel
    • Product Code: ACC-013
    • Short Description: A Rabbit Polyclonal Antibody to CaV1.2a (CACNA1C) Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry
    • Target: Cardiac type α1C, Voltage-dependent L-type calcium channel subunit alpha-1C.
  • Anti-TRPC1 Antibody
    KO Validate'
    Anti-TRPC1 Antibody
    A Rabbit Polyclonal Antibody to TRPC1 Channel
    • Product Code: ACC-010
    • Short Description: A Rabbit Polyclonal Antibody to TRPC1 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry
    • Target: Short transient receptor potential channel 1, Transient receptor protein 1, TRP1
  • Anti-Ca<sub>V</sub>3.3 (CACNA1I) Antibody
    Anti-CaV3.3 (CACNA1I) Antibody
    A Rabbit Polyclonal Antibody to CaV3.3 (CACNA1I) Channel
    • Product Code: ACC-009
    • Short Description: A Rabbit Polyclonal Antibody to CaV3.3 (CACNA1I) Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Voltage-dependent T-type calcium channel subunit α1I
  • Anti-Ca<sub>V</sub>2.3 (CACNA1E) Antibody
    KO Validate'
    Anti-CaV2.3 (CACNA1E) Antibody
    A Rabbit Polyclonal Antibody to CaV2.3 (CACNA1E) Channel
    • Product Code: ACC-006
    • Short Description: A Rabbit Polyclonal Antibody to CaV2.3 (CACNA1E) Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: CACH6, CACNL1A6, Voltage-dependent R-type calcium channel subunit α1E, BII, Brain calcium channel II, RBE2
  • Guinea pig Anti-Ca<sub>V</sub>1.3 (CACNA1D) Antibody
    Guinea pig Anti-CaV1.3 (CACNA1D) Antibody
    A Guinea Pig Polyclonal Antibody to CaV1.3 (CACNA1D) Channel
    • Product Code: ACC-005-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to CaV1.3 (CACNA1D) Channel
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Voltage-dependent L-type calcium channel subunit α1D
  • Anti-Ca<sub>V</sub>1.3 (CACNA1D) Antibody
    KO Validate'
    Anti-CaV1.3 (CACNA1D) Antibody
    A Rabbit Polyclonal Antibody to CaV1.3 (CACNA1D) Channel
    • Product Code: ACC-005
    • Short Description: A Rabbit Polyclonal Antibody to CaV1.3 (CACNA1D) Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry
    • Target: Voltage-dependent L-type calcium channel subunit α1D
  • Anti-Ca<sub>V</sub>pan α1 Antibody
    Anti-CaVpan α1 Antibody
    A Rabbit Pan Polyclonal Antibody to CaV1 and CaV2 Subunits
    • Product Code: ACC-004
    • Short Description: A Rabbit Pan Polyclonal Antibody to CaV1 and CaV2 Subunits
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunoprecipitation, Immunohistochemistry
    • Target: Voltage-dependent L-type calcium channel subunit alpha-1C, Cacna1c
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