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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.
  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

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790 Results
  • Anti-SCN4B (Na<sub>V</sub>β<sub>4</sub>) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to NaVβ4 Subunit
    • Product Code: ASC-044
    • Short Description: A Rabbit Polyclonal Antibody to NaVβ4 Subunit
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Voltage-gated sodium channel subunit beta-4, LQT10
  • Anti-SCN7A (Na<sub>X</sub>) Antibody
    A Rabbit Polyclonal antibody to SCN7A (NaX)
    • Product Code: ASC-050
    • Short Description: A Rabbit Polyclonal antibody to SCN7A (NaX)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: Sodium Channel Protein Type 7 Subunit Alpha, Putative voltage-gated sodium channel subunit alpha Nax, SCN6A, Nav2.1, Nav2.2
  • Anti-SEZ6 (extracellular) Antibody
    A Rabbit Polyclonal antibody to SEZ6
    • Product Code: ANR-206
    • Short Description: A Rabbit Polyclonal antibody to SEZ6
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: Seizure Protein 6, Seizure Related 6 Homolog
  • Anti-Shank1 Antibody
    A Rabbit Polyclonal Antibody to Shank1
    • Product Code: APZ-011
    • Short Description: A Rabbit Polyclonal Antibody to Shank1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: SH3 and multiple ankyrin repeat domains protein 1, SPANK-1, GKAP/SAPAP-interacting protein, Somatostatin receptor-interacting protein, SSTR-interacting protein, Synamon
  • Anti-Shank3 Antibody
    A Rabbit Polyclonal Antibody to Shank3
    • Product Code: APZ-013
    • Short Description: A Rabbit Polyclonal Antibody to Shank3
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: SH3 and multiple ankyrin repeat domains protein 3, SPANK-2, Proline-rich synapse-associated protein 2, ProSAP2
  • Anti-SLC1A7 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Excitatory Amino Acid Transporter 5 (SLC1A7)
    • Product Code: AGC-025
    • Short Description: A Rabbit Polyclonal Antibody to Excitatory Amino Acid Transporter 5 (SLC1A7)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot
    • Target: Excitatory amino acid transporter 5, EAAT5, Retinal glutamate transporter
  • Anti-SLC25A12 Antibody
    A Rabbit Polyclonal Antibody to SLC25A12
    • Product Code: ANT-112
    • Short Description: A Rabbit Polyclonal Antibody to SLC25A12
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: AGC1, ARALAR1, Solute carrier family 25 member 12, Mitochondrial aspartate glutamate carrier 1, Calcium-binding mitochondrial carrier protein Aralar1, Araceli hiperlarga
  • Anti-SLC25A18 (GC2) Antibody
    A Rabbit polyclonal antibody to SLC25A18 (GC2)
    • Product Code: ANT-118
    • Short Description: A Rabbit polyclonal antibody to SLC25A18 (GC2)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: Mitochondrial glutamate carrier 2, Glutamate/H(+) symporter 2, Solute carrier family 25 member 18, GC-2
  • Anti-SLC6A8 Antibody
    A Rabbit Polyclonal Antibody to Creatine Transporter
    • Product Code: AGT-008
    • Short Description: A Rabbit Polyclonal Antibody to Creatine Transporter
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Creatine transporter, Sodium- and chloride-dependent creatine transporter 1, CT1, CRT, CHOT1
  • Anti-<em>slo</em>β3 (KCNMB3) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to slo?3 (KCNMB3) Subunit
    • Product Code: APC-068
    • Short Description: A Rabbit Polyclonal Antibody to slo?3 (KCNMB3) Subunit
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: Ca2+-activated K+ channel subunit β3, BKCa β3, BK beta 3, Maxi K channel β3, Charybdotoxin receptor subunit beta-3
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