Epilepsy

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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

790 Results
  • Anti-Neuroligin 2 (extracellular) Antibody
    Anti-Neuroligin 2 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Neuroligin 2
    • Product Code: ANR-036
    • Short Description: A Rabbit Polyclonal Antibody to Neuroligin 2
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: NLGN2, NL2
  • Anti-Neuroligin 3 (extracellular) Antibody
    Anti-Neuroligin 3 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Neuroligin 3
    • Product Code: ANR-037
    • Short Description: A Rabbit Polyclonal Antibody to Neuroligin 3
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: NLGN3, Gliotactin homolog, HNL3, NL3
  • Anti-Nicotinic Acetylcholine Receptor α2 (CHRNA2) (extracellular) Antibody
    Anti-Nicotinic Acetylcholine Receptor α2 (CHRNA2) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Nicotinic Acetylcholine Receptor α2
    • Product Code: ANC-002
    • Short Description: A Rabbit Polyclonal Antibody to Nicotinic Acetylcholine Receptor α2
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: nAChR α2, Cholinergic receptor nicotinic alpha 2, Neuronal acetylcholine receptor subunit alpha-2
  • Anti-Nicotinic Acetylcholine Receptor α4 (CHRNA4) (extracellular) Antibody
    KO Validate'
    Anti-Nicotinic Acetylcholine Receptor α4 (CHRNA4) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Nicotinic Acetylcholine Receptor α4
    • Product Code: ANC-004
    • Short Description: A Rabbit Polyclonal Antibody to Nicotinic Acetylcholine Receptor α4
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunofluorescence, Immunohistochemistry
    • Target: nAChR α4, Cholinergic receptor nicotinic alpha 4, NACRA4, NACHRA4, Neuronal acetylcholine receptor subunit alpha-4
  • Anti-Nicotinic Acetylcholine Receptor α7 (CHRNA7) (extracellular) Antibody
    KO Validate'
    Anti-Nicotinic Acetylcholine Receptor α7 (CHRNA7) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Nicotinic Acetylcholine Receptor α7
    • Product Code: ANC-007
    • Short Description: A Rabbit Polyclonal Antibody to Nicotinic Acetylcholine Receptor α7
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: nAChR α7, Cholinergic receptor nicotinic alpha 7, Neuronal acetylcholine receptor subunit alpha-7, ACRA7
  • Anti-Nicotinic Acetylcholine Receptor α7 (CHRNA7) (extracellular)-ATTO Fluor-633 Antibody
    Anti-Nicotinic Acetylcholine Receptor α7 (CHRNA7) (extracellular)-ATTO Fluor-633 Antibody
    A Rabbit Polyclonal Antibody to Nicotinic Acetylcholine Receptor α7 Conjugated to the Fluorescent Dye ATTO-633
    • Product Code: ANC-007-FR
    • Short Description: A Rabbit Polyclonal Antibody to Nicotinic Acetylcholine Receptor α7 Conjugated to the Fluorescent Dye ATTO-633
    • Host: Rabbit
    • Label: ATTO 633
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: nAChR α7, Cholinergic receptor nicotinic alpha 7, Neuronal acetylcholine receptor subunit alpha-7, ACRA7
  • Anti-Nicotinic Acetylcholine Receptor α7 (CHRNA7) (extracellular)-FITC Antibody
    Anti-Nicotinic Acetylcholine Receptor α7 (CHRNA7) (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to Nicotinic Acetylcholine Receptor α7 Conjugated to the Fluorescent Dye FITC
    • Product Code: ANC-007-F
    • Short Description: A Rabbit Polyclonal Antibody to Nicotinic Acetylcholine Receptor α7 Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: nAChR α7, Cholinergic receptor nicotinic alpha 7, Neuronal acetylcholine receptor subunit alpha-7, ACRA7
  • Anti-Nicotinic Acetylcholine Receptor β2 (CHRNB2) (extracellular) Antibody
    Anti-Nicotinic Acetylcholine Receptor β2 (CHRNB2) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Nicotinic Acetylcholine Receptor β2
    • Product Code: ANC-012
    • Short Description: A Rabbit Polyclonal Antibody to Nicotinic Acetylcholine Receptor β2
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: nAChR β2, Neuronal acetylcholine receptor subunit beta-2, Cholinergic receptor nicotinic beta 2, ACRB2, EFNL3
  • Anti-Nicotinic Acetylcholine Receptor β2 (CHRNB2) (extracellular)-ATTO Fluor-594 Antibody
    Anti-Nicotinic Acetylcholine Receptor β2 (CHRNB2) (extracellular)-ATTO Fluor-594 Antibody
    A Rabbit Polyclonal Antibody to Nicotinic Acetylcholine Receptor β2 Conjugated to the Fluorescent Dye ATTO-594
    • Product Code: ANC-012-AR
    • Short Description: A Rabbit Polyclonal Antibody to Nicotinic Acetylcholine Receptor β2 Conjugated to the Fluorescent Dye ATTO-594
    • Host: Rabbit
    • Label: ATTO 594
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: nAChR β2, Neuronal acetylcholine receptor subunit beta-2, Cholinergic receptor nicotinic beta 2, ACRB2, EFNL3
  • Anti-NKCC1 (SLC12A2) Antibody
    Anti-NKCC1 (SLC12A2) Antibody
    A Rabbit Polyclonal Antibody to NKCC1 Transporter
    • Product Code: ANT-071
    • Short Description: A Rabbit Polyclonal Antibody to NKCC1 Transporter
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Na+-K+-2Cl- Cotransporter 1, Basolateral Na-K-Cl symporter, Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 1, BSC2, Solute carrier family 12 member 2
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