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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.
  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

790 Results
  • Anti-ASIC1 Antibody
    A Rabbit Polyclonal Antibody to ASIC1 Channel
    • Product Code: ASC-014
    • Short Description: A Rabbit Polyclonal Antibody to ASIC1 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry
    • Target: Acid-sensing ion channel 1, Amiloride-sensitive brain sodium channel, BNaC2, ACCN2
  • Anti-ASIC2a Antibody
    A Rabbit Polyclonal Antibody to ASIC2a Channel
    • Product Code: ASC-012
    • Short Description: A Rabbit Polyclonal Antibody to ASIC2a Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry
    • Target: ACCN1, Acid-sensing ion channel 2, Brain sodium channel 1, BNaC1, BNC1, Amiloride-sensitive cation channel neuronal 1, Mammalian degenerin homolog, MDEG
  • Anti-ASIC2a-ATTO Fluor-594 Antibody
    A Rabbit Polyclonal Antibody to ASIC2a Channel Conjugated to the Fluorescent Dye ATTO-594
    • Product Code: ASC-012-AR
    • Short Description: A Rabbit Polyclonal Antibody to ASIC2a Channel Conjugated to the Fluorescent Dye ATTO-594
    • Host: Rabbit
    • Label: ATTO 594
    • Reactivity: Human, Rat, Mouse
    • Applications: Immunofluorescence, Immunohistochemistry
    • Target: ACCN1, Acid-sensing ion channel 2, Brain sodium channel 1, BNaC1, BNC1, Amiloride-sensitive cation channel neuronal 1, Mammalian degenerin homolog, MDEG
  • Anti-ASIC5 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to ASIC5 Channel
    • Product Code: ASC-032
    • Short Description: A Rabbit Polyclonal Antibody to ASIC5 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Acid-sensing ion channel 5, Amiloride-sensitive cation channel 5, Brain-liver-intestine amiloride-sensitive Na+ channel, BLINaC, HINAC, ACCN5
  • Anti-CaBP1 Antibody
    A Rabbit Polyclonal Antibody to Calcium-Binding Protein 1
    • Product Code: ACS-002
    • Short Description: A Rabbit Polyclonal Antibody to Calcium-Binding Protein 1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot
    • Target: Calcium-binding protein 1, Caldendrin, Calbrain
  • Anti-CACNA1A (Ca<sub>V</sub>2.1) Antibody
    A Rabbit Polyclonal Antibody to CACNA1A (CaV2.1) Channel
    • Product Code: ACC-001
    • Short Description: A Rabbit Polyclonal Antibody to CACNA1A (CaV2.1) Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Voltage-dependent P/Q-type calcium channel subunit α1A, Brain calcium channel I, BI
  • Anti-CACNA1B (Ca<sub>V</sub>2.2) Antibody
    A Rabbit Polyclonal Antibody to CACNA1B (CaV2.2) Channel
    • Product Code: ACC-002
    • Short Description: A Rabbit Polyclonal Antibody to CACNA1B (CaV2.2) Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry
    • Target: Voltage-dependent N-type calcium channel subunit α1B, Brain calcium channel III, BIII
  • Anti-CACNA1G (Ca<sub>V</sub>3.1) Antibody
    A Rabbit Polyclonal Antibody to CACNA1G (CaV3.1) Channel
    • Product Code: ACC-021
    • Short Description: A Rabbit Polyclonal Antibody to CACNA1G (CaV3.1) Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Voltage-dependent T-type calcium channel subunit α1G
  • Anti-CACNA1G (Ca<sub>V</sub>3.1)-ATTO Fluor-594 Antibody
    A Rabbit Polyclonal Antibody to CACNA1G (CaV3.1) Conjugated to the Fluorescent Dye ATTO-594
    • Product Code: ACC-021-AR
    • Short Description: A Rabbit Polyclonal Antibody to CACNA1G (CaV3.1) Conjugated to the Fluorescent Dye ATTO-594
    • Host: Rabbit
    • Label: ATTO 594
    • Reactivity: Human, Rat, Mouse
    • Applications: Immunofluorescence, Immunohistochemistry
    • Target: Voltage-dependent T-type calcium channel subunit α1G
  • Anti-CACNA2D1 (Ca<sub>V</sub>α2δ1) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to CACNA2D1 (CaVα2δ1 Subunit)
    • Product Code: ACC-015
    • Short Description: A Rabbit Polyclonal Antibody to CACNA2D1 (CaVα2δ1 Subunit)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Mouse
    • Applications: Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence
    • Target: Voltage-dependent Ca2+ channel subunit alpha-2/delta-1, L-type calcium channel alpha 2 polypeptide, Dihydropyridine-sensitive L-type calcium channel alpha-2/delta subunit
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