Epilepsy

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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

790 Results
  • Anti-<em>slo</em>β4 (KCNMB4) Antibody
    Anti-sloβ4 (KCNMB4) Antibody
    A Rabbit Polyclonal Antibody to slo?4 (KCNMB4) Subunit
    • Product Code: APC-061
    • Short Description: A Rabbit Polyclonal Antibody to slo?4 (KCNMB4) Subunit
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Ca2+-activated K+ channel subunit β4, BKCa β subunit 4, BK beta4, Maxi K channel β4, Charybdotoxin receptor subunit beta-4
  • Anti-Stargazin Antibody
    Anti-Stargazin Antibody
    A Rabbit Polyclonal Antibody to Stargazin (CaV?2 Subunit)
    • Product Code: ACC-012
    • Short Description: A Rabbit Polyclonal Antibody to Stargazin (CaV?2 Subunit)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence
    • Target: CaVγ2, Voltage-dependent calcium channel gamma-2 subunit, CACNG2, TARP gamma-2, Transmembrane AMPAR regulatory protein gamma-2, Waggler
  • Anti-SynDIG1 Antibody
    Anti-SynDIG1 Antibody
    A Rabbit Polyclonal Antibody to SynDIG1
    • Product Code: AGC-049
    • Short Description: A Rabbit Polyclonal Antibody to SynDIG1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot
    • Target: Synapse differentiation-inducing gene protein 1, Transmembrane Protein 90B, TMEM90B, Dispanin subfamily C member 2, DSPC2
  • Anti-SynGAP Antibody
    Anti-SynGAP Antibody
    A Rabbit Polyclonal Antibody to SynGAP
    • Product Code: APZ-032
    • Short Description: A Rabbit Polyclonal Antibody to SynGAP
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: SynGAP1, Synaptic Ras GTPase-activating protein 1, Synaptic Ras-GAP 1, Neuronal RasGAP, p135 SynGAP
  • Anti-VGLUT3 Antibody
    Anti-VGLUT3 Antibody
    A Rabbit Polyclonal Antibody to Vesicular Glutamate Transporter 3
    • Product Code: AGC-037
    • Short Description: A Rabbit Polyclonal Antibody to Vesicular Glutamate Transporter 3
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Vesicular glutamate transporter 3, SLC17A8
  • APETx2
    Bioassay Tested
    APETx2
    A potent blocker of ASIC3 and moderate blocker of NaV1.8 channels
    • Product Code: STA-160
    • Short Description: A potent blocker of ASIC3 and moderate blocker of NaV1.8 channels
    • Applications: Electrophysiology
    • CAS No.: 713544-47-9
    • MW: 4561 Da
  • Arcaine sulfate
    Bioassay Tested
    Arcaine sulfate
    An Antagonist of NMDA Receptors
    • Product Code: A-285
    • Short Description: An Antagonist of NMDA Receptors
    • CAS No.: 14923-17-2
    • MW: 270.31
  • ASIC1 Blocking Peptide
    ASIC1 Blocking Peptide
    A Blocking Peptide for Anti-ASIC1 Antibody
    • Product Code: BLP-SC014
    • Short Description: A Blocking Peptide for Anti-ASIC1 Antibody
    • Applications: Western blot, Immunohistochemistry
    • Target: ASIC1
  • ASIC2a Blocking Peptide
    ASIC2a Blocking Peptide
    A Blocking Peptide for Anti-ASIC2a Antibody
    • Product Code: BLP-SC012
    • Short Description: A Blocking Peptide for Anti-ASIC2a Antibody
    • Applications: Western blot, Immunohistochemistry
    • Target: ASIC2
  • ASIC5 (extracellular) Blocking Peptide
    ASIC5 (extracellular) Blocking Peptide
    A Blocking Peptide for Anti-ASIC5 (extracellular) Antibody
    • Product Code: BLP-SC032
    • Short Description: A Blocking Peptide for Anti-ASIC5 (extracellular) Antibody
    • Applications: Western blot, Immunohistochemistry
    • Target: ASIC5
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