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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

2041 Results
  • Guinea pig Anti-Ca<sub>V</sub>1.2 (CACNA1C) Antibody
    Guinea pig Anti-CaV1.2 (CACNA1C) Antibody
    A Guinea Pig Polyclonal Antibody to CaV1.2 (CACNA1C) Channel
    • Product Code: ACC-003-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to CaV1.2 (CACNA1C) Channel
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Voltage-dependent L-type calcium channel subunit α1C
  • Anti-Ca<sub>V</sub>1.2 (CACNA1C)-ATTO Fluor-488 Antibody
    KO Validate'
    Anti-CaV1.2 (CACNA1C)-ATTO Fluor-488 Antibody
    A Rabbit Polyclonal Antibody to CaV1.2 (CACNA1C) Channel Conjugated to the Fluorescent Dye ATTO-488
    • Product Code: ACC-003-AG
    • Short Description: A Rabbit Polyclonal Antibody to CaV1.2 (CACNA1C) Channel Conjugated to the Fluorescent Dye ATTO-488
    • Host: Rabbit
    • Label: ATTO 488
    • Reactivity: Human, Rat, Mouse
    • Applications: Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Voltage-dependent L-type calcium channel subunit α1C
  • Anti-Ca<sub>V</sub>1.2 (CACNA1C) Antibody
    KO Validate'
    Anti-CaV1.2 (CACNA1C) Antibody
    A Rabbit Polyclonal Antibody to CaV1.2 (CACNA1C) Channel
    • Product Code: ACC-003
    • Short Description: A Rabbit Polyclonal Antibody to CaV1.2 (CACNA1C) Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Voltage-dependent L-type calcium channel subunit α1C
  • Anti-CACNA1B (Ca<sub>V</sub>2.2) Antibody
    KO Validate'
    Anti-CACNA1B (CaV2.2) Antibody
    A Rabbit Polyclonal Antibody to CACNA1B (CaV2.2) Channel
    • Product Code: ACC-002
    • Short Description: A Rabbit Polyclonal Antibody to CACNA1B (CaV2.2) Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry
    • Target: Voltage-dependent N-type calcium channel subunit α1B, Brain calcium channel III, BIII
  • Anti-CACNA1A (Ca<sub>V</sub>2.1) Antibody
    KO Validate'
    Anti-CACNA1A (CaV2.1) Antibody
    A Rabbit Polyclonal Antibody to CACNA1A (CaV2.1) Channel
    • Product Code: ACC-001
    • Short Description: A Rabbit Polyclonal Antibody to CACNA1A (CaV2.1) Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Voltage-dependent P/Q-type calcium channel subunit α1A, Brain calcium channel I, BI
  • Anti-ACVRL1 (extracellular) Antibody
    Anti-ACVRL1 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to ACVRL1
    • Product Code: ABR-041
    • Short Description: A Rabbit Polyclonal Antibody to ACVRL1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot
    • Target: Activin A Receptor Like Type 1, ALK1, Serine/Threonine-Protein Kinase Receptor R3, SKR3, TGF-B Superfamily Receptor Type I, TSR-I
  • Anti-TGR5 (GPBAR1) Antibody
    Anti-TGR5 (GPBAR1) Antibody
    A Rabbit Polyclonal Antibody to G-Protein Coupled Bile Acid Receptor 1 (TGR5)
    • Product Code: ABR-031
    • Short Description: A Rabbit Polyclonal Antibody to G-Protein Coupled Bile Acid Receptor 1 (TGR5)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot
    • Target: G-protein coupled bile acid receptor 1, M-BAR, GPR131, BG37
  • Anti-BAI1 (extracellular)-FITC Antibody
    Anti-BAI1 (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to BAI1 Conjugated to the Fluorescent Dye FITC
    • Product Code: ABR-021-F
    • Short Description: A Rabbit Polyclonal Antibody to BAI1 Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Brain-specific angiogenesis inhibitor 1, Adhesion G protein-coupled receptor B1, ADGRB1, GDAIF
  • Anti-BAI1 (extracellular) Antibody
    Anti-BAI1 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to BAI1
    • Product Code: ABR-021
    • Short Description: A Rabbit Polyclonal Antibody to BAI1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Brain-specific angiogenesis inhibitor 1, Adhesion G protein-coupled receptor B1, ADGRB1, GDAIF
  • Anti-B1 Bradykinin Receptor (BDKRB1) (extracellular)-FITC Antibody
    Anti-B1 Bradykinin Receptor (BDKRB1) (extracellular)-FITC Antibody
    A Rabbit Polyclonal antibody to Human BDKRB1(extracellular) conjugated to FITC fluorophore.
    • Product Code: ABR-013-F
    • Short Description: A Rabbit Polyclonal antibody to Human BDKRB1(extracellular) conjugated to FITC fluorophore.
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: BK-1 receptor, BKRB1, B1R, BKR2
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