Epilepsy

Epilepsy

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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

790 Results
  • Anti-KCNH7 (erg3) Antibody
    Anti-KCNH7 (erg3) Antibody
    A Rabbit Polyclonal Antibody to KV11.3 (ERG-3) Channel
    • Product Code: APC-112
    • Short Description: A Rabbit Polyclonal Antibody to KV11.3 (ERG-3) Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: KV11.3, Potassium voltage-gated channel subfamily H member 7, Ether-a-go-go-related channel 3, Eag-related protein 3, ERG-3, hERG3
  • Anti-KCNJ15 (K<sub>ir</sub>4.2) Antibody
    Anti-KCNJ15 (Kir4.2) Antibody
    A Rabbit Polyclonal Antibody to KCNJ15 (Kir4.2) Channel
    • Product Code: APC-058
    • Short Description: A Rabbit Polyclonal Antibody to KCNJ15 (Kir4.2) Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: ATP-sensitive inward rectifier potassium channel 15, Potassium channel inwardly rectifying subfamily J member 15, Kir1.3, IRKK
  • Anti-KCNK1 (TWIK-1) (extracellular) Antibody
    Anti-KCNK1 (TWIK-1) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to KCNK1 Channel
    • Product Code: APC-110
    • Short Description: A Rabbit Polyclonal Antibody to KCNK1 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Potassium channel subfamily K member 1, Inward rectifying K+ channel protein TWIK-1, K2P1.1, HOHO1, KCNO1
  • Anti-KCNK10 (TREK-2) Antibody
    Anti-KCNK10 (TREK-2) Antibody
    A Rabbit Polyclonal Antibody to KCNK10 Channel
    • Product Code: APC-055
    • Short Description: A Rabbit Polyclonal Antibody to KCNK10 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Potassium channel subfamily K member 10, K2P10.1, TWIK-related K+ channel 2
  • Anti-KCNK12 (THIK-2) (extracellular) Antibody
    KO Validate'
    Anti-KCNK12 (THIK-2) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to KCNK12 Channel
    • Product Code: APC-169
    • Short Description: A Rabbit Polyclonal Antibody to KCNK12 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Potassium channel subfamily K member 12, Tandem pore domain halothane-inhibited K+ channel 2, K2P12.1
  • Anti-KCNK13 (THIK-1) (extracellular) Antibody
    Anti-KCNK13 (THIK-1) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to KCNK13 Channel
    • Product Code: APC-121
    • Short Description: A Rabbit Polyclonal Antibody to KCNK13 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: Potassium channel subfamily K member 13, Tandem pore domain halothane-inhibited K+ channel 1, K2P13.1
  • Anti-KCNK15 (TASK-5) (extracellular) Antibody
    Anti-KCNK15 (TASK-5) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to KCNK15 Channel
    • Product Code: APC-158
    • Short Description: A Rabbit Polyclonal Antibody to KCNK15 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot
    • Target: Potassium channel subfamily K member 15, Acid-sensitive K+ channel protein TASK-5, TWIK-related acid-sensitive K+ channel 5, K2P15.1
  • Anti-KCNK16 (TALK-1) (extracellular) Antibody
    KO Validate'
    Anti-KCNK16 (TALK-1) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to KCNK16 Channel
    • Product Code: APC-170
    • Short Description: A Rabbit Polyclonal Antibody to KCNK16 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: Potassium two pore domain channel subfamily K member 16, TWIK-related alkaline pH-activated K+ channel 1, K2P16.1
  • Anti-KCNK18 (TRESK) (extracellular) Antibody
    Anti-KCNK18 (TRESK) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to KCNK18 Channel
    • Product Code: APC-122
    • Short Description: A Rabbit Polyclonal Antibody to KCNK18 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Potassium channel subfamily K member 18, TWIK-related spinal cord K+ channel, K2P18.1
  • Anti-KCNK2 (TREK-1) Antibody
    KO Validate'
    Anti-KCNK2 (TREK-1) Antibody
    A Rabbit Polyclonal Antibody to KCNK2 Channel
    • Product Code: APC-047
    • Short Description: A Rabbit Polyclonal Antibody to KCNK2 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Potassium channel subfamily K member 2, Outward rectifying potassium channel protein TREK-1, Twik-related K+ channel 1, K2P2.1, TPKC1
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