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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.
  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

790 Results
  • Anti-CACNA2D2 (Ca<sub>V</sub>α2δ2) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to CACNA2D2 (CaVα2δ2) Subunit
    • Product Code: ACC-102
    • Short Description: A Rabbit Polyclonal Antibody to CACNA2D2 (CaVα2δ2) Subunit
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Voltage-dependent calcium channel subunit alpha-2/delta-2
  • Anti-CACNA2D3 (Ca<sub>V</sub>α2δ3) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to CACNA2D3 (CaVα2δ3) Subunit
    • Product Code: ACC-103
    • Short Description: A Rabbit Polyclonal Antibody to CACNA2D3 (CaVα2δ3) Subunit
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Voltage-dependent calcium channel subunit alpha-2/delta-3
  • Anti-CACNA2D3 (Ca<sub>V</sub>α2δ3) (extracellular)-ATTO Fluor-594 Antibody
    A Rabbit Polyclonal Antibody to CACNA2D3 (CaVα2δ3) Subunit Conjugated to the Fluorescent Dye ATTO-594
    • Product Code: ACC-103-AR
    • Short Description: A Rabbit Polyclonal Antibody to CACNA2D3 (CaVα2δ3) Subunit Conjugated to the Fluorescent Dye ATTO-594
    • Host: Rabbit
    • Label: ATTO 594
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Voltage-dependent calcium channel subunit alpha-2/delta-3
  • Anti-CACNA2D4 (Ca<sub>V</sub>α2δ4) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to CACNA2D4 (CaVα2δ4) Subunit
    • Product Code: ACC-104
    • Short Description: A Rabbit Polyclonal Antibody to CACNA2D4 (CaVα2δ4) Subunit
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Voltage-dependent calcium channel subunit alpha-2/delta-4, RCD4
  • Anti-CACNA2D4 (Ca<sub>V</sub>α2δ4) (extracellular)-ATTO Fluor-594 Antibody
    A Rabbit Polyclonal Antibody to CACNA2D4 (CaVα2δ4) Subunit Conjugated to the Fluorescent Dye ATTO-594
    • Product Code: ACC-104-AR
    • Short Description: A Rabbit Polyclonal Antibody to CACNA2D4 (CaVα2δ4) Subunit Conjugated to the Fluorescent Dye ATTO-594
    • Host: Rabbit
    • Label: ATTO 594
    • Reactivity: Rat, Mouse
    • Applications: Live cell imaging, Immunocytochemistry, Immunofluorescence
    • Target: Voltage-dependent calcium channel subunit alpha-2/delta-4, RCD4
  • Anti-CACNB1 Antibody
    A Rabbit Polyclonal Antibody to CACNB1 (CaVβ1) Subunit
    • Product Code: ACC-106
    • Short Description: A Rabbit Polyclonal Antibody to CACNB1 (CaVβ1) Subunit
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: CaVβ1, CAB1, CACNLB1, Voltage-dependent L-type calcium channel subunit beta-1
  • Anti-CACNB3 Antibody
    A Rabbit Polyclonal Antibody to CACNB3 (CaV?3) Subunit
    • Product Code: ACC-008
    • Short Description: A Rabbit Polyclonal Antibody to CACNB3 (CaV?3) Subunit
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: CaVβ3, CAB3, CACNLB3, Voltage-dependent L-type calcium channel subunit beta-3
  • Anti-CACNG1 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to CACNG1 (CaVγ1) Subunit
    • Product Code: ACC-111
    • Short Description: A Rabbit Polyclonal Antibody to CACNG1 (CaVγ1) Subunit
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: CaVγ1, CACNLG, Voltage-dependent calcium channel gamma-1 subunit, Dihydropyridine-sensitive L-type, skeletal muscle Ca2+ channel subunit gamma
  • Anti-CACNG3 Antibody
    A Rabbit Polyclonal Antibody to CACNG3 (CaVγ3) Subunit
    • Product Code: ACC-113
    • Short Description: A Rabbit Polyclonal Antibody to CACNG3 (CaVγ3) Subunit
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot
    • Target: CaVγ3, Voltage-dependent calcium channel gamma-3 subunit, TARP gamma-3, Transmembrane AMPAR regulatory protein gamma-3
  • Anti-CACNG4 Antibody
    A Rabbit Polyclonal Antibody to CACNG4 (CaVγ4) Subunit
    • Product Code: ACC-114
    • Short Description: A Rabbit Polyclonal Antibody to CACNG4 (CaVγ4) Subunit
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry
    • Target: CaVγ4, Voltage-dependent calcium channel gamma-4 subunit, TARP gamma-4, Transmembrane AMPAR regulatory protein gamma-4
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