Epilepsy

Epilepsy

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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

790 Results
  • Anti-HCN1 Antibody
    KO Validate'
    Anti-HCN1 Antibody
    A Rabbit Polyclonal Antibody to HCN1 Channel
    • Product Code: APC-056
    • Short Description: A Rabbit Polyclonal Antibody to HCN1 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Hyperpolarization-activated cyclic nucleotide-gated potassium channel 1, HAC2, Brain cyclic nucleotide-gated channel 1, BCNG1, Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1
  • Anti-HCN2 Antibody
    KO Validate'
    Anti-HCN2 Antibody
    A Rabbit Polyclonal Antibody to HCN2 Channel
    • Product Code: APC-030
    • Short Description: A Rabbit Polyclonal Antibody to HCN2 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry
    • Target: Hyperpolarization-activated cyclic nucleotide-gated potassium channel 2, BCNG2, HAC-1
  • Anti-HCN2-ATTO Fluor-594 Antibody
    KO Validate'
    Anti-HCN2-ATTO Fluor-594 Antibody
    A Rabbit Polyclonal Antibody to HCN2 Conjugated to the Fluorescent Dye ATTO-594
    • Product Code: APC-030-AR
    • Short Description: A Rabbit Polyclonal Antibody to HCN2 Conjugated to the Fluorescent Dye ATTO-594
    • Host: Rabbit
    • Label: ATTO 594
    • Reactivity: Human, Rat, Mouse
    • Applications: Immunofluorescence, Immunohistochemistry
    • Target: Hyperpolarization-activated cyclic nucleotide-gated potassium channel 2, BCNG2, HAC-1
  • Anti-HCN3 (extracellular) Antibody
    Anti-HCN3 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to HCN3 Channel
    • Product Code: APC-083
    • Short Description: A Rabbit Polyclonal Antibody to HCN3 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence
    • Target: K+/Na+ Hyperpolarization-activated cyclic nucleotide-gated channel 3, BCNG4, Hac3
  • Anti-HCN3 Antibody
    Anti-HCN3 Antibody
    A Rabbit Polyclonal Antibody to HCN3 Channel
    • Product Code: APC-057
    • Short Description: A Rabbit Polyclonal Antibody to HCN3 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: K+/Na+ Hyperpolarization-activated cyclic nucleotide-gated channel 3, BCNG4, Hac3
  • Anti-Homer3 Antibody
    Anti-Homer3 Antibody
    A Rabbit Polyclonal Antibody to Homer3
    • Product Code: APZ-028
    • Short Description: A Rabbit Polyclonal Antibody to Homer3
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Homer protein homolog 3, Homer scaffold protein 3, VESL3, Homer-3
  • Anti-Human GPR55 (extracellular) Antibody
    Anti-Human GPR55 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to GPR55
    • Product Code: ACR-061
    • Short Description: A Rabbit Polyclonal Antibody to GPR55
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human
    • Applications: Indirect flow cytometry, Western blot
    • Target: G-protein coupled receptor 55
  • Anti-Human Na<sub>V</sub>1.7 (SCN9A) (extracellular) Antibody
    Anti-Human NaV1.7 (SCN9A) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Human NaV1.7 (SCN9A)
    • Product Code: ASC-029
    • Short Description: A Rabbit Polyclonal Antibody to Human NaV1.7 (SCN9A)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry
    • Target: Voltage-gated sodium channel type IX subunit alpha, PN1
  • Anti-Human TRPM1 Antibody
    Anti-Human TRPM1 Antibody
    A Rabbit Polyclonal Antibody to Human TRPM1 Channel
    • Product Code: ACC-041
    • Short Description: A Rabbit Polyclonal Antibody to Human TRPM1 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human
    • Applications: Western blot
    • Target: Melastatin-1, MLSN1, LTrpC1, Transient receptor potential cation channel subfamily M member 1
  • Anti-KCC2 (SLC12A5) Antibody
    Anti-KCC2 (SLC12A5) Antibody
    A Rabbit Polyclonal Antibody to KCC2 Transporter
    • Product Code: AKT-005
    • Short Description: A Rabbit Polyclonal Antibody to KCC2 Transporter
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: K+-Cl- cotransporter 2, Electroneutral potassium-chloride cotransporter 2, Neuronal K-Cl cotransporter, Solute carrier family 12 member 5
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