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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

2041 Results
  • Anti-C3aR1 (extracellular)-FITC Antibody
    Anti-C3aR1 (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to C3aR1 (extracellular) conjugated to the fluorescent dye FITC
    • Product Code: AAR-036-F
    • Short Description: A Rabbit Polyclonal Antibody to C3aR1 (extracellular) conjugated to the fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Complement C3a Receptor 1, C3AR, C3a Anaphylatoxin Chemotactic Receptor
  • Anti-C3aR1 (extracellular) Antibody
    Anti-C3aR1 (extracellular) Antibody
    A Rabbit Polyclonal antibody to C3aR1 (extracellular)
    • Product Code: AAR-036
    • Short Description: A Rabbit Polyclonal antibody to C3aR1 (extracellular)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: Complement C3a Receptor 1, C3AR, C3a Anaphylatoxin Chemotactic Receptor
  • Anti-Adenosine A<sub>3</sub> Receptor (extracellular)-FITC Antibody
    Anti-Adenosine A3 Receptor (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to Adenosine A3 Receptor (extracellular) conjugated to the fluorescent dye FITC
    • Product Code: AAR-035-F
    • Short Description: A Rabbit Polyclonal Antibody to Adenosine A3 Receptor (extracellular) conjugated to the fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: ADORA3, A3AR, Adenosine Receptor A3
  • Anti-Adenosine A<sub>3</sub> Receptor (extracellular) Antibody
    Anti-Adenosine A3 Receptor (extracellular) Antibody
    A Rabbit Polyclonal antibody to Adenosine A3 Receptor (extracellular)
    • Product Code: AAR-035
    • Short Description: A Rabbit Polyclonal antibody to Adenosine A3 Receptor (extracellular)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot
    • Target: ADORA3, A3AR, Adenosine Receptor A3
  • Anti-C5aR2 (extracellular) Antibody
    Anti-C5aR2 (extracellular) Antibody
    A Rabbit Polyclonal antibody to C5aR2 (extracellular).
    • Product Code: AAR-034
    • Short Description: A Rabbit Polyclonal antibody to C5aR2 (extracellular).
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Indirect flow cytometry, Western blot, Immunohistochemistry
    • Target: Complement Component 5a Receptor 2, C5L2, C5a Anaphylatoxin Chemotactic Receptor 2, G Protein-Coupled Receptor 77, GPR77
  • Anti-Human C5aR2 (extracellular) Antibody
    Anti-Human C5aR2 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to C5a Anaphylatoxin Receptor 2
    • Product Code: AAR-033
    • Short Description: A Rabbit Polyclonal Antibody to C5a Anaphylatoxin Receptor 2
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot
    • Target: C5a anaphylatoxin chemotactic receptor 2, C5a anaphylatoxin receptor 2, Complement component 5a receptor 2, G-protein coupled receptor 77, C5L2, GPR77
  • Anti-Human C5aR1 (extracellular) Antibody
    Anti-Human C5aR1 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to C5a Anaphylatoxin Receptor
    • Product Code: AAR-032
    • Short Description: A Rabbit Polyclonal Antibody to C5a Anaphylatoxin Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot
    • Target: C5a1 receptor, C5a anaphylatoxin chemotactic receptor 1, C5a anaphylatoxin receptor, C5R1, Complement component 5a receptor 1, CD88
  • Anti-Human C3aR (extracellular) Antibody
    Anti-Human C3aR (extracellular) Antibody
    A Rabbit Polyclonal Antibody to C3a Anaphylatoxin Receptor
    • Product Code: AAR-031
    • Short Description: A Rabbit Polyclonal Antibody to C3a Anaphylatoxin Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot
    • Target: C3a anaphylatoxin chemotactic receptor, C3a anaphylatoxin receptor, Complement component 3a receptor 1, C3a receptor, C3AR1, C3R1, AZ3B, HNFAG09
  • Anti-Apelin/APJ Receptor (extracellular) Antibody
    Anti-Apelin/APJ Receptor (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Apelin Receptor
    • Product Code: AAR-030
    • Short Description: A Rabbit Polyclonal Antibody to Apelin Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Western blot, Immunofluorescence, Immunohistochemistry
    • Target: APLNR, Angiotensin receptor-like 1, AGTRL1, G-protein coupled receptor APJ, GPCR34
  • Anti-Human Adenosine A3 Receptor (extracellular) Antibody
    Anti-Human Adenosine A3 Receptor (extracellular) Antibody
    A rabbit polyclonal antibody to Human Adenosine A3 Receptor (extracellular)
    • Product Code: AAR-024
    • Short Description: A rabbit polyclonal antibody to Human Adenosine A3 Receptor (extracellular)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot
    • Target: ADORA3
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